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Received: 2006-09-19

Revision Accepted: 2007-01-25

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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.5 P.331-337


Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS)

Author(s):  RAJPUT Zahid Iqbal, XIAO Chen-wen, HU Song-hua, ARIJO Abdullah G., SOOMRO Noor Mohammad

Affiliation(s):  Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   rao_rajput2002@yahoo.com, songhua@zju.edu.cn

Key Words:  Cochinchina momordica, ECMS, Adjuvant, Chicken, Avian influenza (H5N1), Bird flue

RAJPUT Zahid Iqbal, XIAO Chen-wen, HU Song-hua, ARIJO Abdullah G., SOOMRO Noor Mohammad. Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS)[J]. Journal of Zhejiang University Science B, 2007, 8(5): 331-337.

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author="RAJPUT Zahid Iqbal, XIAO Chen-wen, HU Song-hua, ARIJO Abdullah G., SOOMRO Noor Mohammad",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS)
%A RAJPUT Zahid Iqbal
%A XIAO Chen-wen
%A HU Song-hua
%A ARIJO Abdullah G.
%A SOOMRO Noor Mohammad
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 5
%P 331-337
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0331

T1 - Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS)
A1 - RAJPUT Zahid Iqbal
A1 - XIAO Chen-wen
A1 - HU Song-hua
A1 - ARIJO Abdullah G.
A1 - SOOMRO Noor Mohammad
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 5
SP - 331
EP - 337
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0331

Seeds of a Chinese traditional medicine plant, Cochinchina momordica were used in the present study for the improvement of influenza vaccine (H5N1) in chicken. Crude extraction from Cochinchina momordica seed (ECMS) was obtained by ethanol extraction method. In experiment No. 1, two weeks old chickens were immunized with influenza vaccine (H5N1) alone or combined with ECMS (5, 10, 20, 40 and 80 μg/dose). Serum IgG antibody levels (by ELISA) as well as effects on daily weight gain were measured on 0, 7, 14 and 28th day after immunization. Results revealed that all ECMS groups numerically increased the antibody levels while 10 and 20 μg/dose groups significantly (P<0.05) enhanced total IgG antibody on day 28, when compared with control. Average daily weight gain was also significantly higher in 20 μg/dose ECMS group. adjuvant effect was also confirmed in experiment No. 2 when chickens were immunized with 20 μg/dose ECMS and antibody titer was measured through hemagglutination inhibition (HI). It is concluded that ECMS has potential to improve the immune responses and deserve further study as an adjuvant.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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