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Received: 2005-07-19

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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.2 P.107-113


Changes of CREB in rat hippocampus, prefrontal cortex and nucleus accumbens during three phases of morphine induced conditioned place preference in rats

Author(s):  Zhou Lian-fang, Zhu Yong-ping

Affiliation(s):  Department of Toxicology, School of Medicine, Zhejiang University, Hangzhou 310006, China; more

Corresponding email(s):   zhoulianfang@163.com, zhuyp@zju.edu.cn

Key Words:  Conditioned place preference (CPP), Morphine, Video tracking, CREB (cAMP response element binding protein)

Zhou Lian-fang, Zhu Yong-ping. Changes of CREB in rat hippocampus, prefrontal cortex and nucleus accumbens during three phases of morphine induced conditioned place preference in rats[J]. Journal of Zhejiang University Science B, 2006, 7(2): 107-113.

@article{title="Changes of CREB in rat hippocampus, prefrontal cortex and nucleus accumbens during three phases of morphine induced conditioned place preference in rats",
author="Zhou Lian-fang, Zhu Yong-ping",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Changes of CREB in rat hippocampus, prefrontal cortex and nucleus accumbens during three phases of morphine induced conditioned place preference in rats
%A Zhou Lian-fang
%A Zhu Yong-ping
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 2
%P 107-113
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0107

T1 - Changes of CREB in rat hippocampus, prefrontal cortex and nucleus accumbens during three phases of morphine induced conditioned place preference in rats
A1 - Zhou Lian-fang
A1 - Zhu Yong-ping
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 2
SP - 107
EP - 113
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0107

Objective: To investigate the changes in CREB (cAMP response element binding protein) in hippocampus, PFC (prefrontal cortex) and NAc (nucleus accumbens) during three phases of morphine induced CPP (conditioned place preference) in rats, and to elucidate the role of CREB during the progress of conditioned place preference. Methods: morphine induced CPP acquisition, extinction and drug primed reinstatement model was established, and CREB expression in each brain area was measured by Western Blot methods. Results: Eight alternating injections of morphine (10 mg/kg) induced CPP, and 8 d saline extinction training that extinguished CPP. CPP was reinstated following a priming injection of morphine (2.5 mg/kg). During the phases of CPP acquisition and reinstatement, the level of CREB expression was significantly changed in different brain areas. Conclusion: It was proved that CPP model can be used as an effective tool to investigate the mechanisms underlying drug-induced reinstatement of drug seeking after extinction, and that morphine induced CPP and drug primed reinstatement may involve activation of the transcription factor CREB in several brain areas, suggesting that the CREB and its target gene regulation pathway may mediate the basic mechanism underlying opioid dependence and its drug seeking behavior.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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