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On-line Access: 2010-09-08

Received: 2010-01-02

Revision Accepted: 2010-04-16

Crosschecked: 2010-08-09

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.9 P.702-707


Developing new SSR markers from ESTs of pea (Pisum sativum L.)

Author(s):  Ya-ming Gong, Sheng-chun Xu, Wei-hua Mao, Qi-zan Hu, Gu-wen Zhang, Ju Ding, Ya-dan Li

Affiliation(s):  Institute of Vegetables, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China, Center of Analysis and Measurement, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   gongym07@126.com

Key Words:  Pea, Expressed sequence tag (EST), Simple sequence repeat (SSR), Microsatellite

Ya-ming Gong, Sheng-chun Xu, Wei-hua Mao, Qi-zan Hu, Gu-wen Zhang, Ju Ding, Ya-dan Li. Developing new SSR markers from ESTs of pea (Pisum sativum L.)[J]. Journal of Zhejiang University Science B, 2010, 11(9): 702-707.

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author="Ya-ming Gong, Sheng-chun Xu, Wei-hua Mao, Qi-zan Hu, Gu-wen Zhang, Ju Ding, Ya-dan Li",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Developing new SSR markers from ESTs of pea (Pisum sativum L.)
%A Ya-ming Gong
%A Sheng-chun Xu
%A Wei-hua Mao
%A Qi-zan Hu
%A Gu-wen Zhang
%A Ju Ding
%A Ya-dan Li
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 9
%P 702-707
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000004

T1 - Developing new SSR markers from ESTs of pea (Pisum sativum L.)
A1 - Ya-ming Gong
A1 - Sheng-chun Xu
A1 - Wei-hua Mao
A1 - Qi-zan Hu
A1 - Gu-wen Zhang
A1 - Ju Ding
A1 - Ya-dan Li
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 9
SP - 702
EP - 707
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000004

The development of expressed sequence tags (ESTs) from pea has provided a useful source for mining novel simple sequence repeat (SSR) markers. In the present research, in order to find EST-derived SSR markers, 18 552 pea ESTs from the National Center for Biotechnology Information (NCBI) database were downloaded and assembled into 10 086 unigenes. A total of 586 microsatellites in 530 unigenes were identified, indicating that merely 5.25% of sequences contained SSRs. The most abundant SSRs within pea were tri-nucleotide repeat motifs, and among all the tri-nucleotide repeats, the motif GAA was the most abundant type. In total, 49 SSRs were used for primer design. EST-SSR loci were subsequently screened on 10 widely adapted varieties in China. Of these, nine loci showed polymorphic profiles that revealed two to three alleles per locus. The polymorphism information content value ranged from 0.18 to 0.58 with an average of 0.41. Furthermore, transferable analysis revealed that some of these loci showed transferability to faba bean. Because of their polymorphism and transferability, these nine novel EST-SSRs will be valuable tools for marker-assisted breeding and comparative mapping of pea in the future.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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