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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.1 P.77-84


Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119

Author(s):  Dan YE, Zhi-nan XU, Pei-lin CEN

Affiliation(s):  Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   znxu@zju.edu.cn

Key Words:  Cytosine-substituted mildiomycin analogue (MIL-C), Plackett-Burman design, Response surface methodology, Streptoverticillium rimofaciens, Nucleoside antibiotic

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Dan YE, Zhi-nan XU, Pei-lin CEN. Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119[J]. Journal of Zhejiang University Science B, 2008, 9(1): 77-84.

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publisher="Zhejiang University Press & Springer",

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%T Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119
%A Dan YE
%A Zhi-nan XU
%A Pei-lin CEN
%J Journal of Zhejiang University SCIENCE B
%V 9
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%P 77-84
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B071372

T1 - Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119
A1 - Dan YE
A1 - Zhi-nan XU
A1 - Pei-lin CEN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 1
SP - 77
EP - 84
%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B071372

cytosine-substituted mildiomycin analogue (MIL-C) was produced effectively by supplementing cytosine into the culture of Streptoverticillium rimofaciens. In order to improve the yield of MIL-C, statistically-based experimental designs were applied to optimize the fermentation medium for S. rimofaciens ZJU 5119. Fifteen culture conditions were examined for their significances on MIL-C production using plackett-Burman design. The plackett-Burman design and one-variable-at-a-time design indicated that glucose and rice meal as the complex carbon sources, and peanut cake meal and NH4NO3 as the complex nitrogen sources were beneficial for MIL-C production in S. rimofaciens ZJU 5119. The results of further central composition design (CCD) showed that the optimal concentration of glucose, rice meal and peanut cake meal were 18.7 g/L, 64.8 g/L and 65.1 g/L, respectively. By using this optimal fermentation medium, the MIL-C concentration was increased up to 1336.5 mg/L, an approximate 3.8-fold improvement over the previous concentration (350.0 mg/L) with un-optimized medium. This work will be very helpful to the large-scale production of MIL-C in the future.

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