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CLC number: TQ041+.8

On-line Access: 2017-01-26

Received: 2015-11-30

Revision Accepted: 2016-04-10

Crosschecked: 2017-01-04

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Sheng-rong Shen


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.2 P.161-171


Effects of astaxanthin on oxidative stress induced by Cu2+ in prostate cells

Author(s):  Hong-zhou Meng, Xiao-feng Ni, Hai-ning Yu, Shan-shan Wang, Sheng-rong Shen

Affiliation(s):  Department of Urology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   shrshen@zju.edu.cn

Key Words:  Oxidative stress, PC-3, RWPE-1, Astaxanthin, Copper ion

Hong-zhou Meng, Xiao-feng Ni, Hai-ning Yu, Shan-shan Wang, Sheng-rong Shen. Effects of astaxanthin on oxidative stress induced by Cu2+ in prostate cells[J]. Journal of Zhejiang University Science B, 2017, 18(2): 161-171.

@article{title="Effects of astaxanthin on oxidative stress induced by Cu2+ in prostate cells",
author="Hong-zhou Meng, Xiao-feng Ni, Hai-ning Yu, Shan-shan Wang, Sheng-rong Shen",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of astaxanthin on oxidative stress induced by Cu2+ in prostate cells
%A Hong-zhou Meng
%A Xiao-feng Ni
%A Hai-ning Yu
%A Shan-shan Wang
%A Sheng-rong Shen
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 2
%P 161-171
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500296

T1 - Effects of astaxanthin on oxidative stress induced by Cu2+ in prostate cells
A1 - Hong-zhou Meng
A1 - Xiao-feng Ni
A1 - Hai-ning Yu
A1 - Shan-shan Wang
A1 - Sheng-rong Shen
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 2
SP - 161
EP - 171
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500296

astaxanthin (AST), a carotenoid molecule extensively found in marine organisms and increasingly used as a dietary supplement, has been reported to have beneficial effects against oxidative stress. In the current paper, the effects of AST on viability of prostate cells were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay; cell apoptosis and intracellular reactive oxygen species (ROS) levels were determined by flow cytometry; the mitochondrial membrane potential (MMP) was measured by fluorospectrophotometer; and activities of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were evaluated by a detection kit. The results show that copper ion (Cu2+) induced apoptosis, along with the accumulation of intracellular ROS and MDA, in both prostate cell lines (RWPE-1 and PC-3). AST treatments could decrease the MDA levels, increase MMP, and keep ROS stable in RWPE-1 cell line. An addition of AST decreased the SOD, GSH-Px, and CAT activities in PC-3 cell line treated with Cu2+, but had a contrary reaction in RWPE-1 cell lines. In conclusion, AST could contribute to protecting RWPE-1 cells against Cu2+-induced injuries but could cause damage to the antioxidant enzyme system in PC-3 cells.




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