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CLC number: Q949.95

On-line Access: 2011-05-06

Received: 2010-05-16

Revision Accepted: 2010-11-15

Crosschecked: 2011-04-12

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.5 P.393-398

http://doi.org/10.1631/jzus.B1000179


Molecular authentication of geo-authentic Scrophularia ningpoensis


Author(s):  Chuan Chen, Li-na Duan, Xiao-long Zhou, Bing-long Chen, Cheng-xin Fu

Affiliation(s):  Key Laboratory of Conservation Biology for Endangered Wildlife of Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, China, Laboratory of Systematic and Evolutionary Botany and Biodiversity, Institute of Plant Sciences and Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou 310058, China, Pan’an Institute of Traditional Chinese Medicine, Pan’an 322300, China

Corresponding email(s):   cxfu@zju.edu.cn

Key Words:  Inter-simple sequence repeat (ISSR), Sequence characterized amplified region (SCAR), Scrophularia ningpoensis, Chinese Materia Medica, Traditional Chinese medicine


Chuan Chen, Li-na Duan, Xiao-long Zhou, Bing-long Chen, Cheng-xin Fu. Molecular authentication of geo-authentic Scrophularia ningpoensis[J]. Journal of Zhejiang University Science B, 2011, 12(5): 393-398.

@article{title="Molecular authentication of geo-authentic Scrophularia ningpoensis",
author="Chuan Chen, Li-na Duan, Xiao-long Zhou, Bing-long Chen, Cheng-xin Fu",
journal="Journal of Zhejiang University Science B",
volume="12",
number="5",
pages="393-398",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000179"
}

%0 Journal Article
%T Molecular authentication of geo-authentic Scrophularia ningpoensis
%A Chuan Chen
%A Li-na Duan
%A Xiao-long Zhou
%A Bing-long Chen
%A Cheng-xin Fu
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 5
%P 393-398
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000179

TY - JOUR
T1 - Molecular authentication of geo-authentic Scrophularia ningpoensis
A1 - Chuan Chen
A1 - Li-na Duan
A1 - Xiao-long Zhou
A1 - Bing-long Chen
A1 - Cheng-xin Fu
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 5
SP - 393
EP - 398
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000179


Abstract: 
Scrophularia ningpoensis has long been used in the chinese Materia Medica for inflammation. Like other herbal medicines, S. ningpoensis collected from different localities may considerably differ in their therapeutic efficacy, and the one grown in Zhejiang Province is recognized as geo-authentic. However, it is difficult to confirm the geographical authenticity by similar morphological characteristics. In the present study, inter-simple sequence repeat (ISSR) markers were conducted to detect S. ningpoensis from different origins. A 1 259-bp fragment amplified by primer UBC874 was found only in geo-authentic ones. By cloning and sequencing that specific band, sequence characterized amplified region (SCAR) markers were designed to distinguish geo-authentic S. ningpoensis from others. This is a rapid and easy method that can be used to identify the geographical authenticity of S. ningpoensis.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Camacho, F.J., Liston, A., 2001. Population structure and genetics diversity of Botrychium pumicola (Ophioglossaceae) based on ISSR. Am. J. Bot., 88(6):1065-1070.

[2]Chung, S.Y., Cheng, F.C., Lee, M.S., Lin, J.Y., Lin, M.C., Wang, M.F., 2006. Ginkgo biloba leaf extract (EGb761) combined with neuroprotective agents reduces the infarct volumes of gerbil ischemic brain. Am. J. Chin. Med., 34(5):803-817.

[3]de Santos Galíndez, J., Lanza, A.M.D., Matellano, L.F., 2002. Biologically active substances from the genus Scrophularia. Pharm. Biol., 40(1):45-59.

[4]Díaz, A.M., Abad, M.J., Fernández, L., Silván, A.M., de Santos, J., Bermejo, P., 2004. Phenylpropanoid glycosides from Scrophularia scorodonia: in vitro anti-inflammatory activity. Life Sci., 74(20):2515-2526.

[5]Doyle, J.J., 1991. DNA Protocols for Plants—CTAB Total DNA Isolation. In: Hewitt, G., Johnston, A. (Eds.), Molecular Techniques in Taxonomy. Springer Verlag, Berlin, Germany, p.283-294.

[6]Fernández, M.A., García, M.D., Sáenz, M.T., 1996. Antibacterial activity of the phenolic acids fraction of Scrophularia frutescens and Scrophularia sambucifolia. J. Ethnopharmacol., 53(1):11-14.

[7]Forbes, F.B., Hemsley, W.B., 1890. An enumeration of all the plants known from China proper, Formosa, Hainan, Corea, the Luchu archipelago, and the island of Hongkong together with their distribution and synonymy. J. Linn. Soc. Bot., 26(74):178-179.

[8]Giner, R.M., Villalba, M.L., Recio, M.C., Máñez, S., Cerdá-Nicolás, M., Ríos, J.L., 2000. Anti-inflammatory glycoterpenoids from Scrophularia auriculata. Eur. J. Pharmacol., 389(2-3):243-252.

[9]Gong, W., Fu, C.X., Luo, Y.P., Qiu, Y.X., 2006. Molecular identification of Sinopodophyllum hexandrum and Dysosma species using cpDNA sequences and PCR-RFLP markers. Planta Med., 72(7):650-652.

[10]Hu, P., Luo, G.A., Zhao, Z.Z., Jiang, Z.H., 2005. Multi-component HPLC fingerprinting of Radix Salviae Miltiorrhizae and its LC-MS-MS identification. Chem. Pharm. Bull. (Tokyo), 53(6):677-683.

[11]Hu, Y., Zhang, Q., Xin, H., Qin, L.P., Lu, B.R., Rahman, K., 2007. Association between chemical and genetic variation of Vitex rotundifolia populations from different locations in China: its implication for quality control of medicinal plants. Biomed. Chromatogr., 21(9):967-975.

[12]Hua, R., Sun, S.Q., Zhou, Q., Noda, I., Wang, B.Q., 2003. Discrimination of fritillary according to geographical origin with Fourier transform infrared spectroscopy and two-dimensional correlation IR spectroscopy. J. Pharmaceut. Biomed., 33(2):199-209.

[13]Kajimoto, T., Hidaka, M., Shoyama, K., Nohara, T., 1989. Iridoids from Scrophularia ninpoensis. Phytochemistry, 28(10):2701-2704.

[14]Liu, C.W., Bi, Z.M., Zhu, Y.F., Li, P., 2007. Simultaneous determination of four kinds of bioactive components in Radix Scrophulariae by HPLC. China Pharm. J., 42(21):1614-1616 (in Chinese).

[15]Liu, L., Hudgins, W.R., Shack, S., Yin, M.Q., Samid, D., 1995. Cinnamic acid: a natural product with potential use in cancer intervention. Int. J. Cancer, 62(3):345-350.

[16]Lu, G.H., Chan, K., Liang, Y.Z., Leung, K.S.Y., Chan, C.L., Jiang, Z.H., 2005. Development of high performance liquid chromatographic fingerprints for distinguishing of Chinese Angelica from related umbelliferae herbs. J. Chromatogr. A, 1073(1-2):383-392.

[17]Lum, R.M., Potter, E., Dang, T., Heber, D., Hardy, M., Hirsch, M.A., 2005. Identification of botanicals and potential contaminants through RFLP and sequencing. Planta Med., 71(9):841-846.

[18]Miyase, T., Mimatsu, A., 1999. Acylated iridoid andphenylethanoid glysocides from the aerial parts of Scrophularia nodosa. J. Nat. Prod., 62(8):1079-1084.

[19]Miyazawa, M., Okuno, Y., Nakamura, S., Kameoka, H., 1998. Suppression of SOS-inducing activity of chemical mutagens by cinnamic acid derivatives from Scrophulia ningpoensis in the Salmonella typhimurium TA1535/ pSK1002 umu test. J. Agric. Food Chem., 46(3):904-910.

[20]Moraes, R.M., Momm, H.G., Silva, B., Maddox, V., Easson, G.L., Lata, H., Ferreira, D., 2005. Geographic information system method for assessing chemo-diversity in Medicinal plants. Planta Med., 71(12):1157-1164.

[21]Passinho-Soares, H., Felix, D., Maria Auxiliadora Kaplan, M.A., Margis-Pinheiro, M., Margis, R., 2006. Authentication of medicinal plant botanical identity by amplified fragmented length polymorphism dominant DNA marker: inferences from the Plectranthus genus. Planta Med., 72(10):929-931.

[22]Reid, D.P., 1996. Chinese Herbal Medicine. Shambhala Publications Inc. Press, Boston, US, p.98.

[23]Sagar, S.M., Yance, D., Wong, R.K., 2006. Natural health products that inhibit angiogenesis: a potential source for investigational new agents to treat cancer—Part 1. Curr. Oncol., 13(1):14-26.

[24]Techen, N., Khan, I.A., Pan, Z.Q., Scheffler, B.E., 2006. The use of polymerase chain reaction (PCR) for the identification of Ephedra DNA in dietary supplements. Planta Med., 72(3):241-247.

[25]Wang, J.K., Wang, J.L., 2007. History and Genuine Character of Traditional Chinese Medicine. Science and Technology of China Medicine Press, Beijing, China, p.94 (in Chinese).

[26]Woo, Y.A., Kim, H.J., Cho, J., Chung, H., 1999. Discrimination of herbal medicines according to geographical origin with near infrared reflectance spectroscopy and pattern recognition techniques. J. Pharmaceut. Biomed. Anal., 21(2):407-413.

[27]Yang, M., Zhang, D.M., Liu, J.Q., Zheng, J.H., 2001. A molecular marker that is specific to medicinal rhubarb based on chloroplast trnL/trnF sequences. Planta Med., 67(8):784-786.

[28]Yang, S., Chen, C., Zhao, Y., Xi, W., Zhou, X., Chen, B., Fu, C., 2010. Association between chemical and genetic variation of wild and cultivated populations of Scrophularia ningpoensis Hemsl. Planta Med., in press.

[29]Ye, Q., Qiu, Y.X., Quo, Y.Q., Chen, J.X., Yang, S.Z., Zhao, M.S., Fu, C.X., 2006. Species-specific SCAR markers for authentication of Sinocalycanthus chinensis. J. Zhejiang Univ.-Sci. B., 7(11):868-872.

[30]Yen, K.Y., 1992. The Illustrated Chinese Materia Medica Crude and Prepared. SMC Publishing Inc. Press, Taiwan, China, p.64 (in Chinese).

[31]Yip, P.Y., Kwan, H.S., 2006. Molecular identification of Astragalus membranaceus at the species and locality levels. J. Ethnopharmacol., 106(2):222-229.

[32]Zhang, Y.B., Ngan, F.N., Wang, Z.T., Ng, T.B., But, P.P., Shaw, P.C., 1999. Random primed polymerase chain reaction differentiates Codonopsis pilosula from different localities. Planta Med., 65(2):157-160.

[33]Zhao, Y.P., Qiu, Y.X., Gong, W., Fu, C.X., Li, J.H., 2007. Authentication of Actinidia macrosperma using PCR-RFLP based on trnK sequences. Botanical Studies, 48(3):239-242.

[34]Zhao, Z.Z., Hu, Y., Liang, Z.T., Yuen, J.P., Jiang, Z.H., Leung, K.S., 2006. Authentication is fundamental for standardization of Chinese Medicines. Planta Med., 72(10):865-874.

[35]Zhu, Y.F., Bi, Z.M., Liu, C.W., Ren, M.T., Wu, F.H., Li, P., 2008. Endothelial cell extraction and HPLC-ESI/TOF MS analysis for predicting potential bioactive components of Radix Scrophulariae. J. China Pharm. Univ., 39:228-231 (in Chinese).

[36]Zietkiewicz, E., Rafalski, A., Labuda, D., 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20(2):176-183.

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