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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.6 P.457-463

http://doi.org/10.1631/jzus.2005.B0457


Pathogenicity of bacterium, Xenorhabdus nematophila isolated from entomopathogenic nematode (Steinernema carpocapsae) and its secretion against Galleria mellonella larvae


Author(s):  MAHAR Ali Nawaz, MUNIR Muhammad, ELAWAD Sami, GOWEN Simon Richard, HAGUE Nigel Graham Meckenzi

Affiliation(s):  Department of Agriculture, University of Reading, Reading RG6 6AT, UK; more

Corresponding email(s):   m_munir66@yahoo.co.uk

Key Words:  Biological control, Bacterial symbionts, Entomopathogenic nematodes, Xenorhabdus nematophila, Galleria mellonella


MAHAR Ali Nawaz, MUNIR Muhammad, ELAWAD Sami, GOWEN Simon Richard, HAGUE Nigel Graham Meckenzi. Pathogenicity of bacterium, Xenorhabdus nematophila isolated from entomopathogenic nematode (Steinernema carpocapsae) and its secretion against Galleria mellonella larvae[J]. Journal of Zhejiang University Science B, 2005, 6(6): 457-463.

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author="MAHAR Ali Nawaz, MUNIR Muhammad, ELAWAD Sami, GOWEN Simon Richard, HAGUE Nigel Graham Meckenzi",
journal="Journal of Zhejiang University Science B",
volume="6",
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pages="457-463",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0457"
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%A MAHAR Ali Nawaz
%A MUNIR Muhammad
%A ELAWAD Sami
%A GOWEN Simon Richard
%A HAGUE Nigel Graham Meckenzi
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
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A1 - MAHAR Ali Nawaz
A1 - MUNIR Muhammad
A1 - ELAWAD Sami
A1 - GOWEN Simon Richard
A1 - HAGUE Nigel Graham Meckenzi
J0 - Journal of Zhejiang University Science B
VL - 6
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SP - 457
EP - 463
%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2005.B0457


Abstract: 
The entomopathogenic bacterium, Xenorhabdus nematophila was isolated from the hemolymph of Galleria mellonella infected with Steinernema carpocapsae. The bacterial cells and its metabolic secretions have been found lethal to the Galleria larvae. Toxic secretion in broth caused 95% mortality within 4 d of application whereas the bacterial cells caused 93% mortality after 6 d. When filter and sand substrates were compared, the later one was observed as appropriate. Similarly, bacterial cells and secretion in broth were more effective at 14% moisture and 25 °C temperature treatments. Maximum insect mortality (100%) was observed when bacterial concentration of 4×106 cells/ml was used. Similarly, maximum bacterial cells in broth (95%) were penetrated into the insect body within 2 h of their application. However, when stored bacterial toxic secretion was applied to the insects its efficacy declined. On the other hand, when the same toxic secretion was dried and then dissolved either in broth or water was proved to be effective. The present study showed that the bacterium, X. nematophila or its toxic secretion can be used as an important component of integrated pest management against Galleria.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Akhurst, R.J., Boemare, N.E., 1988. A numerical taxonomic study of the genus Xenorhabdus (Enterobacteriaceae) and proposed elevation of the subspecies of X. nematophilus to species. Journal of General Microbiology, 134:1835-1845.

[2] Akhurst, R.J., Boemare, N.E., 1990. Biology and Taxonomy of Xenorhabdus. In: Gaugler, R., Kaya, H.K. (Eds.), Entomopathogenic Nematodes in Biological Control. C.R.C. Press, Boca Raton, Florida, p.75-90.

[3] Akhurst, R.J., Dunphy, G.B., 1993. Tripartite Interaction between Symbiotically Associated Bacteria (Xenorhabdus spp) and Nematodes (Steinernenatidae and Heterorhabditidae) and Their Insect Hosts. In: Beckage, N., Thomson, S., Federici, B. (Eds.), Entomopathogenic Nematodes in Biological Control. Academic Press, Inc., New York, p.75-87.

[4] Boemare, N.E., Givaudan, A., Brehelin, M., Laumond, C., 1997. Symbiosis and pathogencity of nematode bacterium complexes. Symbiosis, 22:21-45.

[5] Bowen, D.J., Ensign, J.C., 1998. Purification and characterization of the high-molecular-weight insecticidal protein complex produced by the entomopathogenic bacterium, Photorhabdus luminescens. Applied and Environmental Microbiology, 64:3029-3035.

[6] Dudney, R.A., 1997. Use of Xenorhabdus Nematophilus Im/l and 1906/1 for Fire Ant Control. US Patent, No. 5616318.

[7] Elawad, S.A., 1998. Studies on the Taxonomy and Biology of a Newly Isolated Species of Steinernema (Steinernematidae: Nematoda) from the Tropics and Its Associated Bacteria. Ph.D. Thesis, Department of Agriculture, University of Reading, UK.

[8] Elawad, S.A., Gowen, S.R., Hague, N.G.M., 1999. Efficacy of bacterial symbionts from entomopathogenic nematodes against the beet army worm (Spodoptera exigua). Test of Agrochemicals and Cultivars No. 20, Annals of Applied Biology (Supplement), 134:66-67.

[9] Ensign, J.C., Bowen, D.J., Tenor, J.L., Ciche, T.A., Petell, J.K., Strickland, J.A., Orr, G.L., Fatig, R.O., Bintrim, S.B., ffrench-Constant, R.H., 2002. Proteins from the Genus Xenorhabdus are Toxic to Insects on Oral Exposure. US Patent, No. 0147148 A1.

[10] ffrench-Constant, R., Bowen, D., 1999. Photorhabdus toxins: novel biological insecticides. Current Opinion in Microbiology, 2:284-288.

[11] Forst, S., Nealson, K., 1996. Molecular biology of the symbiotic-pathogenic bacteria Xenorhabdus spp. and Photorhabdus spp. Microbiological Reviews, 60:21-43.

[12] Gotz, P., Boman, A., Boman, H.G., 1981. Interactions between insect immunity and an insect-pathogenic nematode with symbiotic bacteria. Proceedings of Royal Society London, 212:333-350.

[13] Kaya, H.K., Gaugler, R., 1993. Entomopathogenic nematodes. Annual Review of Entomology, 38:181-206.

[14] Mahar, A.N., 2003. The Efficacy of Bacteria Isolated from Entomopathogenic Nematodes against the Diamondback Moth Plutella xylostella L. (Lepidoptera: Yponomeutidae). Ph.D. Thesis, Department of Agriculture, University of Reading, UK.

[15] Mohan, S., Raman, R., Gaur, H.S., 2003. Foliar application of Photorhabdus luminescens, symbiotic bacteria from entomopathogenic nematode Heterorhabditis indica, to kill cabbage butterfly Pieris brassicae. Current Science, 84:1397.

[16] Poinar, Jr.G.O., 1979. Nematodes for Biological Control of Insects. C.R.C. Press, Boca Raton, Florida.

[17] Poinar, Jr.G.O., Thomas, G.M., 1966. Significance of Achromobacter nematophilus Poinar and Thomas (Achromobactereace: Eubacteriale), in the development of the nematode, DD-136 (Neoplectanta sp., Steinernematidae). Parasitology, 56:385-388.

[18] Samaliev, H.Y., Andreoglou, F.I., Elawad, S.A., Hague, N.G.M., Gowen, S.R., 2000. The nematicidal effects of the bacteria Pseudomonas oryzihabitans and Xenorhabdus nematophilus on the root-knot nematode Meloidogyne javanica. Nematology, 2:507-514.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Dr. Mohite@Agriculture College<pandurang6@yahoo.co.in>

2014-11-07 17:43:40

This will be reference to conduct PG Research to my PG student

REVATHI@STUDENT<revs.agri@gmail.com>

2012-02-07 18:01:41

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