Full Text:   <2661>

CLC number: R917; O657.1

On-line Access: 2012-01-18

Received: 2011-03-10

Revision Accepted: 2011-07-26

Crosschecked: 2011-12-10

Cited: 2

Clicked: 5073

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.2 P.118-125

http://doi.org/10.1631/jzus.B1100073


Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine


Author(s):  Jian Yi, Meng Meng, Zhong-qiu Liu, Jin-fang Zhi, Yuan-yang Zhang, Jing Xu, Ya-bin Wang, Jin-ting Liu, Ri-mo Xi

Affiliation(s):  School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China; more

Corresponding email(s):   jintliu@sdu.edu.cn, xirimo2000@yahoo.com

Key Words:  Gatifloxacin, Drug residue, Antibody, Carbon fiber electrode, Electrochemical immunoassay


Jian Yi, Meng Meng, Zhong-qiu Liu, Jin-fang Zhi, Yuan-yang Zhang, Jing Xu, Ya-bin Wang, Jin-ting Liu, Ri-mo Xi. Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine[J]. Journal of Zhejiang University Science B, 2012, 13(2): 118-125.

@article{title="Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine",
author="Jian Yi, Meng Meng, Zhong-qiu Liu, Jin-fang Zhi, Yuan-yang Zhang, Jing Xu, Ya-bin Wang, Jin-ting Liu, Ri-mo Xi",
journal="Journal of Zhejiang University Science B",
volume="13",
number="2",
pages="118-125",
year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100073"
}

%0 Journal Article
%T Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine
%A Jian Yi
%A Meng Meng
%A Zhong-qiu Liu
%A Jin-fang Zhi
%A Yuan-yang Zhang
%A Jing Xu
%A Ya-bin Wang
%A Jin-ting Liu
%A Ri-mo Xi
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 2
%P 118-125
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100073

TY - JOUR
T1 - Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine
A1 - Jian Yi
A1 - Meng Meng
A1 - Zhong-qiu Liu
A1 - Jin-fang Zhi
A1 - Yuan-yang Zhang
A1 - Jing Xu
A1 - Ya-bin Wang
A1 - Jin-ting Liu
A1 - Ri-mo Xi
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 2
SP - 118
EP - 125
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100073


Abstract: 
To detect gatifloxacin (GAT) residue in swine urine, an electrochemical immunoassay was established. An indirect competitive immunoassay was developed, in which the coating antigen is immobilized in an enzyme-linked immunosorbent assay (ELISA) plate and GAT residue from the sample competes with the limited binding sites in added anti-GAT antibody. Horseradish peroxidase (HRP) conjugated to goat anti-rabbit IgG was used as the enzymatic label. A carbon fiber working electrode was constructed and current signals were detected by using hydrogen peroxide as a substrate and hydroquinone as an electrochemical mediator. The electrochemical immunoassay was evaluated by analysis of GAT in buffer or swine urine and an average value of half inhibition concentration (IC50) of 8.9 ng/ml was obtained. Excellent specificity of the antibody was achieved with little cross-reaction with lomefloxacin (3.0%), ciprofloxacin (3.0%), and ofloxacin (1.9%) among commonly used (fluoro)quinolones. In conclusion, the immunoassay system developed in this research can be used as a rapid, powerful and on-site analytical tool to detect GAT residue in foods and food products.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Amin, A.S., Gouda, A.A.E.F., El-Sheikh, R., Zahran, F., 2007. Spectrophotometric determination of gatifloxacin in pure form and in pharmaceutical formulation. Spectrochim. Acta A, 67(5):1306-1312.

[2]Bhasin, R., Arce, F.C., Pasmantier, R., 2005. Hypoglycemia associated with the use of gatifloxacin. Am. J. Med. Sci., 330(5):250-253.

[3]Blanco, M.H., Quintana, L.H., Hernandez, L., 1999. Determination of dihydrozeatin riboside in apples by anodic stripping voltammetry with a carbon fiber microelectrode. Fresenius J. Anal. Chem., 364(3):254-260.

[4]Blondeau, J.M., Laskowski, R., Bjarnason, J., Stewart, C., 2000. Comparative in vitro activity of gatifloxacin, grepafloxacin, levofloxacin, moxifloxacin and trovafloxacin against Gram-negative and Gram-positive organisms. Int. J. Antimicrob. Agents, 14(1):45-50.

[5]Conneely, G., O′Mahony, D., Lu, H., Guilbault, G.G., Pravda, M., Aherne, M., 2007. An immunosensor for the detection of stanozolol in bovine urine. Anal. Lett., 40(7):1280-1293.

[6]Espinosa-Mansilla, A., de la Pena, A.M., Gomez, D.G., Lopez, F.S., 2006. Determination of fluoroquinolones in urine and serum by using high performance liquid chromatography and multiemission scan fluorimetric detection. Talanta, 68(4):1215-1221.

[7]Fierens, C., Hillaert, S., van den Bossche, W., 2000. The qualitative and quantitative determination of quinolones of first and second generation by capillary electrophoresis. J. Pharmaceut. Biomed. Anal., 22(5):763-772.

[8]He, Z.H., Gao, N., Jin, W.R., 2003. Capillary electrophoretic enzyme immunoassay with electrochemical detection using a noncompetitive format. J. Chromatogr. B, 784(2):343-350.

[9]Hervás, M., López, M.A., Escarpa, A., 2009. Electrochemical immunoassay using magnetic beads for the determination of zearalenone in baby food: an anticipated analytical tool for food safety. Anal. Chim. Acta, 653(2):167-172.

[10]Hooper, D.C., 1998. Clinical applications of quinolones. BBA-Gene Struct. Expr., 1400(1-3):45-61.

[11]Huang, W.H., Pang, D.W., Tong, H., Wang, Z.L., Cheng, J.K., 2001. A method for the fabrication of low-noise carbon fiber nanoelectrdes. Anal. Chem., 73(5):1048-1052.

[12]Jamal, M., Crowe, M.A., Magner, E., 2005. Characterization of the composition of bovine urine and its effect on the electrochemical analysis of the model mediator, p-aminophenol. Anal. Chim. Acta, 554(1-2):79-85.

[13]Jin, W.R., Li, X.J., Gao, N., 2003. Simultaneous determination of tryptophan and glutathione in individual rat hepatocytes by capillary zone electrophoresis with electrochemical detection at a carbon fiber bundle-Au/Hg dual electrode. Anal. Chem., 75(15):3859-3864.

[14]Kays, M.B., Overholser, B.R., Lagvankar, S., Goldman, M., Sowinski, K.M., 2005. Effect of ensure on the oral bioavailability of gatifloxacin in healthy volunteers. Pharmacotherapy, 25(11):1530-1535.

[15]Laschi, S., Mascini, M., Scortichini, G., Franek, M., Mascini, M., 2003. Polychlorinated biphenyls (PCBs) detection in food samples using an electrochemical immunosensor. J. Agric. Food Chem., 51(7):1816-1822.

[16]Lei, C.X., Hu, S.Q., Gao, N., Shen, G.L., Yu, R.Q., 2004. An amperometric hydrogen peroxide biosensor based on immobilizing horseradish peroxidase to a nano-Au monolayer supported by sol-gel derived carbon ceramic electrode. Bioelectrochemistry, 65(1):33-39.

[17]Liu, Y., Zhang, C.Z., Yu, X.Y., Zhang, Z.Y., Zhang, X., Liu, R.R., Liu, X.J., Gong, Z.M., 2007. Development and evaluation of immunoassay for zeranol in bovine urine. J. Zhejiang Univ.-Sci. B, 8(12):900-905.

[18]Lu, S.X., Zhang, Y.L., Liu, J.T., Zhao, C.B., Liu, W., Xi, R.M., 2006. Preparation of anti-pefloxacin antibody and development of an indirect competitive enzyme-linked immunosorbent assay for detection of pefloxacin residue in chicken liver. J. Agric. Food Chem., 54(19):6995-7000.

[19]Netchiporouk, L., Shram, N., Salvert, D., Cespuglio, R., 2001. Brain extracellular glucose assessed by voltammetry throughout the rat sleep-wake cycle. Eur. J. Neursci., 13(7):1429-1434.

[20]Ni, Y.N., Wang, Y.R., Kokot, S., 2006. Simultaneous determination of three fluoroquinolones by linear sweep stripping voltammetry with the aid of chemometrics. Talanta, 69(1):216-225.

[21]Ordóñez, S.S., Fàbregas, E., 2007. New antibodies immobilization system into a graphite-polysulfone membrane for amperometric immunosensors. Biosens. Bioelectron., 22(6):965-972.

[22]Overholser, B.R., Kays, M.B., Sowinski, K.M., 2003. Determination of gatifloxacin in human serum and urine by high-performance liquid chromatography with ultraviolet detection. J. Chromatogr. B, 798(1):167-173.

[23]Perry, C.M., Barman Balfour, J.A., Lamb, H.M., 1999. Gatifloxacin. Drugs, 58(4):683-696.

[24]Piermarini, S., Micheli, L., Ammida, N.H.S., Palleschi, G., Moscone, D., 2007. Electrochemical immunosensor array using a 96-well screen-printed microplate for aflatoxin B-1 detection. Biosens. Bioelectron., 22(7):1434-1440.

[25]Pihel, K., Walker, Q.D., Wightman, R.M., 1996. Overoxidized polypyrrole-coated carbon fiber microelectrodes for dopamine measurements with fast-scan cyclic voltammetry. Anal. Chem., 68(13):2084-2089.

[26]Santoro, M.I.R.M., Kassab, N.M., Singh, A.K., Kedor-Hackmam, E.R.M., 2006. Quantitative determination of gatifloxacin, levofloxacin, lomefloxacin and pefloxacin fluoroquinolonic antibiotics in pharmaceutical preparations by high-performance liquid chromatography. J. Pharmaceut. Biomed. Anal., 40(1):179-184.

[27]Suhagia, B.N., Shah, S.A., Rathod, I.S., Patel, H.M., Shah, D.R., Marolia, B.P., 2006. Determination of gatifloxacin and ornidazole in tablet dosage forms by high-performance thin-layer chromatography. Anal. Sci., 22(5):743-745.

[28]Vishwanathan, K., Bartlett, M.G., Stewart, J.T., 2001. Determination of gatifloxacin in human plasma by liquid chromatography/electrospray tandem mass spectrometry. Rapid Commun. Mass Spectrom., 15(12):915-919.

[29]Weigel, L.M., Anderson, G.J., Tenover, F.C., 2002. DNA gyrase and topoisomerase IV mutations associated with fluoroquinolone resistance in Proteus mirabilis. Antimicrob. Agents Chemother., 46(8):2582-2587.

[30]Wightman, R.M., May, L.J., Michael, A.C., 1988. Detection of dopamine dynamics in the brain. Anal. Chem., 60(13):769-779.

[31]Yang, Z.Y., Kolosova, A.Y., Shim, W.B., Chung, D.H., 2006. Development of monoclonal antibodies against pirimiphos-methyl and their application to IC-ELISA. J. Agric. Food Chem., 54(13):4551-4556.

[32]Zhang, H.L., Lai, G.S., Han, D.Y., Yu, A.M., 2008. An amperometric hydrogen peroxide biosensor based on immobilization of horseradish peroxidase on an electrode modified with magnetic dextran microspheres. Anal. Bioanal. Chem., 390(3):971-977.

[33]Zhao, C., Liu, W., Ling, H., Lu, S., Zhang, Y., Liu, J., Xi, R., 2007. Preparation of anti-gatifloxacin antibody and development of an indirect competitive enzyme-linked immunosorbent assay for the detection of gatifloxacin residue in milk. J. Agric. Food Chem., 55(17):6879-6884.

[34]Zhou, M., Guan, C., Chen, G., Xie, X., Wu, S., 2005. Determination of theophylline concentration in serum by chemiluminescent immunoassay. J. Zhejiang Univ.-Sci. B, 6(12):1148-1152.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE