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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.4 P.237-241

http://doi.org/10.1631/jzus.2007.B0237


Correlation of serum arylesterase activity on phenylacetate estimated by the integrated method to common classical biochemical indexes of liver damage


Author(s):  LIAO Fei, ZHU Xiao-yun, WANG Yong-mei, ZHAO Yun-sheng, ZHU Lian-ping, ZUO Yu-ping

Affiliation(s):  Laboratory of Bioinformatics & Molecular Engineering, Chongqing Key Laboratory of Biochemistry & Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University, Chongqing 400016, China; more

Corresponding email(s):   liaofeish@yahoo.com, liaofeish@vip.sina.com

Key Words:  Arylesterase, Albumin, Alanine aminotransferase, Liver damage, Ratio of albumin to globulins, Integrated method, Bilirubin


LIAO Fei, ZHU Xiao-yun, WANG Yong-mei, ZHAO Yun-sheng, ZHU Lian-ping, ZUO Yu-ping. Correlation of serum arylesterase activity on phenylacetate estimated by the integrated method to common classical biochemical indexes of liver damage[J]. Journal of Zhejiang University Science B, 2007, 8(4): 237-241.

@article{title="Correlation of serum arylesterase activity on phenylacetate estimated by the integrated method to common classical biochemical indexes of liver damage",
author="LIAO Fei, ZHU Xiao-yun, WANG Yong-mei, ZHAO Yun-sheng, ZHU Lian-ping, ZUO Yu-ping",
journal="Journal of Zhejiang University Science B",
volume="8",
number="4",
pages="237-241",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0237"
}

%0 Journal Article
%T Correlation of serum arylesterase activity on phenylacetate estimated by the integrated method to common classical biochemical indexes of liver damage
%A LIAO Fei
%A ZHU Xiao-yun
%A WANG Yong-mei
%A ZHAO Yun-sheng
%A ZHU Lian-ping
%A ZUO Yu-ping
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 4
%P 237-241
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0237

TY - JOUR
T1 - Correlation of serum arylesterase activity on phenylacetate estimated by the integrated method to common classical biochemical indexes of liver damage
A1 - LIAO Fei
A1 - ZHU Xiao-yun
A1 - WANG Yong-mei
A1 - ZHAO Yun-sheng
A1 - ZHU Lian-ping
A1 - ZUO Yu-ping
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 4
SP - 237
EP - 241
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0237


Abstract: 
The correlation of serum arylesterase (PON1) activity on phenylacetate determined by an integrated method to classical biochemical indexes of liver damage was investigated for the use of PON1 activity to evaluate liver damage. PON1 reaction curve as absorbance at 270 nm for 0.20 mmol/L phenylacetate hydrolysis was analyzed by the integrated method to determine maximal PON1 reaction rate. Classical biochemical indexes of liver damage were determined routinely. The 95% confidence threshold of PON1 activity in sera from healthy individuals was 2.12 mkat/L [(4.73±1.31) mkat/L, n=105]. PON1 activity in clinical sera was closely correlated to serum albumin, total protein and the ratio of albumin to globulins, but was weakly correlated to both direct and total bilirubin in serum. There were no correlations of PON1 activity to γ-glutamyltransferase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase. Among 127 clinical sera with PON1 activity>2.12 mkat/L, there were 92% healthy individuals examined by albumin, 90% healthy individuals examined by total protein, 88% healthy individuals examined by total bilirubin, 86% healthy individuals examined by direct bilirubin and 64% healthy individuals examined by the ratio of albumin to globulins, respectively. In each group of healthy individuals judged by classical biochemical indexes of close correlation to PON1 activity, percentage of healthy individuals examined by PON1 activity was always >80%. These results suggested PON1 activity on phenylacetate estimated by the integrated method was also suitable for the evaluation of liver damage.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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