CLC number: TH742.1
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
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WANG Li-qiang, LU Zu-kang. Improving the sensitivity of protein microarray by evanescent-field-induced fluorescence[J]. Journal of Zhejiang University Science A, 2005, 6(7): 623-626.
@article{title="Improving the sensitivity of protein microarray by evanescent-field-induced fluorescence",
author="WANG Li-qiang, LU Zu-kang",
journal="Journal of Zhejiang University Science A",
volume="6",
number="7",
pages="623-626",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.A0623"
}
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%T Improving the sensitivity of protein microarray by evanescent-field-induced fluorescence
%A WANG Li-qiang
%A LU Zu-kang
%J Journal of Zhejiang University SCIENCE A
%V 6
%N 7
%P 623-626
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%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.A0623
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T1 - Improving the sensitivity of protein microarray by evanescent-field-induced fluorescence
A1 - WANG Li-qiang
A1 - LU Zu-kang
J0 - Journal of Zhejiang University Science A
VL - 6
IS - 7
SP - 623
EP - 626
%@ 1673-565X
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.A0623
Abstract: To improve the sensitivity of protein microarray, a prism surface replaces the surface of the common microscope slide. The protein targets arrayed on the surface are hybridized and labelled by fluorescent probes. evanescent excitation occurs when the convergent laser reaches the surface, and a photomultiplier tube detects the emitted fluorescent signal. A two-dimensional actuator scans the whole surface to achieve planar laser excitation and fluorescence collection. The penetration depth of the evanescent field into the protein targets is only some hundred nanometers and can be controlled by different incident angle of the laser beam, so the undesired background signals are reduced dramatically and the detection sensitivity is improved by a factor of 50 to 100 comparing to confocal excitation. This approach can detect low abundance analytes without signal amplification.
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