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On-line Access: 2023-07-15

Received: 2022-11-20

Revision Accepted: 2023-01-06

Crosschecked: 2023-07-17

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Jiuliang ZHANG

https://orcid.org/0000-0002-1745-846X

Qing ZHOU

https://orcid.org/0009-0000-0761-4572

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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.7 P.587-601

http://doi.org/10.1631/jzus.B2200587


Diacylated anthocyanins from purple sweet potato (Ipomoea batatas L.) attenuate hyperglycemia and hyperuricemia in mice induced by a high-fructose/high-fat diet


Author(s):  Luhong SHEN, Yang YANG, Jiuliang ZHANG, Lanjie FENG, Qing ZHOU

Affiliation(s):  College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; more

Corresponding email(s):   zjl_ljz@mail.hzau.edu.cn, qingz_zqing@aliyun.com

Key Words:  Diacylated anthocyanins from purple sweet potato (diacylated AF-PSPs), Hyperglycemia, Hyperuricemia, Metabolism syndrome, Regulation of renal function


Luhong SHEN, Yang YANG, Jiuliang ZHANG, Lanjie FENG, Qing ZHOU. Diacylated anthocyanins from purple sweet potato (Ipomoea batatas L.) attenuate hyperglycemia and hyperuricemia in mice induced by a high-fructose/high-fat diet[J]. Journal of Zhejiang University Science B, 2023, 24(7): 587-601.

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%T Diacylated anthocyanins from purple sweet potato (Ipomoea batatas L.) attenuate hyperglycemia and hyperuricemia in mice induced by a high-fructose/high-fat diet
%A Luhong SHEN
%A Yang YANG
%A Jiuliang ZHANG
%A Lanjie FENG
%A Qing ZHOU
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A1 - Luhong SHEN
A1 - Yang YANG
A1 - Jiuliang ZHANG
A1 - Lanjie FENG
A1 - Qing ZHOU
J0 - Journal of Zhejiang University Science B
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DOI - 10.1631/jzus.B2200587


Abstract: 
Studies have shown that targeting xanthine oxidase (XO) can be a feasible treatment for fructose-induced hyperuricemia and hyperglycemia. This study aimed to evaluate the dual regulatory effects and molecular mechanisms of diacylated anthocyanins from purple sweet potato (diacylated AF-PSPs) on hyperglycemia and hyperuricemia induced by a high-fructose/high-fat diet. The body weight, organ index, serum biochemical indexes, and liver antioxidant indexes of mice were measured, and the kidneys were observed in pathological sections. The relative expression levels of messenger RNAs (mRNAs) of fructose metabolism pathway enzymes in kidney were detected by fluorescent real-time quantitative polymerase chain (qPCR) reaction technique, and the expression of renal transporter protein and inflammatory factor pathway protein was determined by immunohistochemistry (IHC) technique. Results showed that diacylated AF-PSPs alleviated hyperuricemia in mice, and that this effect might be related to the regulation of liver XO activity, lipid accumulation, and relevant renal transporters. Diacylated AF-PSPs reduced body weight and relieved lipid metabolism disorder, liver lipid accumulation, and liver oxidative stress, thereby enhancing insulin utilization and sensitivity, lowering blood sugar, and reducing hyperglycemia in mice. Also, diacylated AF-PSPs restored mRNA levels related to renal fructose metabolism, and reduced kidney injury and inflammation. This study provided experimental evidence for the mechanisms of dual regulation of blood glucose and uric acid (UA) by diacylated AF-PSPs and their utilization as functional foods in the management of metabolic syndrome.

紫薯双酰基花色苷对高果糖/高脂肪饮食诱导的小鼠高血糖和高尿酸血症的调节作用

沈路虹1,杨扬1,张久亮1,冯兰杰1,周庆2
1华中农业大学食品科学技术学院,中国武汉市,430070
2华中科技大学同济医学院附属武汉市中心医院药学部,中国武汉市,430014
摘要:多数研究已表明针对黄嘌呤氧化酶(XO)可以成为治疗果糖诱导的高尿酸血症和高血糖症的可行方法,然而本研究旨在评估紫薯双酰基花色苷对高果糖/高脂肪饮食诱发的高血糖和高尿酸血症的双重调节作用及其分子机制。试验对小鼠体重、脏器指数、血清生化指标及肝脏抗氧化指标进行检测,并对小鼠肾脏进行病理切片观察。利用实时荧光定量聚合酶链反应检测小鼠肾脏中果糖代谢通路酶的mRNA相对表达量,同时,利用免疫组织化学技术测定小鼠肾脏转运蛋白和炎症因子通路蛋白表达量。研究结果显示:双酰基花色苷可以缓解小鼠的高尿酸血症,这种作用可能与其调节肝脏XO活性、脂质积累和相关的肾脏转运蛋白有关;紫薯双酰基花色苷能够减轻小鼠体重,缓解小鼠脂质代谢紊乱,降低肝脏脂质积累和肝脏氧化应激,从而提高其胰岛素利用率和敏感性,同时能够降低血糖,减少高血糖的发生;此外,双酰基花色苷恢复了与肾脏果糖代谢有关的mRNA水平,并减轻了肾脏损伤和炎症。这项研究为紫薯双酰基花色苷对血糖和尿酸的双重调节机制及其作为功能食品在代谢综合征治疗中的应用提供了实验依据。

关键词:紫薯双酰基花色苷;高血糖;高尿酸血症;代谢综合征;肾功能调节

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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