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Journal of Zhejiang University SCIENCE A 2003 Vol.4 No.2 P.142-151

http://doi.org/10.1631/jzus.2003.0142


Influence of medium components on elastase production using crude sources by Bacillus sp. EL31410


Author(s):  HE Guo-qing, CHEN Qi-he, ZHANG Li, LIU Xiao-jie

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   gqhe@zju.edu.cn

Key Words:  Elastase, Bacillus sp. EL31410, Crude sources, Medium optimization, Fractional factorial design (FFD), Central composite design (CCD), Response surface methodology (RSM), Batch cultivation


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HE Guo-qing, CHEN Qi-he, ZHANG Li, LIU Xiao-jie. Influence of medium components on elastase production using crude sources by Bacillus sp. EL31410[J]. Journal of Zhejiang University Science A, 2003, 4(2): 142-151.

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author="HE Guo-qing, CHEN Qi-he, ZHANG Li, LIU Xiao-jie",
journal="Journal of Zhejiang University Science A",
volume="4",
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pages="142-151",
year="2003",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2003.0142"
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%A HE Guo-qing
%A CHEN Qi-he
%A ZHANG Li
%A LIU Xiao-jie
%J Journal of Zhejiang University SCIENCE A
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%P 142-151
%@ 1869-1951
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2003.0142

TY - JOUR
T1 - Influence of medium components on elastase production using crude sources by Bacillus sp. EL31410
A1 - HE Guo-qing
A1 - CHEN Qi-he
A1 - ZHANG Li
A1 - LIU Xiao-jie
J0 - Journal of Zhejiang University Science A
VL - 4
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EP - 151
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Y1 - 2003
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2003.0142


Abstract: 
A newly isolated strain EL31410, producing elastase (E.C3.4.4.7) with high elastolytic activity was identified as Bacillus sp. In the medium optimization, it was found that wheat bran and soybean flour hydrosate were the best crude carbon and nitrogen source for enzyme production, respectively. Addition of corn steep flour can affect the bacterium growth and elastase production. A fractional factorial design was applied to study the main factors that affect the enzyme production, and central composite experimental design and response surface methodology were adopted to derive a statistical model for the effect of wheat bran and soybean flour hydrosate on elastase production. The experimental results showed that wheat bran had positive effect but soybean flour hydrosate had negative effect, on enzyme production. An initial concentration of 3.4%(w/v) wheat bran and 9.4%(v/v) soybean flour hydrosate were found to be optimal for enzyme production in batch culture. The time course of elastase production in the optimized medium composition was also described.

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