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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.2 P.142-146


Transient expression of organophosphorus hydrolase to enhance the degrading activity of tomato fruit on coumaphos

Author(s):  Jie-hong ZHAO, De-gang ZHAO

Affiliation(s):  MOE Key Laboratory of Green Pesticide and Agricultural Bioengineering; more

Corresponding email(s):   dgzhao@gzu.edu.cn

Key Words:  Bioremediation, E8 promoter, Organophosphorus hydrolase (OPH), Transient expression

Jie-hong ZHAO, De-gang ZHAO. Transient expression of organophosphorus hydrolase to enhance the degrading activity of tomato fruit on coumaphos[J]. Journal of Zhejiang University Science B, 2009, 10(2): 142-146.

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%T Transient expression of organophosphorus hydrolase to enhance the degrading activity of tomato fruit on coumaphos
%A Jie-hong ZHAO
%A De-gang ZHAO
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T1 - Transient expression of organophosphorus hydrolase to enhance the degrading activity of tomato fruit on coumaphos
A1 - Jie-hong ZHAO
A1 - De-gang ZHAO
J0 - Journal of Zhejiang University Science B
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EP - 146
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Y1 - 2009
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B0820251

We constructed an expression cassette of the organophosphorus pesticide degrading (opd) gene under the control of the e8 promoter. Then opd was transformed into tomato fruit using an agroinfiltration transient expression system. β-Glucuronidase (GUS) staining, reverse transcription-polymerase chain reaction (RT-PCR), wavelength scanning, and fluorescent reaction were performed to examine the expression of the opd gene and the hydrolysis activity on coumaphos of organophosphorus hydrolase (OPH) in tomato fruit. The results show that the agroinfiltrated tomato fruit-expressed OPH had the maximum hydrolysis activity of about 11.59 U/mg total soluble protein. These results will allow us to focus on breeding transgenic plants that could not only enhance the degrading capability of fruit and but also hold no negative effects on pest control when spraying organophosphorus pesticides onto the seedlings in fields.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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