CLC number: TQ9; Q819
On-line Access: 2024-08-27
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Dan YE, Zhi-nan XU, Pei-lin CEN. Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119[J]. Journal of Zhejiang University Science B, 2008, 9(1): 77-84.
@article{title="Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119",
author="Dan YE, Zhi-nan XU, Pei-lin CEN",
journal="Journal of Zhejiang University Science B",
volume="9",
number="1",
pages="77-84",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B071372"
}
%0 Journal Article
%T Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119
%A Dan YE
%A Zhi-nan XU
%A Pei-lin CEN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 1
%P 77-84
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B071372
TY - JOUR
T1 - Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119
A1 - Dan YE
A1 - Zhi-nan XU
A1 - Pei-lin CEN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 1
SP - 77
EP - 84
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B071372
Abstract: cytosine-substituted mildiomycin analogue (MIL-C) was produced effectively by supplementing cytosine into the culture of Streptoverticillium rimofaciens. In order to improve the yield of MIL-C, statistically-based experimental designs were applied to optimize the fermentation medium for S. rimofaciens ZJU 5119. Fifteen culture conditions were examined for their significances on MIL-C production using plackett-Burman design. The plackett-Burman design and one-variable-at-a-time design indicated that glucose and rice meal as the complex carbon sources, and peanut cake meal and NH4NO3 as the complex nitrogen sources were beneficial for MIL-C production in S. rimofaciens ZJU 5119. The results of further central composition design (CCD) showed that the optimal concentration of glucose, rice meal and peanut cake meal were 18.7 g/L, 64.8 g/L and 65.1 g/L, respectively. By using this optimal fermentation medium, the MIL-C concentration was increased up to 1336.5 mg/L, an approximate 3.8-fold improvement over the previous concentration (350.0 mg/L) with un-optimized medium. This work will be very helpful to the large-scale production of MIL-C in the future.
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