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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.4 P.291-298

http://doi.org/10.1631/jzus.2006.B0291


Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)


Author(s):  Rao Abdul Qayyum, Hussain S. Sarfraz, Shahzad M. Saqib, Bokhari S. Yassir Abbas, Raza M. Hashim, Rakha Allah, Majeed A., Shahid A. Ali, Saleem Zafar, Husnain Tayyab, Riazuddin S.

Affiliation(s):  Institution Centre of Excellence in Molecular Biology, University of the Punjab, Lahore 53700, Pakistan

Corresponding email(s):   yassir877@yahoo.com

Key Words:  Cotton, Callus, Somatic embryogenic, Wild species, Cell suspension culture


Rao Abdul Qayyum, Hussain S. Sarfraz, Shahzad M. Saqib, Bokhari S. Yassir Abbas, Raza M. Hashim, Rakha Allah, Majeed A., Shahid A. Ali, Saleem Zafar, Husnain Tayyab, Riazuddin S.. Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)[J]. Journal of Zhejiang University Science B, 2006, 7(4): 291-298.

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author="Rao Abdul Qayyum, Hussain S. Sarfraz, Shahzad M. Saqib, Bokhari S. Yassir Abbas, Raza M. Hashim, Rakha Allah, Majeed A., Shahid A. Ali, Saleem Zafar, Husnain Tayyab, Riazuddin S.",
journal="Journal of Zhejiang University Science B",
volume="7",
number="4",
pages="291-298",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0291"
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%0 Journal Article
%T Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)
%A Rao Abdul Qayyum
%A Hussain S. Sarfraz
%A Shahzad M. Saqib
%A Bokhari S. Yassir Abbas
%A Raza M. Hashim
%A Rakha Allah
%A Majeed A.
%A Shahid A. Ali
%A Saleem Zafar
%A Husnain Tayyab
%A Riazuddin S.
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 4
%P 291-298
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0291

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T1 - Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)
A1 - Rao Abdul Qayyum
A1 - Hussain S. Sarfraz
A1 - Shahzad M. Saqib
A1 - Bokhari S. Yassir Abbas
A1 - Raza M. Hashim
A1 - Rakha Allah
A1 - Majeed A.
A1 - Shahid A. Ali
A1 - Saleem Zafar
A1 - Husnain Tayyab
A1 - Riazuddin S.
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 4
SP - 291
EP - 298
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0291


Abstract: 
Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypocotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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