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Received: 2020-08-11

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Ping XU


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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.7 P.548-562


Potential effect of EGCG on the anti-tumor efficacy of metformin in melanoma cells

Author(s):  Anan XU, Jeehyun LEE, Yueling ZHAO, Yuefei WANG, Xiaoli LI, Ping XU

Affiliation(s):  Department of Tea Science, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   zdxp@zju.edu.cn

Key Words:  Epigallocatechin-3-gallate (EGCG), Metformin, Melanoma, Anti-tumor efficacy

Anan XU, Jeehyun LEE, Yueling ZHAO, Yuefei WANG, Xiaoli LI, Ping XU. Potential effect of EGCG on the anti-tumor efficacy of metformin in melanoma cells[J]. Journal of Zhejiang University Science B, 2021, 22(7): 548-562.

@article{title="Potential effect of EGCG on the anti-tumor efficacy of metformin in melanoma cells",
author="Anan XU, Jeehyun LEE, Yueling ZHAO, Yuefei WANG, Xiaoli LI, Ping XU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Potential effect of EGCG on the anti-tumor efficacy of metformin in melanoma cells
%A Anan XU
%A Jeehyun LEE
%A Yueling ZHAO
%A Yuefei WANG
%A Xiaoli LI
%A Ping XU
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 7
%P 548-562
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000455

T1 - Potential effect of EGCG on the anti-tumor efficacy of metformin in melanoma cells
A1 - Anan XU
A1 - Jeehyun LEE
A1 - Yueling ZHAO
A1 - Yuefei WANG
A1 - Xiaoli LI
A1 - Ping XU
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 7
SP - 548
EP - 562
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000455

metformin, a first-line drug for type 2 diabetes mellitus, has been recognized as a potential anti-tumor agent in recent years. epigallocatechin-3-gallate (EGCG), as the dominant catechin in green tea, is another promising adjuvant agent for tumor prevention. In the present work, the potential effect of EGCG on the anti-tumor efficacy of metformin in a mouse melanoma cell line (B16F10) was investigated. Results indicated that EGCG and metformin exhibited a synergistic effect on cell viability, migration, and proliferation, as well as signal transducer and activator of transcription 3/nuclear factor-κB (STAT3/NF-κB) pathway signaling and the production of inflammation cytokines. Meanwhile, the combination showed an antagonistic effect on cell apoptosis and oxidative stress levels. The combination of EGCG and metformin also differentially affected the nucleus (synergism) and cytoplasm (antagonism) of B16F10 cells. Our findings provide new insight into the potential effects of EGCG on the anti-tumor efficacy of metformin in melanoma cells.


方法:利用CCK8测定细胞增殖,用细胞划痕实验测定细胞运动特性,利用流式细胞仪分析凋亡和细胞周期,利用免疫组织化学染色法观察细胞切片,用酶联免疫吸附试验(ELISA)测定炎症细胞因子和抗氧化酶的蛋白水平含量,用定量逆转录聚合酶链反应(qRT-PCR)检测其基因水平含量,用蛋白质印迹(western blot)探究其可能影响的细胞通路,用拉曼光谱技术从亚细胞水平探究作用机制。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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