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On-line Access: 2018-03-05

Received: 2017-02-25

Revision Accepted: 2017-06-12

Crosschecked: 2018-02-10

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Citations:  Bibtex RefMan EndNote GB/T7714


Jian-mei Huang


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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.3 P.227-244


Metabolic profile of danshen in rats by HPLC-LTQ-Orbitrap mass spectrometry

Author(s):  Huan-huan Pang, Mei-fang Jiang, Qin-hui Wang, Xiao-ye Wang, Wei Gao, Zhi-hao Tian, Jian-mei Huang

Affiliation(s):  School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China

Corresponding email(s):   huangjm@bucm.edu.cn

Key Words:  Danshen, Chemical profile, Metabolic profile, HPLC-LTQ-Orbitrap

Huan-huan Pang, Mei-fang Jiang, Qin-hui Wang, Xiao-ye Wang, Wei Gao, Zhi-hao Tian, Jian-mei Huang. Metabolic profile of danshen in rats by HPLC-LTQ-Orbitrap mass spectrometry[J]. Journal of Zhejiang University Science B, 2018, 19(3): 227-244.

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author="Huan-huan Pang, Mei-fang Jiang, Qin-hui Wang, Xiao-ye Wang, Wei Gao, Zhi-hao Tian, Jian-mei Huang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Metabolic profile of danshen in rats by HPLC-LTQ-Orbitrap mass spectrometry
%A Huan-huan Pang
%A Mei-fang Jiang
%A Qin-hui Wang
%A Xiao-ye Wang
%A Wei Gao
%A Zhi-hao Tian
%A Jian-mei Huang
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 3
%P 227-244
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700105

T1 - Metabolic profile of danshen in rats by HPLC-LTQ-Orbitrap mass spectrometry
A1 - Huan-huan Pang
A1 - Mei-fang Jiang
A1 - Qin-hui Wang
A1 - Xiao-ye Wang
A1 - Wei Gao
A1 - Zhi-hao Tian
A1 - Jian-mei Huang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 3
SP - 227
EP - 244
%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1700105

danshen, the dried root of Salvia miltiorrhiza Bunge (Lamiaceae), is one of the traditional Chinese medicines (TCMs) most commonly used for the treatment of cardiovascular and cerebrovascular diseases. However, little is known about the chemical and metabolic profiles of danshen in vitro or in vivo. In particular, more information is needed in relation to the 50% ethanol extracts usually used in danshen formulations such as Fufang Xueshuantong Capsules and Fufang danshen tablets. High-performance liquid chromatography coupled with a linear ion trap-Orbitrap mass spectrometer (HPLC-LTQ-Orbitrap) provides a sensitive and accurate method for analyzing the composition of samples. This method was used to determine the in vitro and in vivo chemical and metabolic profiles of danshen. Sixty-nine components of danshen extract and 118 components of danshen in rat plasma, urine, feces, and bile were unambiguously or tentatively identified. These results not only revealed the material composition of danshen, but also provided a comprehensive research approach for the identification of multi-constituents in TCMs.




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