Similar articles

Abstract: Biological thiols (biothiols), an important kind of functional biomolecules, such as cysteine (Cys) and glutathione (GSH), play vital roles in maintaining the stability of the intracellular environment. In past decades, studies have demonstrated that metabolic disorder of biothiols is related to many serious disease processes and will lead to extreme damage in human and numerous animals. We carried out a series of experiments to detect biothiols in biosamples, including bovine plasma and cell lysates of seven different cell lines based on a simple colorimetric method. In a typical test, the color of the test solution could gradually change from blue to colorless after the addition of biothiols. Based on the color change displayed, experimental results reveal that the percentage of biothiols in the embryonic fibroblast cell line is significantly higher than those in the other six cell lines, which provides the basis for the following biothiols-related study.

生物巯化物的可视化检测

[1]Duan, L., Xu, Y., Qian, X., et al., 2008. Highly selective fluorescent chemosensor with red shift for cysteine in buffer solution and its bioimage: symmetrical naphthalimide aldehyde. Tetrahedron Lett., 49(47):6624-6627.

[2]Ghasemi, F., Hormozi-Nezhad, M.R., Mahmoudi, M., 2015. A colorimetric sensor array for detection and discrimination of biothiols based on aggregation of gold nanoparticles. Anal. Chim. Acta, 882:58-67.

[3]Heafield, M.T., Fearn, S., Steventon, G.B., et al., 1990. Plasma cysteine and sulphate levels in patients with motor neurone, Parkinson’s and Alzheimer’s disease. Neurosci. Lett., 110(1-2):216-220.

[4]Hong, V., Kislukhin, A.A., Finn, M., 2009. Thiol-selective fluorogenic probes for labeling and release. J. Am. Chem. Soc., 131(29):9986-9994.

[5]Lee, K.S., Kim, T.K., Lee, J.H., et al., 2008. Fluorescence turn-on probe for homocysteine and cysteine in water. Chem. Commun., 46:6173-6175.

[6]Lin, W., Long, L., Yuan, L., et al., 2008. A ratiometric fluorescent probe for cysteine and homocysteine displaying a large emission shift. Org. Lett., 10(24):5577-5580.

[7]Liu, J., Sun, Y.Q., Huo, Y., et al., 2014. Simultaneous fluorescence sensing of Cys and GSH from different emission channels. J. Am. Chem. Soc., 136(2):574-577.

[8]Ni, P., Sun, Y., Dai, H., et al., 2015. Highly sensitive and selective colorimetric detection of glutathione based on Ag [I] ion–3,3',5,5'-tetramethylbenzidine (TMB). Biosens. Bioelectron., 63:47-52.

[9]Ran, X., Sun, H., Pu, F., et al., 2013. Ag nanoparticle-decorated graphene quantum dots for label-free, rapid and sensitive detection of Ag⁺ and biothiols. Chem. Commun., 49(11):1079-1081.

[10]Reddie, K.G., Carroll, K.S., 2008. Expanding the functional diversity of proteins through cysteine oxidation. Curr. Opin. Chem. Biol., 12(6):746-754.

[11]Rusin, O., St. Luce, N.N., Agbaria, R.A., et al., 2004. Visual detection of cysteine and homocysteine. J. Am. Chem. Soc., 126(2):438-439.

[12]Shiu, H.Y., Chong, H.C., Leung, Y.C., et al., 2010. A highly selective FRET-based fluorescent probe for detection of cysteine and homocysteine. Chemistry, 16(11):3308-3313.

[13]Townsend, D.M., Tew, K.D., Tapiero, H., 2003. The importance of glutathione in human disease. Biomed. Pharmacother., 57(3):145-155.

[14]Wang, W., Rusin, O., Xu, X., et al., 2005. Detection of homocysteine and cysteine. J. Am. Chem. Soc., 127(45):15949-15958.

[15]Weerapana, E., Wang, C., Simon, G.M., et al., 2010. Quantitative reactivity profiling predicts functional cysteines in proteomes. Nature, 468(7325):790-795.

[16]Yang, J., Wang, H., Zhang, H., 2008. One-pot synthesis of silver nanoplates and charge-transfer complex nanofibers. J. Phys. Chem. C, 112(34):13065-13069.

[17]Yang, X., Guo, Y., Strongin, R.M., 2011. Conjugate addition/ cyclization sequence enables selective and simultaneous fluorescence detection of cysteine and homocysteine. Angew. Chem. Int. Ed., 50(45):10690-10693.

[18]Yang, X., Guo, Y., Strongin, R.M., 2012. A seminaphthofluorescein-based fluorescent chemodosimeter for the highly selective detection of cysteine. Org. Biomol. Chem., 10(14):2739-2741.

[19]Yi, L., Li, H., Sun, L., et al., 2009. A highly sensitive fluorescence probe for fast thiol-quantification assay of glutathione reductase. Angew. Chem. Int. Ed., 48(22):4034-4037.

[20]Zhang, L., Lu, B., Lu, C., et al., 2014. Determination of cysteine, homocysteine, cystine, and homocystine in biological fluids by HPLC using fluorosurfactant-capped gold nanoparticles as postcolumn colorimetric reagents. J. Sep. Sci., 37(1-2):30-36.

[21]Zhu, B., Guo, B., Zhao, Y., et al., 2014. A highly sensitive ratiometric fluorescent probe with a large emission shift for imaging endogenous cysteine in living cells. Biosens. Bioelectron., 55:72-75.