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CLC number: S859

On-line Access: 2011-11-04

Received: 2011-03-12

Revision Accepted: 2011-05-26

Crosschecked: 2011-10-12

Cited: 17

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.11 P.884-891

http://doi.org/10.1631/jzus.B1100076


Development of an indirect competitive ELISA for simultaneous detection of enrofloxacin and ciprofloxacin


Author(s):  Hai-tang Zhang, Jin-qing Jiang, Zi-liang Wang, Xin-yao Chang, Xing-you Liu, San-hu Wang, Kun Zhao, Jin-shan Chen

Affiliation(s):  College of Animal Science, Henan Institute of Science and Technology, Xinxiang 453003, China, Henan Higher Education Engineering Technology Research Center for Animal Diseases Control and Residues Supervision, Xinxiang 453003, China

Corresponding email(s):   jjq5678@126.com

Key Words:  Enrofloxacin, Ciprofloxacin, Indirect competitive ELISA, Animal tissues


Hai-tang Zhang, Jin-qing Jiang, Zi-liang Wang, Xin-yao Chang, Xing-you Liu, San-hu Wang, Kun Zhao, Jin-shan Chen. Development of an indirect competitive ELISA for simultaneous detection of enrofloxacin and ciprofloxacin[J]. Journal of Zhejiang University Science B, 2011, 12(11): 884-891.

@article{title="Development of an indirect competitive ELISA for simultaneous detection of enrofloxacin and ciprofloxacin",
author="Hai-tang Zhang, Jin-qing Jiang, Zi-liang Wang, Xin-yao Chang, Xing-you Liu, San-hu Wang, Kun Zhao, Jin-shan Chen",
journal="Journal of Zhejiang University Science B",
volume="12",
number="11",
pages="884-891",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100076"
}

%0 Journal Article
%T Development of an indirect competitive ELISA for simultaneous detection of enrofloxacin and ciprofloxacin
%A Hai-tang Zhang
%A Jin-qing Jiang
%A Zi-liang Wang
%A Xin-yao Chang
%A Xing-you Liu
%A San-hu Wang
%A Kun Zhao
%A Jin-shan Chen
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 11
%P 884-891
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100076

TY - JOUR
T1 - Development of an indirect competitive ELISA for simultaneous detection of enrofloxacin and ciprofloxacin
A1 - Hai-tang Zhang
A1 - Jin-qing Jiang
A1 - Zi-liang Wang
A1 - Xin-yao Chang
A1 - Xing-you Liu
A1 - San-hu Wang
A1 - Kun Zhao
A1 - Jin-shan Chen
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 11
SP - 884
EP - 891
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100076


Abstract: 
Modified 1-ethyl-3-(3-dimethylaminopropy) carbodiimide (EDC) method was employed to synthesize the artificial antigen of enrofloxacin (ENR), and New Zealand rabbits were used to produce anti-ENR polyclonal antibody (pAb). Based on the checkerboard titration, an indirect competitive enzyme-linked immunosorbent assay (ELISA) standard curve was established. This assay was sensitive and had a linear range from 0.6 to 148.0 μg/kg (R2=0.9567), with the half maximal inhibitory concentration (IC50) and limit of detection (LOD) values of 9.4 μg/kg and 0.2 μg/kg, respectively. Of all the competitive analogues, the produced pAb exhibited a high cross-reactivity to ciprofloxacin (CIP) (87%), the main metabolite of ENR in tissues. After optimization, the matrix effects can be ignored using a 10-fold dilution in beef and 20-fold dilution in pork. The overall recoveries and coefficients of variation (CVs) were in the ranges of 86%–109% and 6.8%–13.1%, respectively. It can be concluded that the established ELISA method is suitable for simultaneous detection of ENR and CIP in animal tissues.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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