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Received: 2006-02-21

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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.11 P.906-911

http://doi.org/10.1631/jzus.2006.B0906


Receptor-mediated gene delivery using polyethylenimine (PEI) coupled with polypeptides targeting FGF receptors on cells surface


Author(s):  LI Da, WANG Qing-qing, TANG Gu-ping, HUANG Hong-liang, SHEN Fen-ping, LI Jing-zhong, YU Hai

Affiliation(s):  Institute of Immunology, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   yuhai@zju.edu.cn

Key Words:  FGF receptors, Polyethylenimine, Targeting, Gene delivery, Gene therapy


LI Da, WANG Qing-qing, TANG Gu-ping, HUANG Hong-liang, SHEN Fen-ping, LI Jing-zhong, YU Hai. Receptor-mediated gene delivery using polyethylenimine (PEI) coupled with polypeptides targeting FGF receptors on cells surface[J]. Journal of Zhejiang University Science B, 2006, 7(11): 906-911.

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author="LI Da, WANG Qing-qing, TANG Gu-ping, HUANG Hong-liang, SHEN Fen-ping, LI Jing-zhong, YU Hai",
journal="Journal of Zhejiang University Science B",
volume="7",
number="11",
pages="906-911",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0906"
}

%0 Journal Article
%T Receptor-mediated gene delivery using polyethylenimine (PEI) coupled with polypeptides targeting FGF receptors on cells surface
%A LI Da
%A WANG Qing-qing
%A TANG Gu-ping
%A HUANG Hong-liang
%A SHEN Fen-ping
%A LI Jing-zhong
%A YU Hai
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 11
%P 906-911
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0906

TY - JOUR
T1 - Receptor-mediated gene delivery using polyethylenimine (PEI) coupled with polypeptides targeting FGF receptors on cells surface
A1 - LI Da
A1 - WANG Qing-qing
A1 - TANG Gu-ping
A1 - HUANG Hong-liang
A1 - SHEN Fen-ping
A1 - LI Jing-zhong
A1 - YU Hai
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 11
SP - 906
EP - 911
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0906


Abstract: 
Objective: To construct a novel kind of nonviral gene delivery vector based on polyethylenimine (PEI) conjugated with polypeptides derived from ligand FGF with high transfection efficiency and according to tumor targeting ability. Methods: The synthetic polypeptides CR16 for binding FGF receptors was conjugated to PEI and the characters of the polypeptides including DNA condensing and particle size were determined. Enhanced efficiency and the targeting specificity of the synthesized vector were investigated in vitro and in vivo. Results: The polypeptides were successfully coupled to PEI. The new vectors PEI-CR16 could efficiently condense pDNA into particles with around 200 nm diameter. The PEI-CR16/pDNA polyplexes showed significantly greater transgene activity than PEI/pDNA in FGF receptors positive tumor cells in vitro and in vivo gene transfer, while no difference was observed in FGF receptors negative tumor cells. The enhanced transfection efficiency of PEI-CR16 could be blocked by excess free polypeptides. Conclusion: The synthesized vector could improve the efficiency of gene transfer and targeting specificity in FGF receptors positive cells. The vector had good prospect for use in cancer gene therapy.

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Reference

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