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CLC number: R394

On-line Access: 2011-10-08

Received: 2010-11-28

Revision Accepted: 2011-07-27

Crosschecked: 2011-09-07

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.10 P.796-804

http://doi.org/10.1631/jzus.B1000411


Normal epigenetic inheritance in mice conceived by in vitro fertilization and embryo transfer


Author(s):  Lei Li, Fang Le, Li-ya Wang, Xiang-rong Xu, Hang-ying Lou, Ying-ming Zheng, Jiang-zhong Sheng, He-feng Huang, Fan Jin

Affiliation(s):  Centre of Reproductive Medicine, Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China, Department of Gynecologic Oncology, Henan Cancer Hospital, Zhengzhou 450003, China, Department of Cell Biology, School of Medicine, Zhejiang University, Hangzhou 310006, China

Corresponding email(s):   jinfan@zju.edu.cn

Key Words:  Differentially methylated regions (DMRs), In vitro fertilization and embryo transfer (IVF-ET), Central nervous system (CNS), Neurobehavioral imprinting disorders, Transgenerational epigenetic inheritance


Lei Li, Fang Le, Li-ya Wang, Xiang-rong Xu, Hang-ying Lou, Ying-ming Zheng, Jiang-zhong Sheng, He-feng Huang, Fan Jin. Normal epigenetic inheritance in mice conceived by in vitro fertilization and embryo transfer[J]. Journal of Zhejiang University Science B, 2011, 12(10): 796-804.

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author="Lei Li, Fang Le, Li-ya Wang, Xiang-rong Xu, Hang-ying Lou, Ying-ming Zheng, Jiang-zhong Sheng, He-feng Huang, Fan Jin",
journal="Journal of Zhejiang University Science B",
volume="12",
number="10",
pages="796-804",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000411"
}

%0 Journal Article
%T Normal epigenetic inheritance in mice conceived by in vitro fertilization and embryo transfer
%A Lei Li
%A Fang Le
%A Li-ya Wang
%A Xiang-rong Xu
%A Hang-ying Lou
%A Ying-ming Zheng
%A Jiang-zhong Sheng
%A He-feng Huang
%A Fan Jin
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 10
%P 796-804
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000411

TY - JOUR
T1 - Normal epigenetic inheritance in mice conceived by in vitro fertilization and embryo transfer
A1 - Lei Li
A1 - Fang Le
A1 - Li-ya Wang
A1 - Xiang-rong Xu
A1 - Hang-ying Lou
A1 - Ying-ming Zheng
A1 - Jiang-zhong Sheng
A1 - He-feng Huang
A1 - Fan Jin
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 10
SP - 796
EP - 804
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000411


Abstract: 
An association between assisted reproductive technology (ART) and neurobehavioral imprinting disorders has been reported in many studies, and it seems that ART may interfere with imprint reprogramming. However, it has never been explored whether epigenetic errors or imprinting disease susceptibility induced by ART can be inherited transgenerationally. Hence, the aim of this study was to determine the effect of in vitro fertilization and embryo transfer (IVF-ET) on transgenerational inheritance in an inbred mouse model. Mice derived from IVF-ET were outcrossed to wild-type C57BL/6J to obtain their female and male line F2 and F3 generations. Their behavior, morphology, histology, and DNA methylation status at several important differentially methylated regions (DMRs) were analyzed by Morris water maze, hematoxylin and eosin (H&E) staining, and bisulfite genomic sequencing. No significant differences in spatial learning or phenotypic abnormality were found in adults derived from IVF (F1) and female and male line F2 and F3 generations. A borderline trend of hypomethylation was found in H19 DMR CpG island 3 in the female line-derived F3 generation (0.40±0.118, P=0.086). Methylation status in H19/Igf2 DMR island 1, Igf2 DMR, KvDMR, and Snrpn DMR displayed normal patterns. Methylation percentage did not differ significantly from that of adults conceived naturally, and the expression of the genes they regulated was not disturbed. Transgenerational integrity, such as behavior, morphology, histology, and DNA methylation status, was maintained in these generations, which indicates that exposure of female germ cells to hormonal stimulation and gamete manipulation might not affect the individuals and their descendents.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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