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Received: 2005-02-23

Revision Accepted: 2005-05-03

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.7 P.650-655


Influence of CO2 pneumoperitoneum on intracellular pH and signal transduction in cancer cells

Author(s):  CAO Li-ping, DING Guo-ping, QUE Ri-sheng, ZHENG Shu

Affiliation(s):  Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   cao@zju.edu.cn

Key Words:  Cancer, CO2 pneumoperitoneum, Intracellular pH, Signal transduction

CAO Li-ping, DING Guo-ping, QUE Ri-sheng, ZHENG Shu. Influence of CO2 pneumoperitoneum on intracellular pH and signal transduction in cancer cells[J]. Journal of Zhejiang University Science B, 2005, 6(7): 650-655.

@article{title="Influence of CO2 pneumoperitoneum on intracellular pH and signal transduction in cancer cells",
author="CAO Li-ping, DING Guo-ping, QUE Ri-sheng, ZHENG Shu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Influence of CO2 pneumoperitoneum on intracellular pH and signal transduction in cancer cells
%A CAO Li-ping
%A DING Guo-ping
%A QUE Ri-sheng
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 7
%P 650-655
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0650

T1 - Influence of CO2 pneumoperitoneum on intracellular pH and signal transduction in cancer cells
A1 - CAO Li-ping
A1 - DING Guo-ping
A1 - QUE Ri-sheng
A1 - ZHENG Shu
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 7
SP - 650
EP - 655
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0650

Object: The authors studied the influence of CO2 pneumoperitoneum on intracellular pH and signal transduction arising from cancer cell multiplication in laparoscopic tumor operation. Method: They set up a simulation of pneumoperitoneum under different CO2 pressure, and then measured the variation of intracellular pH (pHi) at different time and the activity of protein kinase C (PKC) and protein phosphatase 2a (PP2a) at the end of the pneumoperitoneum. After 1 week, the concentration of cancer cells in the culture medium was calculated. Result: When the pressure of CO2 pneumoperitoneum was 0, 10, 20, 30 mmHg respectively, the average pHi was 7.273, 7.075, 6.783, 6.693 at the end of the pneumoperitoneum; PKC activity was 159.4, 168.5, 178.0, 181.6 nmol/(g·min) and PP2a was 4158.3, 4066.9, 3984.0, 3878.5 nmol/(g·min) respectively. After 1 week, the cancer cells concentration was 2.15×105, 2.03×105, 2.20×105, 2.18×105 L−1. Conclusion: CO2 pneumoperitoneum could promote acidosis in cancer cells, inducing the activation of protein kinase C and deactivation of protein phosphatase 2a, but it could not accelerate the mitosis rate of the cancer cells.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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