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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.7 P.522-527


Features of intervertebral disc degeneration in rat’s aging process

Author(s):  Yin-gang ZHANG, Zheng-ming SUN, Jiang-tao LIU, Shi-jie WANG, Feng-ling REN, Xiong GUO

Affiliation(s):  Department of Orthopaedics, the First Affiliated Hospital, Medical College of Xi’ more

Corresponding email(s):   zyingang@mail.xjtu.edu.cn

Key Words:  Intervertebral disc, Degeneration, Afferent vascular bud, Calcified layer, Aged

Yin-gang ZHANG, Zheng-ming SUN, Jiang-tao LIU, Shi-jie WANG, Feng-ling REN, Xiong GUO. Features of intervertebral disc degeneration in rat’s aging process[J]. Journal of Zhejiang University Science B, 2009, 10(7): 522-527.

@article{title="Features of intervertebral disc degeneration in rat’s aging process",
author="Yin-gang ZHANG, Zheng-ming SUN, Jiang-tao LIU, Shi-jie WANG, Feng-ling REN, Xiong GUO",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Features of intervertebral disc degeneration in rat’s aging process
%A Yin-gang ZHANG
%A Zheng-ming SUN
%A Jiang-tao LIU
%A Shi-jie WANG
%A Feng-ling REN
%A Xiong GUO
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 7
%P 522-527
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820295

T1 - Features of intervertebral disc degeneration in rat’s aging process
A1 - Yin-gang ZHANG
A1 - Zheng-ming SUN
A1 - Jiang-tao LIU
A1 - Shi-jie WANG
A1 - Feng-ling REN
A1 - Xiong GUO
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 7
SP - 522
EP - 527
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820295

Objective: The age-related change is important part of degenerative disc disease. However, no appropriate animal model or objective evaluation index is available. This study aimed to investigate the features of intervertebral disc degeneration in aging process of rats. Methods: 22-month-old Sprague-Dawley (SD) rats were used as spontaneously occurring intervertebral disc degeneration models and 6-month-old rats as young controls. Expression of collagen types II and X was measured by immunohistochemistry. degenerations of intervertebral discs were scored according to Miyamoto’s method. Numbers and areas of afferent vascular buds were measured. The thicknesses of non-calcified and calcified layers were measured and statistically analyzed. Results: There were less collagen type II expression and more collagen type X expression in the calcified layer of the cartilage endplates and nucleus pulposus in the rats of the aged group than in the young control. There were fewer and smaller afferent vascular buds in the rats of the aged group than in the young control group. The ratio of the non-calcified to the calcified layers in the rats of the aged group significantly decreased, compared with that of the young control group (P<0.01). Conclusion: Rats can spontaneously establish intervertebral disc age-related degeneration. The expression of collagen types II and X, numbers and areas of afferent vascular buds, the ratio of the non-calcified to the calcified layers, and water and glycosaminoglycan contents in the nucleus pulposus are sensitive indexes of intervertebral disc degeneration.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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