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Abstract: Objective: To observe the effect of yangxueqingnao particles on rat vascular smooth muscle cell (VSMC) proliferation induced by lysophosphatidic acid (LPA). Methods: The amount of ³H-TdR (³H-thymidine) admixed in cultured rat VSMC was measured and mitogen-activated protein kinase (MAPK) activity and lipid peroxidation end product malondialdehyde (MDA) content of the VSMC were assayed. Results: 1×10⁻⁹, 1×10⁻⁸, 1×10⁻⁷ mol/L LPA in a concentration dependent manner, induced the amount of ³H-TdR admixed, MAP kinase activity, and MDA content of the cultured rat VSMC to increase. However, 5%, 10%, and 15% Yangxueqingnao serum preincubation resulted in a decrease of 23.0%, 42.0%, and 52.0% (P<0.01) respectively in the amount of ³H-TdR admixed, a decline in VSMC MAP kinase activity of 13.9% (P<0.05), 29.6% (P<0.01), and 48.9% (P<0.01) respectively, and also, a decrease in MDA content of VSMC of 19.4%, 24.7%, and 43.2% (P<0.01) respectively, in the 1×10⁻⁷ mol/L LPA-treated VSMC. Conclusions: LPA activates the proliferation and lipid peroxidation of VSMC in a concentration dependent manner. The LPA-induced VSMC proliferation is related to the activity of MAP kinases, enzymes involved in an intracellular signalling pathway. The results of the present study showed that yangxueqingnao particles can effectively inhibit LPA-induced VSMC proliferation, MAP kinase activation, and reduce lipid peroxidative lesion.

[1] Fueller, M., Wang cle, A., Tiggi, G., Siess, W., 2003. Activation of human monocytic cells by lysophosphatidic acid and sphingosine-1-phosphate. Cell Signal, 15:367-375.

[2] Gennero, I., Xuereb, J.M., Simon, M.F., Girolami, J.P., Bascands, J.L., Chap, H., Boneu, B., Sie, P., 1999. Effects of lysophosphatidic acid on proliferation and cytosolic Ca²⁺ of human adult vascular smooth muscle cells in culture. Thromb. Res., 94:317-326.

[3] Hayashi, K., Takahashi, M., Nishda, W., Yoshida, K., Ohakawa, Y., Kitabatake, A., Aoki, J., Arai, H., Sobue, K., 2001. Phenotypic modulation of vascular smooth muscle cells induced by unsaturated lysophosphatidic acids. Circ. Res., 89:251-258.

[4] Li, Y.K., WU, J.Y., 1999. The discussion of “ordinary method” of obtaining serum in serum pharmacology method. Chinese Pharmacological Bulletin, 15:569-570.

[5] Niu, D.D., Shi, Y.R., Jiang, H.F., Sang, Y.T., Pang, Y.Z., Wu, Q.Z., 2002. Tuarine inhibits rat vascular smooth muscle cell proliferation induced by lysophosphatidic acid. Chinese Pharmacological Bulletin, 18:275-278.

[6] Niu, J.P., Xue, P., Wu, Q.Z.H., Li, D., Yao, C.S., Wang, Y.H., 2003. Experimental studies of relationship between phospholipid level and Yangxueqingnao particles in transient cerebral ischemia attack. Chinese Journal of Clinical Rehabilitation, 7:500-501 (in Chinese).

[7] Ren, Y., Pan, C., Wu, Q., 2003. Involvement of endothelin in the proliferative effect of lysophosphatidic acid on vascular smooth muscle cells in rats. Journal of Beijing University, 35:508-511 (in Chinese).

[8] Seewald, S., Sachinidis, A., Dusing, R., Ko, Y., Seul, C., 1997. Lysophosphatidic acid and intracellular signalling in vascular smooth muscle cells. Atherosclerosis, 130:121-131.