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On-line Access: 2012-04-06

Received: 2011-10-10

Revision Accepted: 2012-02-28

Crosschecked: 2012-03-09

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.4 P.318-326


Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles

Author(s):  Tong-liang Bu, Yu-dong Jia, Jin-xing Lin, Yu-ling Mi, Cai-qiao Zhang

Affiliation(s):  Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   yulingmi@zju.edu.cn, cqzhang@zju.edu.cn

Key Words:  Quercetin, 3-Methyl-4-nitrophenol, Oxidative damage, Apoptosis, Germ cell, Testis

Tong-liang Bu, Yu-dong Jia, Jin-xing Lin, Yu-ling Mi, Cai-qiao Zhang. Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles[J]. Journal of Zhejiang University Science B, 2012, 13(4): 318-326.

@article{title="Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles",
author="Tong-liang Bu, Yu-dong Jia, Jin-xing Lin, Yu-ling Mi, Cai-qiao Zhang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles
%A Tong-liang Bu
%A Yu-dong Jia
%A Jin-xing Lin
%A Yu-ling Mi
%A Cai-qiao Zhang
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 4
%P 318-326
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100318

T1 - Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles
A1 - Tong-liang Bu
A1 - Yu-dong Jia
A1 - Jin-xing Lin
A1 - Yu-ling Mi
A1 - Cai-qiao Zhang
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 4
SP - 318
EP - 326
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100318

As a component of diesel exhaust particles, 3-Methyl-4-nitrophenol (4-nitro-m-cresol, PNMC) is also a metabolite of the insecticide fenitrothion and imposes hazardous effects on human health. In the present study, the alleviative effect of a common antioxidant flavonoid quercetin on mouse germ cells intoxicated by PNMC was investigated. Results showed that a single intraperitoneal injection of PNMC at 100 mg/kg induced severe testicular damage after one week. PNMC-treated mice showed a significant loss of germ cells (approximate 40% loss of round germ cells). PNMC caused an increase of hydroxyl radical and hydrogen peroxide production and lipid peroxidation, as well as a decrease in glutathione level, superoxide dismutase and glutathione peroxidase activities. Furthermore, treatment of PNMC increased expression of the pro-apoptotic protein Bax and decreased expression of the anti-apoptotic protein Bcl-XL in germ cells. In addition, testicular caspase-3 activity was significantly up-regulated and germ cell apoptosis was significantly increased in the PNMC-treated mice. In contrast, combined administration of quercetin at 75 mg/kg significantly attenuated PNMC-induced testicular toxicity. These results indicate that the antioxidant quercetin displays a remarkable protective effect on PNMC-induced oxidative damage in mouse testes and may represent an efficient supplement to attenuate reproductive toxicity by environmental toxicants to ensure healthy sperm production.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Adams, J.M., Cory, S., 1998. The Bcl-2 protein family: arbiters of cell survival. Science, 281(5381):1322-1326.

[2]Baulig, A., Garlatti, M., Bonvallot, V., Marchand, A., Barouki, R., Marano, F., Baeza-Squiban, A., 2003. Involvement of reactive oxygen species in the metabolic pathways triggered by diesel exhaust particles in human airway epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol., 285(3):L671-L679.

[3]Bu, T., Mi, Y., Zeng, W., Zhang, C., 2011. Protective effect of quercetin on cadmium-induced oxidative toxicity on germ cells in male mice. Anat. Rec., 294(3):520-526.

[4]Cao, G., Sofic, E., Prior, R.L., 1997. Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. Free Radic. Biol. Med., 22(5):749-760.

[5]Chao, C.L., Hou, Y.C., Chao, P.D.L., Weng, C.S., Ho, F.M., 2009. The antioxidant effects of quercetin metabolites on the prevention of high glucose-induced apoptosis of human umbilical vein endothelial cells. Brit. J. Nutr., 101(8):1165-1170.

[6]Furuta, C., Suzuki, A.K., Taneda, S., Kamata, K., Hayashi, H., Mori, Y., Li, C., Watanabe, G., Taya, K., 2004. Estrogenic activities of nitrophenols in diesel exhaust particles. Biol. Reprod., 70(5):1527-1533.

[7]Furuta, C., Li, C., Taneda, S., Suzuki, A.K., Kamata, K., Watanabe, G., Taya, K., 2005. Immunohistological study for estrogenic activities of nitrophenols in diesel exhaust particles. Endocrine, 27(1):33-36.

[8]Gustafsson, A.B., Gottlieb, R.A., 2007. Bcl-2 family members and apoptosis, taken to heart. Am. J. Physiol. Cell Physiol., 292(1):C45-C51.

[9]Han, J.Y., Takeshita, K., Utsumi, H., 2001. Noninvasive detection of hydroxyl radical generation in lung by diesel exhaust particles. Free Radic. Biol. Med., 30(5):516-525.

[10]Hengartner, M.O., 2000. The biochemistry of apoptosis. Nature, 407(6805):770-776.

[11]Hiura, T.S., Kaszubowski, M.P., Li, N., Nel, A.E., 1999. Chemicals in diesel exhaust particles generate reactive oxygen radicals and induce apoptosis in macrophages. J. Immunol., 163(10):5582-5591.

[12]Huang, C.H., Lin, L.Y., Tsai, M.S., Hsu, C.Y., Chen, H.W., Wang, T.D., Chang, W.T., Cheng, T.J., Chen, W.J., 2010. Acute cardiac dysfunction after short-term diesel exhaust particles exposure. Toxicol. Lett., 192(3):349-355.

[13]Izawa, H., Kohara, M., Aizawa, K., Suganuma, H., Inakuma, T., Watanabe, G., Taya, K., Sagai, M., 2008. Alleviative effects of quercetin and onion on male reproductive toxicity induced by diesel exhaust particles. Biosci. Biotechnol. Biochem., 72(5):1235-1241.

[14]Januario, D.A., Perin, P.M., Maluf, M., Lichtenfels, A.J., Nascimento Saldiva, P.H., 2010. Biological effects and dose-response assessment of diesel exhaust particles on in vitro early embryo development in mice. Toxicol. Sci., 117(1):200-208.

[15]Jia, Y., Lin, J., Mi, Y., Zhang, C., 2011. Quercetin attenuates cadmium-induced oxidative damage and apoptosis in granulosa cells from chicken ovarian follicles. Reprod. Toxicol., 31(4):477-485.

[16]Kim, B.M., Choi, Y.J., Han, Y., Yun, Y.S., Hong, S.H., 2009. N,N-dimethyl phytosphingosine induces caspase-8-dependent cytochrome c release and apoptosis through ROS generation in human leukemia cells. Toxicol. Appl. Pharm., 239(1):87-97.

[17]Landvik, N.E., Gorria, M., Arlt, V.M., Asare, N., Solhaug, A., Lagadic-Gossmann, D., Holme, J.A., 2007. Effects of nitrated-polycyclic aromatic hydrocarbons and diesel exhaust particle extracts on cell signalling related to apoptosis: possible implications for their mutagenic and carcinogenic effects. Toxicology, 231(2-3):159-174.

[18]Li, C., Taneda, S., Suzuki, A.K., Furuta, C., Watanabe, G., Taya, K., 2006. Anti-androgenic activity of 3-methyl-4-nitrophenol in diesel exhaust particles. Eur. J. Pharmacol., 543(1-3):194-199.

[19]Mi, Y., Zhang, C., Li, C., Taneda, S., Watanabe, G., Suzuki, A.K., Taya, K., 2009. Quercetin protects embryonic chicken spermatogonial cells from oxidative damage intoxicated with 3-methyl-4-nitrophenol in primary culture. Toxicol. Lett., 190(1):61-65.

[20]Miyabara, Y., Ichinose, T., Takano, H., Lim, H.B., Sagai, M., 1998. Effects of diesel exhaust on allergic airway inflammation in mice. J. Allergy Clin. Immunol., 102:805-812.

[21]Mori, Y., Kamata, K., Toda, N., Hayashi, H., Seki, K., Taneda, S., Yoshino, S., Sakushima, A., Sakata, M., Suzuki, A.K., 2003. Isolation of nitrophenols from diesel exhaust particles (DEP) as vasodilatation compounds. Biol. Pharm. Bull., 26(3):394-395.

[22]Noya, Y., Mikami, Y., Taneda, S., Mori, Y., Suzuki, A.K., Ohkura, K., Yamaki, K., Yoshino, S., Seki, K., 2008. Improvement of an efficient separation method for chemicals in diesel exhaust particles: analysis for nitrophenols. Environ. Sci. Pollut. Res. Int., 15(4):318-321.

[23]Park, S., Nam, H., Chung, N., Park, J.D., Lim, Y., 2006. The role of iron in reactive oxygen species generation from diesel exhaust particles. Toxicol. in Vitro, 20(6):851-857.

[24]Rice-Evans, C.A., Miller, N.J., Paganga, G., 1996. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic. Biol. Med., 20(7):933-956.

[25]Rodrigo, R., Bosco, C., 2006. Oxidative stress and protective effects of polyphenols: comparative studies in human and reodent kidney. A review. Comp. Biochem. Phys. C, 142(3-4):317-327.

[26]Romão, P.R., Tovar, J., Fonseca, S.G., Moraes, R.H., Cruz, A.K., Hothersall, J.S., Noronha-Dutra, A.A., Ferreira, S.H., Cunha, F.Q., 2006. Glutathione and the redox control system trypanothione/trypanothione reductase are involved in the protection of Leishmania spp. against nitrosothiol-induced cytotoxicity. Braz. J. Med. Biol. Res., 39(3):355-363.

[27]Ross, J.A., Kasum, C.M., 2002. Dietary flavonoids: bioavailability, metabolic effects, and safety. Annu. Rev. Nutr., 22:19-34.

[28]Rotruck, J.T., Pope, A.L., Ganther, H.E., Swanson, A.B., Hafeman, D.G., Hoekstra, W.G., 1973. Selenium-biochemical role as a component of glutathione peroxidase. Science, 179(4073):588-590.

[29]Watanabe, N., Oonuki, Y., 1999. Inhalation of diesel engine exhaust affects spermatogenesis in growing male rats. Environ. Health Perspect, 107(7):539-544.

[30]Wright, J.A., Hermonat, M.W., Hards, R.G., 1982. A degradation product of fenitrothion, 3-methyl-4-nitrophenol, is an inhibitor of mammalian ribonucleotide reductase. Bull. Environ. Contam. Toxicol., 28(4):480-483.

[31]Yoshida, S., Sagai, M., Oshio, S., Umeda, T., Ihara, T., Sugamata, M., Sugawara, I., Takeda, K., 1999. Exposure to diesel exhaust affects the male reproductive system of mice. Int. J. Androl., 22(5):307-315.

[32]Zhang, D.L., Mi, Y.L., Wang, K.M., Zeng, W.D., Zhang, C.Q., 2008. Attenuating effect of daidzein on polychlorinated biphenyls-induced oxidative toxicity in mouse testicular cells. J. Zhejiang Univ. Sci. B, 9(7):567-571.

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