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CLC number: R28

On-line Access: 2018-03-05

Received: 2017-02-25

Revision Accepted: 2017-06-12

Crosschecked: 2018-02-10

Cited: 0

Clicked: 3039

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Jian-mei Huang

https://orcid.org/0000-0002-5054-1011

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.3 P.227-244

http://doi.org/10.1631/jzus.B1700105


Metabolic profile of danshen in rats by HPLC-LTQ-Orbitrap mass spectrometry


Author(s):  Huan-huan Pang, Mei-fang Jiang, Qin-hui Wang, Xiao-ye Wang, Wei Gao, Zhi-hao Tian, Jian-mei Huang

Affiliation(s):  School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China

Corresponding email(s):   huangjm@bucm.edu.cn

Key Words:  Danshen, Chemical profile, Metabolic profile, HPLC-LTQ-Orbitrap


Huan-huan Pang, Mei-fang Jiang, Qin-hui Wang, Xiao-ye Wang, Wei Gao, Zhi-hao Tian, Jian-mei Huang. Metabolic profile of danshen in rats by HPLC-LTQ-Orbitrap mass spectrometry[J]. Journal of Zhejiang University Science B, 2018, 19(3): 227-244.

@article{title="Metabolic profile of danshen in rats by HPLC-LTQ-Orbitrap mass spectrometry",
author="Huan-huan Pang, Mei-fang Jiang, Qin-hui Wang, Xiao-ye Wang, Wei Gao, Zhi-hao Tian, Jian-mei Huang",
journal="Journal of Zhejiang University Science B",
volume="19",
number="3",
pages="227-244",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700105"
}

%0 Journal Article
%T Metabolic profile of danshen in rats by HPLC-LTQ-Orbitrap mass spectrometry
%A Huan-huan Pang
%A Mei-fang Jiang
%A Qin-hui Wang
%A Xiao-ye Wang
%A Wei Gao
%A Zhi-hao Tian
%A Jian-mei Huang
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 3
%P 227-244
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700105

TY - JOUR
T1 - Metabolic profile of danshen in rats by HPLC-LTQ-Orbitrap mass spectrometry
A1 - Huan-huan Pang
A1 - Mei-fang Jiang
A1 - Qin-hui Wang
A1 - Xiao-ye Wang
A1 - Wei Gao
A1 - Zhi-hao Tian
A1 - Jian-mei Huang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 3
SP - 227
EP - 244
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700105


Abstract: 
danshen, the dried root of Salvia miltiorrhiza Bunge (Lamiaceae), is one of the traditional Chinese medicines (TCMs) most commonly used for the treatment of cardiovascular and cerebrovascular diseases. However, little is known about the chemical and metabolic profiles of danshen in vitro or in vivo. In particular, more information is needed in relation to the 50% ethanol extracts usually used in danshen formulations such as Fufang Xueshuantong Capsules and Fufang danshen tablets. High-performance liquid chromatography coupled with a linear ion trap-Orbitrap mass spectrometer (HPLC-LTQ-Orbitrap) provides a sensitive and accurate method for analyzing the composition of samples. This method was used to determine the in vitro and in vivo chemical and metabolic profiles of danshen. Sixty-nine components of danshen extract and 118 components of danshen in rat plasma, urine, feces, and bile were unambiguously or tentatively identified. These results not only revealed the material composition of danshen, but also provided a comprehensive research approach for the identification of multi-constituents in TCMs.

HPLC-LTQ-Orbitrap方法分析丹参的代谢轮廓

目的:对丹参50%乙醇提取物的体内外物质基础进行系统全面的分析.
创新点:首次采用高效液相色谱-质谱联用(HPLC-LTQ-Orbitrap)方法对丹参50%乙醇提取物的体内外化学和代谢成分进行全面分析.
方法:建立HPLC-LTQ-Orbitrap方法,对丹参50%乙醇提取物的化学成分以及给药后大鼠血浆、尿液、粪便和胆汁生物样品中的化学和代谢成分进行分析.
结论:在正负离子模式下,在丹参提取物中鉴定出共69个化合物,包括丹酚酸类化合物23个,丹参酮类化合物33个,以及未知化合物13个;在大鼠
灌胃给予丹参提取物后的生物样本中鉴定出共118个化合物,包括血浆中35个,尿液中63个,粪便中62个,以及胆汁中18个.

关键词:丹参;化学轮廓;代谢轮廓;HPLC-LTQ-Orbitrap

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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