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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.4 P.467-471

http://doi.org/10.1631/jzus.2004.0467


Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant


Author(s):  CHEN Jian-zhong, ZHU Hai-hong, LIU Ke-zhou, CHEN Zhi

Affiliation(s):  Institute of Immunology, School of Medicine, Zhejiang University, Hangzhou 310031, China; more

Corresponding email(s):   chenjzh@mail.hz.zj.cn

Key Words:  Interleukin-18, Hepatitis B virus, DNA vaccines, Immune response


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CHEN Jian-zhong, ZHU Hai-hong, LIU Ke-zhou, CHEN Zhi. Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant[J]. Journal of Zhejiang University Science A, 2004, 5(4): 467-471.

@article{title="Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant",
author="CHEN Jian-zhong, ZHU Hai-hong, LIU Ke-zhou, CHEN Zhi",
journal="Journal of Zhejiang University Science A",
volume="5",
number="4",
pages="467-471",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0467"
}

%0 Journal Article
%T Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant
%A CHEN Jian-zhong
%A ZHU Hai-hong
%A LIU Ke-zhou
%A CHEN Zhi
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 4
%P 467-471
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0467

TY - JOUR
T1 - Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant
A1 - CHEN Jian-zhong
A1 - ZHU Hai-hong
A1 - LIU Ke-zhou
A1 - CHEN Zhi
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 4
SP - 467
EP - 471
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.0467


Abstract: 
Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M,the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitrowas significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.

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