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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.11 P.892-898


Chronic morphine drinking establishes morphine tolerance, but not addiction in Wistar rats

Author(s):  BINSACK Ralf, ZHENG Ming-lan, ZHANG Zhan-sai, YANG Liu, ZHU Yong-ping

Affiliation(s):  Department of Toxicology, School of Medicine, Zhejiang University, Hangzhou 310006, China; more

Corresponding email(s):   zhuyp@zju.edu.cn

Key Words:  Addiction, Morphine, Self-administration, Tolerance, Wistar rats

BINSACK Ralf, ZHENG Ming-lan, ZHANG Zhan-sai, YANG Liu, ZHU Yong-ping. Chronic morphine drinking establishes morphine tolerance, but not addiction in Wistar rats[J]. Journal of Zhejiang University Science B, 2006, 7(11): 892-898.

@article{title="Chronic morphine drinking establishes morphine tolerance, but not addiction in Wistar rats",
author="BINSACK Ralf, ZHENG Ming-lan, ZHANG Zhan-sai, YANG Liu, ZHU Yong-ping",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Chronic morphine drinking establishes morphine tolerance, but not addiction in Wistar rats
%A ZHENG Ming-lan
%A ZHANG Zhan-sai
%A ZHU Yong-ping
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 11
%P 892-898
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0892

T1 - Chronic morphine drinking establishes morphine tolerance, but not addiction in Wistar rats
A1 - ZHENG Ming-lan
A1 - ZHANG Zhan-sai
A1 - YANG Liu
A1 - ZHU Yong-ping
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 11
SP - 892
EP - 898
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0892

Objective: Some animal models apply morphine in the drinking water to generate addiction, but related reports are not free of conflicting results. Accordingly, this study aimed to figure out if chronic consumption of morphine in the drinking water can induce morphine addiction in wistar rats. Methods: For 3 weeks, the animals received a daily morphine dose of 35 mg/kg by offering a calculated volume of sugar water (5% sucrose) with morphine (0.1 mg/ml) to each rat; animals receiving just sugar water served as controls. Immediately after the treatment phase, the tail immersion test was used to check for morphine tolerance, and all animals were then kept on tap water for one week (withdrawal phase). Afterwards, all rats were allowed to choose their drinking source by offering two bottles, containing sugar water without and with morphine, simultaneously for two days (preference phase). Results: While the chronic consumption of morphine led to a reduction in body weight and to morphine tolerance, the morphine-treated wistar rats did not show any preference for the opiate-containing sugar water. Conclusion: Body weight loss and tolerance do not reveal a condition of drug craving, and current animal models should be re-evaluated regarding their potential to establish morphine addicted animals.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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