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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.2 P.87-92


Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C

Author(s):  Da-lei ZHANG, Kai-ming WANG, Cai-qiao ZHANG

Affiliation(s):  Key Laboratory of Animal Epidemic Etiology and Immunological Prevention of the Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   cqzhang@zju.edu.cn

Key Words:  Ginsenosides, Spermatogonia, Protein kinase C, Mouse

Da-lei ZHANG, Kai-ming WANG, Cai-qiao ZHANG. Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C[J]. Journal of Zhejiang University Science B, 2009, 10(2): 87-92.

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T1 - Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C
A1 - Da-lei ZHANG
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DOI - 10.1631/jzus.B0820133

The effect of ginsenosides on proliferation of type A spermatogonia was investigated in 7-day-old mice. spermatogonia were characterized by c-kit expression and cell proliferation was assessed by immunocytochemical demonstration of proliferating cell nuclear antigen (PCNA). After 72-h culture, Sertoli cells formed a confluent monolayer to which numerous spermatogonial colonies attached. spermatogonia were positive for c-kit staining and showed high proliferating activity by PCNA expression. ginsenosides (1.0~10 μg/ml) significantly stimulated proliferation of spermatogonia. Activation of protein kinase C (PKC) elicited proliferation of spermatogonia at 10−8 to 10−7 mol/L and the PKC inhibitor H7 inhibited this effect. Likewise, ginsenosides-stimulated spermatogonial proliferation was suppressed by combined treatment of H7. These results indicate that the proliferating effect of ginsenosides on mouse type A spermatogonia might be mediated by a mechanism involving the PKC signal transduction pathway.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Attele, A.S., Wu, J.A., Yuan, C.S., 1999. Ginseng pharmacology multiple constituents and multiple actions. Biochem. Pharmacol., 58(11):1685-1693.

[2] Chan, L., Chiu, P.Y., Lau, T.K., 2003. An in-vitro study of ginsenoside Rb1-induced teratogenicity using a whole rat embryo culture model. Hum. Reprod., 18(10):2166-2168.

[3] de Rooij, D.G., Grootegoedt, J.A., 1998. Spermatogonial stem cells. Curr. Opin. Cell Biol., 10(6):694-701.

[4] Dym, M., Jia, M.C., Dirami, G., Price, J.M., Rabin, S.J., Mocchetti, I., Ravindranath, N., 1995. Expression of c-kit receptor and its autophosphorylation in immature rat type A spermatogonia. Biol. Reprod., 52(1):8-19.

[5] Ge, C.T., Zhang, C.Q., Ye, J., Tang, X.Y., Wu, Y.Q., 2007. Ginsenosides promote proliferation of chicken primordial germ cells via PKC-involved activation of NF-κB. Cell Biol. Int., 31(10):1251-1256.

[6] Joo, S.S., Won, T.J., Kim, M.S., Lee, D.I., 2004. Hematopoietic effect of ginsenoside Rg3 in ICR mouse primary cultures and its application to a biological response modifier. Fitoterapia, 75(3-4):337-341.

[7] Kim, Y.S., Kim, D.S., Kim, S.I., 1998. Ginsenoside Rh2 and Rh3 induce differentiation of HL-60 cells into granulocytes: modulation of protein kinase C isoforms during differentiation by ginsenoside Rh2. Int. J. Biochem. Cell Biol., 30(3):327-338.

[8] Lin, T., Wang, D.L., Calkins, J.H., Guo, H., Chi, R., Housley, P.R., 1990. Regulation of insulin-like growth factor-I messenger ribonucleic acid expression in Leydig cells. Mol. Cell. Endocrinol., 73(2-3):147-152.

[9] Liu, H.Y., Zhang, C.Q., 2006. Ginsenosides promote proliferation of cultured ovarian germ cells involving protein kinase C-mediated system in embryonic chickens. Asian-Aust. J. Anim. Sci., 19(7):958-963.

[10] Liu, P., Yin, H.J., Xu, Y.J., Zhang, Z.F., Chen, K.J., Li, Y., 2006. Effects of ginsenoside Rg1 on postimplantation rat and mouse embryos cultured in vitro. Toxicol. Vitro, 20(2):234-238.

[11] Motohashi, H.H., Murata, K., Kada, H.H., 2000. Development of tetraploid and tetraploid/diploid mosaic mouse embryos induced by protein kinase C activators. J. Reprod. Dev., 46(3):193-200.

[12] Okuda, K., Uenoyama, Y., Lee, K.W., Sakumoto, R., Skarzynski, D.J., 1998. Progesterone stimulation by prostaglandin F2α involves the protein kinase C pathway in cultured bovine luteal cells. J. Reprod. Dev., 44(1): 79-84.

[13] Rajput, Z.I., Hu, S.H., Xiao, C.W., Arijo, A.G., 2007. Adjuvant effects of saponins on animal immune responses. J. Zhejiang Univ. Sci. B, 8(3):153-161.

[14] Tang, X.Y., Zhang, C.Q., 2007. Activation of protein kinases A and C promoted proliferation of chicken primordial germ cells. Anim. Reprod. Sci., 101(3-4):295-303.

[15] Wang, H.Z., Wang, H.K., Xiong, W.P., Chen, Y.M., Ma, Q.H., Ma, J., Ge, Y.H., Han, D.S., 2006. Evaluation on the phagocytosis of apoptotic spermatogenic cells by Sertoli cells in vitro through detecting lipid droplet formation by Oil Red O staining. Reproduction, 132(3):485-492.

[16] Yu, W.J., Lee, B.J., Nam, S.Y., Yang, D.C., Yun, Y.W., 2003. Modulating effects of Korean ginseng saponins on ovarian function in immature rats. Biol. Pharmacol. Bull., 26(11):1574-1580.

[17] Zhang, D.L., Zhang, C.Q., Liu, J.X., Hu, S.H., 2006. Ginsenosides promote meiotic maturation of mouse oocytes in cumulus oocyte complexes involving increased expression of nitric oxide synthase. Nutr. Res., 26(11):585-590.

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