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CLC number: R684.3

On-line Access: 2013-05-04

Received: 2012-11-11

Revision Accepted: 2013-01-15

Crosschecked: 2013-04-02

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.5 P.426-437


Effects of sodium ferulate on preventing steroid-induced femoral head osteonecrosis in rabbits

Author(s):  Lei Tian, Xiao-qian Dang, Chun-sheng Wang, Pei Yang, Chen Zhang, Kun-zheng Wang

Affiliation(s):  Department of Orthopedics, the Second Affiliated Hospital, College of Medicine, Xi’an Jiaotong University, Xi’an 710004, China

Corresponding email(s):   wkzh1955@gmail.com

Key Words:  Femoral head necrosis, Glucocorticoid, Sodium ferulate, Apoptosis, Rabbit

Lei Tian, Xiao-qian Dang, Chun-sheng Wang, Pei Yang, Chen Zhang, Kun-zheng Wang. Effects of sodium ferulate on preventing steroid-induced femoral head osteonecrosis in rabbits[J]. Journal of Zhejiang University Science B, 2013, 14(5): 426-437.

@article{title="Effects of sodium ferulate on preventing steroid-induced femoral head osteonecrosis in rabbits",
author="Lei Tian, Xiao-qian Dang, Chun-sheng Wang, Pei Yang, Chen Zhang, Kun-zheng Wang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of sodium ferulate on preventing steroid-induced femoral head osteonecrosis in rabbits
%A Lei Tian
%A Xiao-qian Dang
%A Chun-sheng Wang
%A Pei Yang
%A Chen Zhang
%A Kun-zheng Wang
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 5
%P 426-437
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200311

T1 - Effects of sodium ferulate on preventing steroid-induced femoral head osteonecrosis in rabbits
A1 - Lei Tian
A1 - Xiao-qian Dang
A1 - Chun-sheng Wang
A1 - Pei Yang
A1 - Chen Zhang
A1 - Kun-zheng Wang
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 5
SP - 426
EP - 437
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200311

The aim of this study is to investigate the effects and possible mechanisms of sodium ferulate (SF) on anti-apoptosis in steroid-induced femoral head osteonecrosis in rabbits. Japanese white rabbits were randomly divided into three groups (control group, treatment group, and model group), each with 24 rabbits. The model and treatment groups were first injected with an intravenous dose of horse serum, 10 ml/kg, three weeks later with an intravenous dose of 7.5 ml/kg, and two weeks later with an intramuscular dose of methylprednisolone, 45 mg/kg, three times in order to establish rabbit models of osteonecrosis. Concurrently, the treatment group was injected with intravenous doses of SF 20 mg/kg for two weeks, once per day. Three time points, Weeks 2, 4, and 8, were selected after modeling was completed. Osteonecrosis was verified by histopathology with haematoxylin-eosin (HE) staining. The apoptosis rate of osteonecrosis was observed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The apoptosis expressions of caspase-3 and Bcl-2 were analyzed by immunohistochemistry and Western blot. The rabbit models of osteonecrosis were successfully established and observed by HE staining. SF was effective in intervening in apoptosis and decreasing the apoptosis rate in femoral head necrosis by the immunohistochemistry and TUNEL assay (P<0.01). Western blot analysis indicated that there were statistical significances in the protein levels of caspase-3 and Bcl-2 (P<0.01). SF has a protective effect by reducing the incidence of early steroid-induced femoral head necrosis in rabbits, effectively intervening in apoptosis through decreasing caspase-3 expression and up-regulating Bcl-2 expression.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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