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CLC number: R931.71

On-line Access: 2013-06-03

Received: 2013-02-25

Revision Accepted: 2013-05-10

Crosschecked: 2013-08-13

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.9 P.785-792

http://doi.org/10.1631/jzus.B1300056


Promoting role of an endophyte on the growth and contents of kinsenosides and flavonoids of Anoectochilus formosanus Hayata, a rare and threatened medicinal Orchidaceae plant


Author(s):  Fu-sheng Zhang, Ya-li Lv, Yue Zhao, Shun-xing Guo

Affiliation(s):  Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China; more

Corresponding email(s):   sxguo1986@163.com

Key Words:  Anoectochilus formosanus, Endophyte, Secondary metabolite, Kinsenoside, Flavonoid


Fu-sheng Zhang, Ya-li Lv, Yue Zhao, Shun-xing Guo. Promoting role of an endophyte on the growth and contents of kinsenosides and flavonoids of Anoectochilus formosanus Hayata, a rare and threatened medicinal Orchidaceae plant[J]. Journal of Zhejiang University Science B, 2013, 14(9): 785-792.

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author="Fu-sheng Zhang, Ya-li Lv, Yue Zhao, Shun-xing Guo",
journal="Journal of Zhejiang University Science B",
volume="14",
number="9",
pages="785-792",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300056"
}

%0 Journal Article
%T Promoting role of an endophyte on the growth and contents of kinsenosides and flavonoids of Anoectochilus formosanus Hayata, a rare and threatened medicinal Orchidaceae plant
%A Fu-sheng Zhang
%A Ya-li Lv
%A Yue Zhao
%A Shun-xing Guo
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 9
%P 785-792
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300056

TY - JOUR
T1 - Promoting role of an endophyte on the growth and contents of kinsenosides and flavonoids of Anoectochilus formosanus Hayata, a rare and threatened medicinal Orchidaceae plant
A1 - Fu-sheng Zhang
A1 - Ya-li Lv
A1 - Yue Zhao
A1 - Shun-xing Guo
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 9
SP - 785
EP - 792
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300056


Abstract: 
Anoectochilus formosanus, commonly known as “Jewel Orchid”, is a Chinese folk medicine used to treat hypertension, diabetes, and heart disease. The existence of A. formosanus is currently threatened by habitat loss, human and animal consumption, etc. The highly potent medicinal activity of A. formosanus is due to its secondary metabolites, especially kinsenosides and flavonoids. This orchid also has a unique mycorrhizal relationship. Most adult orchids rely on endophytes for mineral nutrition and have complex interactions with them, which are related to plant growth, yield and changes in secondary metabolites. This study investigated the promoting role of F-23 fungus (genus Mycena) on the biomass and contents of kinsenosides and flavonoids of A. formosanus in pot culture. The following were observed after 10 weeks of symbiotic cultivation: increased shoot height, shoot dry weight, and leaf numbers by 16.6%, 31.3%, and 22.5%, respectively; increased contents of kinsenosides, isorhamnetin-3-O-β-D-rutinoside, and isorhamnetin-3-O-β-D-glucopyranoside by 85.5%, 226.1%, and 196.0%, respectively; some hyphae in epidermal cells dyed red and/or reddish brown by safranine; and, significantly reduced number of starch grains in cortical cells. Moreover, F-23 fungus significantly improved the kinsenoside and flavonoid contents of A. formosanus. These findings supported the reports that endophytes can alter the production of secondary metabolites in their plant hosts, although further physiological, genetic and ecological analyses are warranted.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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