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CLC number: S816.3

On-line Access: 2015-06-08

Received: 2014-12-01

Revision Accepted: 2015-05-21

Crosschecked: 2015-05-25

Cited: 7

Clicked: 5141

Citations:  Bibtex RefMan EndNote GB/T7714


Wei-fen Li


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.6 P.487-495


Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice

Author(s):  Kai Lei, Ya-li Li, Yang Wang, Jing Wen, Hong-zhao Wu, Dong-you Yu, Wei-fen Li

Affiliation(s):  Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   wfli@zju.edu.cn

Key Words:  Bacillus subtilis, High-fat diet, Oxidative stress, Lipid metabolism

Kai Lei, Ya-li Li, Yang Wang, Jing Wen, Hong-zhao Wu, Dong-you Yu, Wei-fen Li. Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice[J]. Journal of Zhejiang University Science B, 2015, 16(6): 487-495.

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author="Kai Lei, Ya-li Li, Yang Wang, Jing Wen, Hong-zhao Wu, Dong-you Yu, Wei-fen Li",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice
%A Kai Lei
%A Ya-li Li
%A Yang Wang
%A Jing Wen
%A Hong-zhao Wu
%A Dong-you Yu
%A Wei-fen Li
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 6
%P 487-495
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400342

T1 - Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice
A1 - Kai Lei
A1 - Ya-li Li
A1 - Yang Wang
A1 - Jing Wen
A1 - Hong-zhao Wu
A1 - Dong-you Yu
A1 - Wei-fen Li
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 6
SP - 487
EP - 495
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400342

While a high-fat diet (HFD) is assumed to be related to fat-mediated oxidative stress decreasing antioxidant enzyme activity, probiotics are believed to have positive effects on the regulation of HFD-induced obesity as well as lipid metabolism, energy homeostasis, and anti-oxidation. Because Bacillus subtilis B10 has beneficial effects on the abnormal lipid metabolism and the oxidative stress in HFD-induced obese mice, ICR mice were randomly assigned into an HFD group and the HFD was supplemented with 0.1% (w/w) Bacillus subtilis B10 (HFD+B10 group). Thereafter, 30-d treatments were run, and then hepatic lipid level and antioxidant status were measured. The expression of genes related to lipid metabolism and oxidative stress in the liver was determined by reverse-transcription quantitative polymerase chain reaction (RT-qPCR). We found that HFD-induced obese mice treated with B10 showed a decrease in weight gain, serum glucose activity as well as hepatic triglyceride (TG), glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) activities. In addition, the gene expressions of antioxidant genes, glutathione reductase (GR), xanthine oxidase (XO), heat-shock protein 90 (Hsp90), and lipid synthesis gene 3β-hydroxysteroid-∆24 reductase (DHCR24) in the HFD+B10 group were down-regulated, suggesting alleviation of oxidative stress, while the lipolysis gene 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), energy metabolism gene peroxisome proliferator-activated receptor α (PPARα) and the gene encoding tumor-suppressor protein p53 were up-regulated. The regulatory and positive effect of dietary supplementation of probiotic B10 suggests that it has a beneficial effect on the homeostasis of the lipid metabolism and on alleviating oxidative stress in HFD-induced obese mice.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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