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CLC number: R574

On-line Access: 2016-03-07

Received: 2015-10-11

Revision Accepted: 2015-12-09

Crosschecked: 2016-02-15

Cited: 1

Clicked: 4391

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Xi-shuang Liu

http://orcid.org/0000-0002-5177-3535

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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.3 P.209-217

http://doi.org/10.1631/jzus.B1500248


Hydrogen sulfide from a NaHS source attenuates dextran sulfate sodium (DSS)-induced inflammation via inhibiting nuclear factor-κB


Author(s):  Xi Chen, Xi-shuang Liu

Affiliation(s):  Medical College, Qingdao University, Qingdao 266021, China; more

Corresponding email(s):   liunich2015@126.com

Key Words:  Hydrogen sulfide (H2S), Inflammation, Nuclear factor-κ, B (NF-κ, B), Dextran sulfate sodium (DSS)


Xi Chen, Xi-shuang Liu. Hydrogen sulfide from a NaHS source attenuates dextran sulfate sodium (DSS)-induced inflammation via inhibiting nuclear factor-κB[J]. Journal of Zhejiang University Science B, 2016, 17(3): 209-217.

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doi="10.1631/jzus.B1500248"
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DOI - 10.1631/jzus.B1500248


Abstract: 
This study investigated the alleviating effects of hydrogen sulfide (H2S), derived from sodium hydrosulfide (NaHS), on inflammation induced by dextran sulfate sodium (DSS) in both in vivo and in vitro models. We found that NaHS injection markedly decreased rectal bleeding, diarrhea, and histological injury in DSS-challenged mice. NaHS (20 μmol/L) reversed DSS-induced inhibition in cell viability in Caco-2 cells and alleviated pro-inflammation cytokine expression in vivo and in vitro, indicating an anti-inflammatory function for H2S. It was also found that H2S may regulate cytokine expression by inhibiting the nuclear factor-κ;b (NF-κ;b) signaling pathway. In conclusion, our results demonstrated that H2S alleviated DSS-induced inflammation in vivo and in vitro and that the signal mechanism might be associated with the NF-κB signaling pathway.

硫化氢通过抑制NF-κB信号通路对葡聚糖硫酸钠(DSS)诱导的炎症反应起到缓解效果

目的:硫化氢(H2S)具有抗氧化和抗炎反应的作用,但是在DSS诱导的结肠炎模型中的研究鲜有报道。因此,本文采用小鼠和人结肠上皮细胞系Caco-2为实验模型,研究了H2S在DSS诱导的炎症模型中的缓解效果。
创新点:(1)本研究采用体内和体外模型分别对H2S对DSS诱导的炎症缓解效果进行了验证,结果发现H2S具有抗炎作用;(2)本研究发现H2S能够抑制核转录因子κB(NF-κB)信号通路,从而对炎症起到缓解作用。
方法:采用DSS建立小鼠结肠炎模型,腹腔注射H2S供体硫化氢钠(NaHS);采用DSS诱导Caco-2炎症,然后处理H2S供体NaHS。收集小鼠结肠组织和细胞,进行反转录聚合酶链反应(RT-PCR)和蛋白质免疫印迹法(Western blot)分析炎症基因和NF-κB表达情况。
结论:H2S对DSS诱导的体内和体外炎症反应具有一定的缓解作用,其机制可能是通过影响了NF-κB信号通路。

关键词:硫化氢(H2S);炎症反应;核转录因子κB(NF-κB);葡聚糖硫酸钠(DSS)

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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