JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.3 P.199-210

In vitro antioxidant activity of phenolic-enriched extracts from Zhangping Narcissus tea cake and their inhibition on growth and metastatic capacity of 4T1 murine breast cancer cells

Author(s): Le Ying, De-dong Kong, Yuan-yuan Gao, Feng Yan, Yue-fei Wang, Ping Xu
Affiliation(s): Department of Tea Science, Zhejiang University, Hangzhou 310058, China; more
Corresponding email(s): zdxp@zju.edu.cn
Key Words: Oolong tea, Antioxidant activity, Metastasis, Cell cycle

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Abstract: Phenolics, as the main bioactive compounds in tea, have been suggested to have potential in the prevention of various human diseases. However, little is known about phenolics and their bioactivity in Zhangping Narcissue tea cake which is considered the most special kind of oolong tea. To unveil its bioactivity, three phenolic-enriched extracts were obtained from Zhangping Narcissue tea cake using ethyl acetate, n-butanol, and water. Their main chemical compositions and in vitro bioactivity were analyzed by high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). The ethyl acetate fraction (ZEF) consisted of higher content of phenolics, flavonoids, procyanidins, and catechin monomers (including epigallocatechin gallate (EGCG), epicatechin gallate (ECG), and gallocatechin gallate (GCG)) than n-butanol fraction (ZBF) and water fraction (ZWF). ZEF exhibited the strongest antioxidant capacity in vitro due to its abundant bioactive compounds. This was validated by Pearson correlation and hierarchical clustering analyses. ZEF also showed a remarkable inhibition on the growth, migration, and invasion of 4T1 murine breast cancer cells.

漳平水仙饼茶中酚类提取物的体外抗氧化活性及对小鼠乳腺癌4T1细胞增殖和转移的抑制作用

目的:探讨不同提取方法所得的漳平水仙饼茶中提取物的主要化学成分、体外抗氧化作用和对小鼠乳腺癌4T1细胞增殖和转移的抑制作用.
创新点:首次对漳平水仙饼茶中不同提取物的主要化学成分、体外抗氧化作用和对小鼠乳腺癌4T1细胞增殖和转移的抑制作用进行了研究.
方法:以漳平水仙饼茶为原料,利用乙酸乙酯、正丁醇和水分别进行萃取,得到三种提取物.对提取物的茶多酚、黄酮、原花青素、总糖、蛋白质、氨基酸、儿茶素单体、茶黄素、咖啡碱等主要化学成分进行检测.利用DPPH、ABTS和FRAP三种不同方法进一步检测了三种提取物的体外抗氧化性,并评价了提取物对小鼠乳腺癌4T1细胞的增殖、细胞周期、迁移、侵袭等的影响作用.同时,采用皮尔森系数分析和聚类分析两种分析方法探讨了提取物中的化学成分与其抗氧化活性和对4T1细胞作用的相关性.
结论:本实验结果显示,漳平水仙饼茶乙酸乙酯层提取物具有最高的茶多酚、黄酮、原花青素和儿茶素单体的含量(表1~3,图1),并且在三种不同的抗氧化体系中均显示出最强的抗氧化性能 (表4,图2),同时对小鼠乳腺癌4T1细胞的增殖、细胞周期、迁移、侵袭有非常明显的抑制作用(图3~6).乙酸乙酯层提取物之所以具有最强的生物活性主要与其含有较多的茶多酚等活性成分有关(表5,图3).
关键词：乌龙茶;抗氧化作用;转移;细胞周期

Reference

[1]Benzie IFF, Strain JJ, 1999. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol, 299:15-27.

[2]Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 72(1-2):248-254.

[3]Cai Y, Luo Q, Sun M, et al., 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci, 74(17):2157-2184.

[4]Chavan U, Shahidi F, Naczk M, 2001. Extraction of condensed tannins from beach pea (Lathyrus maritimus L.) as affected by different solvents. Food Chem, 75(4):509-512.

[5]Chen H, Qu Z, Fu L, et al., 2009. Physicochemical properties and antioxidant capacity of 3 polysaccharides from green tea, oolong tea, and black tea. J Food Sci, 74(6):C469-C474.

[6]Chen L, Ye HL, Zhang G, et al., 2014. Autophagy inhibition contributes to the synergistic interaction between EGCG and doxorubicin to kill the hepatoma Hep3B cells. PLoS ONE, 9(1):e85771.

[7]Chen Y, Lu B, Yang Q, et al., 2009. Combined integrin phosphoproteomic analyses and small interfering RNA-based functional screening identify key regulators for cancer cell adhesion and migration. Cancer Res, 69(8):3713-3720.

[8]Dou J, Lee VSY, Jason TC, et al., 2007. Identification and comparison of phenolic compounds in the preparation of oolong tea manufactured by semifermentation and drying processes. J Agric Food Chem, 55(18):7462-7468.

[9]Du GJ, Wang CZ, Qi LW, et al., 2013. The synergistic apoptotic interaction of panaxadiol and epigallocatechin gallate in human colorectal cancer cells. Phytother Res, 27(2):272-277.

[10]Finkel T, Holbrook NJ, 2000. Oxidants, oxidative stress and the biology of ageing. Nature, 408(6809):239-247.

[11]Fraser K, Harrison SJ, Lane GA, et al., 2012. HPLC-MS/MS profiling of proanthocyanidins in teas: a comparative study. J Food Compost Anal, 26(1-2):43-51.

[12]Garcia-Parrilla MC, Heredia FJ, Troncoso AM, et al., 1997. Spectrophotometric determination of total procyanidins in wine vinegars. Talanta, 44(1):119-123.

[13]Huang W, Deng Q, Xie B, et al., 2010. Purification and characterization of an antioxidant protein from ginkgo biloba seeds. Food Res Int, 43(1):86-94.

[14]Kaviarasan S, Naik GH, Gangabhagirathi R, et al., 2007. In vitro studies on antiradical and antioxidant activities of fenugreek (Trigonella foenum graecum) seeds. Food Chem, 103(1):31-37.

[15]Kim IS, Yang MR, Lee OH, et al., 2011. Antioxidant activities of hot water extracts from various spices. Int J Mol Sci, 12(12):4120-4131.

[16]Kuźma P, Drużyńska B, Obiedzinski M, 2014. Optimization of extraction conditions of some polyphenolic compounds from parsley leaves (Petroselinum crispum). Acta Sci Pol Technol Aliment, 13(2):145-154.

[17]Lai L, Fu Q, Liu Y, et al., 2012. Piperine suppresses tumor growth and metastasis in vitro and in vivo in a 4T1 murine breast cancer model. Acta Pharm Sin, 33(4):523-530.

[18]Lambert JD, Lee MJ, Lu H, et al., 2003. Epigallocatechin-3-gallate is absorbed but extensively glucuronidated following oral administration to mice. J Nutr, 133(12):4172-4177.

[19]Liang H, Liang Y, Dong J, et al., 2007. Decaffeination of fresh green tea leaf (Camellia sinensis) by hot water treatment. Food Chem, 101(4):1451-1456.

[20]Liu Z, Luo Z, Jia C, et al., 2016. Synergistic effects of Potentilla fruticosa L. leaves combined with green tea polyphenols in a variety of oxidation systems. J Food Sci, 81(5):C1091-C1101.

[21]Matsumoto N, Kohri T, Okushio K, et al., 1996. Inhibitory effects of tea catechins, black tea extract and oolong tea extract on hepatocarcinogenesis in rat. Jpn J Cancer Res, 87(10):1034-1038.

[22]Meda A, Lamien CE, Romito M, et al., 2005. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem, 91(3):571-577.

[23]Milenkovic D, Jude B, Morand C, 2013. MiRNA as molecular target of polyphenols underlying their biological effects. Free Radical Biol Med, 64:40-51.

[24]Mohsen SM, Ammar ASM, 2009. Total phenolic contents and antioxidant activity of corn tassel extracts. Food Chem, 112(3):595-598.

[25]Monbaliu S, Wu A, Zhang D, et al., 2010. Multimycotoxin UPLC-MS/MS for tea, herbal infusions and the derived drinkable products. J Agric Food Chem, 58(24):12664-12671.

[26]Morris DL, 1948. Quantitative determination of carbohydrates with Dreywood’s anthrone reagent. Science, 107(2775):254-255.

[27]Shi J, Gong J, Liu J, et al., 2009. Antioxidant capacity of extract from edible flowers of prunus mume in China and its active components. LWT Food Sci Technol, 42(2):477-482.

[28]Singh R, Singh S, Kumar S, et al., 2007. Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn. Food Chem Toxicol, 45(7):1216-1223.

[29]Sun SW, Lin YC, Weng YM, et al., 2006. Efficiency improvements on ninhydrin method for amino acid quantification. J Food Compost Anal, 19(2-3):112-117.

[30]Tanaka T, Matsuo Y, Kouno I, 2009. Chemistry of secondary polyphenols produced during processing of tea and selected foods. Int J Mol Sci, 11(1):14-40.

[31]Wang Y, Bian X, Park J, et al., 2011. Physicochemical properties, in vitro antioxidant activities and inhibitory potential against α-glucosidase of polysaccharides from Ampelopsis grossedentata leaves and stems. Molecules, 16(12):7762-7772.

[32]Yang CS, Lambert JD, Sang S, 2009. Antioxidative and anti-carcinogenic activities of tea polyphenols. Arch Toxicol, 83(1):11-21.

[33]Yang Y, Qiao L, Zhang X, et al., 2015. Effect of methylated tea catechins from Chinese oolong tea on the proliferation and differentiation of 3T3-L1 preadipocyte. Fitoterapia, 104:45-49.

[34]Yuan X, Yu L, Li J, et al., 2013. ATF3 suppresses metastasis of bladder cancer by regulating gelsolin-mediated remodeling of the actin cytoskeleton. Cancer Res, 73(12):3625-3637.

[35]List of electronic supplementary materials

[36]Fig. S1 Flow chart of phenolic-enriched extracts from Zhangping Narcissus tea cake

[37]Fig. S2 UPLC-MS chromatograms of ZBF

[38]Fig. S3 UPLC-MS chromatograms of ZEF

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漳平水仙饼茶中酚类提取物的体外抗氧化活性及对小鼠乳腺癌4T1细胞增殖和转移的抑制作用

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