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On-line Access: 2010-12-09

Received: 2010-03-05

Revision Accepted: 2010-05-10

Crosschecked: 2010-11-10

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.12 P.952-957

http://doi.org/10.1631/jzus.B1000073


Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation


Author(s):  Hong-yun Liu, Wei-dong Zeng, Ai-ling Cao, Cai-qiao Zhang

Affiliation(s):  Key Laboratory of Animal Epidemic Etiology and Immunological Prevention of the Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China, Xiaoshan Entry-Exit Inspection and Quarantine Bureau, Hangzhou 311208, China

Corresponding email(s):   cqzhang@zju.edu.cn

Key Words:  Follicle-stimulating hormone, Protein kinase A, Protein kinase C, Germ cell, Proliferation


Hong-yun Liu, Wei-dong Zeng, Ai-ling Cao, Cai-qiao Zhang. Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation[J]. Journal of Zhejiang University Science B, 2010, 11(12): 952-957.

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author="Hong-yun Liu, Wei-dong Zeng, Ai-ling Cao, Cai-qiao Zhang",
journal="Journal of Zhejiang University Science B",
volume="11",
number="12",
pages="952-957",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000073"
}

%0 Journal Article
%T Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation
%A Hong-yun Liu
%A Wei-dong Zeng
%A Ai-ling Cao
%A Cai-qiao Zhang
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 12
%P 952-957
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000073

TY - JOUR
T1 - Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation
A1 - Hong-yun Liu
A1 - Wei-dong Zeng
A1 - Ai-ling Cao
A1 - Cai-qiao Zhang
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 12
SP - 952
EP - 957
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000073


Abstract: 
The study was conducted to investigate the effects of follicle-stimulating hormone (FSH) on embryonic chicken ovarian germ cell proliferation and its possible involvements of protein kinases A (PKA) and C (PKC) pathways. Ovarian cells were treated with FSH alone or in the presence of forskolin (FRSK), PKA inhibitor (H89), PKC activator (PMA) or inhibitor (H7). The germ cell number was counted from micropictures. The immunocytochemistry of proliferating cell nuclear antigen (PCNA) was applied to identify the proliferating cells. The germ cell labeling index (LI) was determined for cell proliferation. The FSH treatment increased the germ cell number, and this stimulating effect was enhanced by FRSK or PMA, but inhibited by H89 or H7 in a dose-dependent manner. Moreover, the PCNA-LI showed parallel changes with germ cell numbers. This study suggests that FSH may stimulate proliferation of cultured chicken ovarian germ cells by activation of both the PKA and PKC signaling pathways.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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