Full Text:   <2136>

CLC number: Q344.5

On-line Access: 

Received: 2003-03-04

Revision Accepted: 2003-05-05

Crosschecked: 0000-00-00

Cited: 1

Clicked: 3699

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.2 P.186-192


A large scale screen for genes (3rd chromosome) related to Wingless signaling pathway

Author(s):  LIN Xin-da, LIN Xin-hua, CHENG Jia-an

Affiliation(s):  Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   linxinda@yahoo.com, linxh@uc.edu, jacheng@sun.zju.edu.cn

Key Words:  Drosophila, Genetic screen, Wingless signal transduction, Mutant allele


Share this article to: More

LIN Xin-da, LIN Xin-hua, CHENG Jia-an. A large scale screen for genes (3rd chromosome) related to Wingless signaling pathway[J]. Journal of Zhejiang University Science A, 2004, 5(2): 186-192.

@article{title="A large scale screen for genes (3rd chromosome) related to Wingless signaling pathway",
author="LIN Xin-da, LIN Xin-hua, CHENG Jia-an",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T A large scale screen for genes (3rd chromosome) related to Wingless signaling pathway
%A LIN Xin-da
%A LIN Xin-hua
%A CHENG Jia-an
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 2
%P 186-192
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0186

T1 - A large scale screen for genes (3rd chromosome) related to Wingless signaling pathway
A1 - LIN Xin-da
A1 - LIN Xin-hua
A1 - CHENG Jia-an
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 2
SP - 186
EP - 192
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.0186

A wing specific F1 genetic screen was carried out using the powerfuldrosophila genetic system, combined with yeast FRT/FLP and GAL4/UAS system. Form the wing phenotypes and germline clone embryonic cuticle phenotypes observed in these mutant alleles, a number of mutant alleles of known or unknown genes were isolated. Among them, fifteen mutant alleles related to wingless signal transduction were further isolated; the arm of these mutations located were determined, and their location in the chromosome were roughly mapped.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Bhanot, P., Fish, M., Jemison, J.A., Nusse, R., Nathans, J., Cadigan, K.M., 1999. Frizzled and DFrizzled-2 function as redundant receptors for Wingless duringDrosophilaembryonic development.Development,126:4175-4186.

[2] Brand, A.H., Perrimon, N., 1993. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.Development,118(2):401-415.

[3] Cadigan, K.M., Nusse, R., 1997. Wnt signaling: a common theme in animal development.Genes & Dev,11:3286-3305.

[4] Chou, T.B., Noll, E., Perrimon, N., 1993. AutosomalP[ovoD1] dominant female sterile insertions inDrosophilaand their use in generating germ-line chimeras.Development,119(4):1359-1369.

[5] Chou, T.B., Perrimon, N., 1996. The autosomal FLP-DFStechnique for generating germline mosaics inDrosophila melanogaster.Genetics,144(4):1673-1679.

[6] Duffy, J.B., 2002. GAL4 system inDrosophila: a fly geneticist's Swiss army knife.Genesis,34(1-2):1-15.

[7] Duffy, J.B., Harrison, D.A., Perrimon, N., 1998. Identifying loci required for follicular patterning using directed mosaics.Development,125(12):2263-2271.

[8] Nüsselein-Volhard, C., Wieschaus, E., 1980. Mutations affectiong segment number and polarity in Drosophila.Nature,287:795-801.

[9] Rorth, P., Sazbo, K., Balley, A., Laverty, T., Rehm, J., Rubin, G.M., Weigmann, K., Milan, M., Benes, V., Ansorge, W., Cohen, S.M., 1998. Systematic gain-of-function genetics in Drosophila.Development,126:1049-1057.

[10] Siegfried, E., Perrimon, N., 1994.Drosophilawg: a paradigm for the function and mechanism of Wnt signaling.Bioessays,16:395-404.

[11] Simmonds, A.J., Brook, W.J., Cohen, S.M., Bell, J.B., 1995. Distinguishable functions for engrailed and invected in anterior-posterior patterning in theDrosophilawing.Nature,376:424-427.

[12] Spradling, A.C., Stern, D.M., Kisss, I., Roote, J., Laverty, T., Rubin, G.M., 1995. Gene disruptions using P transposable elements: an integral component of theDrosophilagenome project.Proc Natl Acad Sci USA,92:10824-10830.

[13] van de Wetering, M., Cavallo, R., Dooijes, D., van Beest, M., van Es, J., Loureiro, J., Ypma, A., Hursh, D., Jones, T., Bejsovec, A., Peifer, M., Mortin, M., Clevers, H., 1997. Armadillo coactivates transcription driven by the product of theDrosophilasegment polarity gene dTCF.Cell, 88:789-799.

[14] Wehrli, M., Dougan, S.T., Caldwell, K. O'Keefe, L., Schwartz, S., Vaizel-Ohayon, D., Schejter, E., Tomlinson, A., DiNardo, S., 2000. arrow encodes an LDL-receptor-related protein essential for Wingless signalling.Nature,407:527-530.

[15] Wodarz, A., Nusse, R., 1998. Mechanisms of Wnt signaling in development.Annu Rev Cell Dev Biol,14:59-88.

[16] Xu, T., Rubin, G.M., 1993. Analysis of genetic mosaics in developing and adultDrosophilatissues.Development,117(4):1223-1237.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE