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Journal of Zhejiang University SCIENCE A 2002 Vol.3 No.5 P.622-626

http://doi.org/10.1631/jzus.2002.0622


Use of flow cytometry to investigate the cytokine response pattern in infants with respiratory syncytial virus infection and bronchiolitis


Author(s):  CHEN Zhi-min, DU Li-zhong, MAO Jian-hua, TANG Yong-min

Affiliation(s):  The Children's Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   chenzm@mail.hz.zj.cn

Key Words:  Respiratory syncytial virus (RSV), Bronchiolitis, IL-4/IFN-γ


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CHEN Zhi-min, DU Li-zhong, MAO Jian-hua, TANG Yong-min. Use of flow cytometry to investigate the cytokine response pattern in infants with respiratory syncytial virus infection and bronchiolitis[J]. Journal of Zhejiang University Science A, 2002, 3(5): 622-626.

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author="CHEN Zhi-min, DU Li-zhong, MAO Jian-hua, TANG Yong-min",
journal="Journal of Zhejiang University Science A",
volume="3",
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pages="622-626",
year="2002",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2002.0622"
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%T Use of flow cytometry to investigate the cytokine response pattern in infants with respiratory syncytial virus infection and bronchiolitis
%A CHEN Zhi-min
%A DU Li-zhong
%A MAO Jian-hua
%A TANG Yong-min
%J Journal of Zhejiang University SCIENCE A
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%P 622-626
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2002.0622

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T1 - Use of flow cytometry to investigate the cytokine response pattern in infants with respiratory syncytial virus infection and bronchiolitis
A1 - CHEN Zhi-min
A1 - DU Li-zhong
A1 - MAO Jian-hua
A1 - TANG Yong-min
J0 - Journal of Zhejiang University Science A
VL - 3
IS - 5
SP - 622
EP - 626
%@ 1869-1951
Y1 - 2002
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2002.0622


Abstract: 
Objective: To investigate the cytokine response pattern (IL-4/IFN-γ;) in infants with RSV infections and bronchiolitis during the acute phase. Methods: Four-color flow cytometry was used to measure intracellular IL-4 and IFN-γ expressions in peripheral blood CD3+ and CD8+ lymphocytes from RSV-infected and bronchiolitis infants. Serum IL-4 and IFN-γ levels were also determined. Results: RSV-infected and bronchiolitis infants showed no statistical differences from not-RSV-infected or pneumonia infants and control in the frequency of IL-4 and IFN-γ expressions in CD3+CD8- lymphocytes, showed no obvious Th1/Th2 imbalance, while IFN-γ was expressed much more frequently in CD3+CD8+ lymphocytes. Systematically, RSV-infected and bronchiolitis infants showed much lower levels of serum IL-4 and IL-4/IFN-γ; ratios and much higher serum IFN-γ levels than control. However, there were no statistical differences in the above three indices between RSV-infected and not-RSV infected infants or between bronchiolitis and pneumonia infants, except that bronchiolitis infants had a higher level of serum IFN-γ than pneumonia infants statistically. Conclusions: There is no type-2 cytokine response predominance in the acute phase of RSV infection and bronchiolitis. IL-4 production is suppressed and IFN-γ production upregulated, the latter being most prominent in bronchiolitis infants.

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Reference

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