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Hypoxia promotes ovarian cancer progression via Lnc-DARS-AS1/miR-378a-5p/SLC2A12 axis
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Wenjie ZENG1,2,3,4, Hubin XU5, Huafeng SHOU2,3,4, Haimin JIANG5, Mengyu ZHANG5, Xiaoyan CHEN2, Weiguo LU1,6,7. Hypoxia promotes ovarian cancer progression via Lnc-DARS-AS1/miR-378a-5p/SLC2A12 axis[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .
@article{title="Hypoxia promotes ovarian cancer progression via Lnc-DARS-AS1/miR-378a-5p/SLC2A12 axis",
author="Wenjie ZENG1,2,3,4, Hubin XU5, Huafeng SHOU2,3,4, Haimin JIANG5, Mengyu ZHANG5, Xiaoyan CHEN2, Weiguo LU1,6,7",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2500083"
}
%0 Journal Article
%T Hypoxia promotes ovarian cancer progression via Lnc-DARS-AS1/miR-378a-5p/SLC2A12 axis
%A Wenjie ZENG1
%A 2
%A 3
%A 4
%A Hubin XU5
%A Huafeng SHOU2
%A 3
%A 4
%A Haimin JIANG5
%A Mengyu ZHANG5
%A Xiaoyan CHEN2
%A Weiguo LU1
%A 6
%A 7
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
%P
%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2500083
TY - JOUR
T1 - Hypoxia promotes ovarian cancer progression via Lnc-DARS-AS1/miR-378a-5p/SLC2A12 axis
A1 - Wenjie ZENG1
A1 - 2
A1 - 3
A1 - 4
A1 - Hubin XU5
A1 - Huafeng SHOU2
A1 - 3
A1 - 4
A1 - Haimin JIANG5
A1 - Mengyu ZHANG5
A1 - Xiaoyan CHEN2
A1 - Weiguo LU1
A1 - 6
A1 - 7
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2500083
Abstract: To examine hypoxia-induced alterations in ovarian cancer and elucidate the underlying molecular mechanisms mediated by long non-coding RNAs (lncRNAs). Transwell and MTT assays were performed to assess the migration, invasion and proliferative abilities of ovarian cancer cells. Western blot analysis was conducted to evaluate the expression of factors associated with epithelial-mesenchymal transition (EMT). RNA sequencing was carried out to identify potential targets, and dual-luciferase and biotin-labeled miRNA pull-down assays were performed to identify interactions between these targets. Gain- and loss-of-function experiments were conducted to validate their effects. hypoxia promoted the migration, invasion, proliferation, and EMT of HO8910 and A2780 cells. Under hypoxic conditions, the expression of Lnc-DARS-AS1 was significantly increased, and this upregulation exerted a proliferative effect on ovarian cancer cells. Furthermore, Lnc-DARS-AS1 promoted solute carrier family 2 member 12 (SLC2A12) expression, accelerating ovarian cancer progression. Luciferase, biotin-labeled miRNA pull-down, and cellular function assays confirmed that Lnc-DARS-AS1 binds to miR-378a-5p to enhance SLC2A12 levels. The expression levels of SLC2A12 were determined in samples from patients with ovarian cancer, and they showed a positive correlation with cancer progression. This research established that the Lnc-DARS-AS1/miR-378a-5p/SLC2A12 axis is involved in ovarian cancer progression under hypoxic conditions, providing new perspectives on the treatment and management of ovarian cancer.
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