Full Text:   <2444>

Summary:  <1769>

CLC number: R944.9

On-line Access: 2014-10-08

Received: 2014-03-05

Revision Accepted: 2014-07-09

Crosschecked: 2014-09-24

Cited: 1

Clicked: 7955

Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.10 P.888-899


Needle-free injection of insulin powder: delivery efficiency and skin irritation assessment*

Author(s):  Chun-yu Li1,2, Zhe-wei Wang2,5, Can Tu2,6, Jia-bo Wang2, Bing-qian Jiang2, Qi Li2,3, Ling-na Zeng2, Zhi-jie Ma3, Ping Zhang4, Yan-ling Zhao2, Ya-ming Zhang2, Dan Yan2, Rui Tan5, Xiao-he Xiao4

Affiliation(s):  1. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610000, China; more

Corresponding email(s):   pharm_sci@126.com

Key Words:  Powder needleless injection, Insulin, Transdermal drug delivery, Skin irritation

Chun-yu Li, Zhe-wei Wang, Can Tu, Jia-bo Wang, Bing-qian Jiang, Qi Li, Ling-na Zeng, Zhi-jie Ma, Ping Zhang, Yan-ling Zhao, Ya-ming Zhang, Dan Yan, Rui Tan, Xiao-he Xiao. Needle-free injection of insulin powder: delivery efficiency and skin irritation assessment[J]. Journal of Zhejiang University Science B, 2014, 15(10): 888-899.

@article{title="Needle-free injection of insulin powder: delivery efficiency and skin irritation assessment",
author="Chun-yu Li, Zhe-wei Wang, Can Tu, Jia-bo Wang, Bing-qian Jiang, Qi Li, Ling-na Zeng, Zhi-jie Ma, Ping Zhang, Yan-ling Zhao, Ya-ming Zhang, Dan Yan, Rui Tan, Xiao-he Xiao",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Needle-free injection of insulin powder: delivery efficiency and skin irritation assessment
%A Chun-yu Li
%A Zhe-wei Wang
%A Can Tu
%A Jia-bo Wang
%A Bing-qian Jiang
%A Qi Li
%A Ling-na Zeng
%A Zhi-jie Ma
%A Ping Zhang
%A Yan-ling Zhao
%A Ya-ming Zhang
%A Dan Yan
%A Rui Tan
%A Xiao-he Xiao
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 10
%P 888-899
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400065

T1 - Needle-free injection of insulin powder: delivery efficiency and skin irritation assessment
A1 - Chun-yu Li
A1 - Zhe-wei Wang
A1 - Can Tu
A1 - Jia-bo Wang
A1 - Bing-qian Jiang
A1 - Qi Li
A1 - Ling-na Zeng
A1 - Zhi-jie Ma
A1 - Ping Zhang
A1 - Yan-ling Zhao
A1 - Ya-ming Zhang
A1 - Dan Yan
A1 - Rui Tan
A1 - Xiao-he Xiao
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 10
SP - 888
EP - 899
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400065

insulin is widely used in treating diabetes, but still needs to be administered by needle injection. This study investigated a new needle-free approach for insulin delivery. A portable powder needleless injection (PNI) device with an automatic mechanical unit was designed. Its efficiency in delivering insulin was evaluated in alloxan-induced diabetic rabbits. The skin irritation caused by the device was investigated and the results were analyzed in relation to aerodynamic parameters. Inorganic salt-carried insulin powders had hypoglycemic effects, while raw insulin powders were not effective when delivered by PNI, indicating that salt carriers play an important role in the delivery of insulin via PNI. The relative delivery efficiency of phosphate-carried insulin powder using the PNI device was 72.25%. A safety assessment test showed that three key factors (gas pressure, cylinder volume, and nozzle distance) were related to the amount of skin irritation caused by the PNI device. Optimized injection conditions caused minimal skin lesions and are safe to use in practice. The results suggest that PNI has promising prospects as a novel technology for delivering insulin and other biological drugs.



Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Agnihotri, S.A., Nadagouda, N., Mallikarjuna, T.M., 2004. Recent advances on chitosan-based micro- and nanoparticles in drug delivery. J Control Release, 100(1):5-28. 

[2] Agu, R.U., Ugwoke, M.I., Armand, M., 2001. The lung as a route for systemic delivery of therapeutic proteins and peptide. Respir Res, 2(4):198-209. 

[3] Arora, A., Prausnitz, M.R., Mitragotri, S., 2008. Micro-scale devices for transdermal drug delivery. Int J Pharm, 364(2):227-236. 

[4] Burkoth, T.L., Bellhouse, B.J., Hewson, G., 1999. Transdermal and transmucosal powdered drug delivery. Crit Rev Ther Drug Carrier Syst, 16(4):331-384. 

[5] Chen, D., Erickson, C.A., Endres, R.L., 2001. Adjuvantation of epidermal powder immunization. Vaccine, 19(20-22):2908-2917. 

[6] Clark, A., Kuo, M.C., Newman, S., 2008. A comparison of the pulmonary bioavailability of powder and liquid aerosol formulations of salmon calcitonin. Pharm Res, 25(7):1583-1590. 

[7] Clarke, C., Carswell, B., 2007.  Principles of Astrophysical Fluid Dynamics. Cambridge University Press,London, UK :226-227. 

[8] Cui, F.D., Tao, A.J., Cun, D.M., 2007. Preparation of insulin loaded PLGA-Hp55 nanoparticles for oral delivery. J Pharm Sci, 96(2):421-427. 

[9] Danaei, G., Finucane, M.M., Lu, Y., 2011. National, regional, and global trends in fasting plasma glucose and diabetes prevalence since 1980: systematic analysis of health examination surveys and epidemiological studies with 370 country-years and 2.7 million participants. Lancet, 378(9785):31-40. 

[10] Dean, H.J., Chen, D., 2004. Epidermal powder immunization against influenza. Vaccine, 23(5):681-686. 

[11] D'Souza, R., Mutalik, S., Venkatesh, M., 2005. Nasal insulin gel as an alternate to parenteral insulin: formulation, preclinical, and clinical studies. AAPS PharmSciTech, 6(2):184-189. 

[12] Hussain, A., Arnold, J.J., Khan, M.A., 2004. Absorption enhancers in pulmonary protein delivery. J Control Release, 94(1):15-24. 

[13] Jaracz, S., Chen, J., Kuznetsova, L.V., 2005. Recent advances in tumor-targeting anticancer drug conjugates. Bioorg Med Chem, 13(17):5043-5054. 

[14] Jin, Y., Uchida, M., Wang, C.F., 2001. Transdermal microparticle delivery by a supersonic-Helios™ gun system. Acta Pharm Sin, (in Chinese),36(2):140-144. 

[15] Kendall, M., Mitchell, T., Wrighton-Smith, P., 2004. Intradermal ballistic delivery of micro-particles into excised human skin for pharmaceutical applications. J Biomech, 37(11):1733-1741. 

[16] Lesinski, G.B., Smithson, S.L., Srivastav, N., 2001. A DNA vaccine encoding a peptide mimic of Streptococcus pneumoniae serotype 4 capsular polysaccharide induces specific anti-carbohydrate antibodies in Balb/c mice. Vaccine, 19(13-14):1717-1726. 

[17] Li, H., Nookala, S., Re, F., 2007. Aluminum hydroxide adjuvants activate caspase-1 and induce IL-1β and IL-18 release. J Immunol, 178(8):5271-5276. 

[18] Matheis, W., Zott, A., Schwanig, M., 2001. The role of the adsorption process for production and control combined adsorbed vaccines. Vaccine, 20(1-2):67-73. 

[19] Mitchell, T.J., Kendall, M.A.F., Bellhouse, B.J., 2003. A ballistic study of micro-particle penetration to the oral mucosa. Int J Impact Eng, 28(6):581-599. 

[20] Owens, D.R., Zinman, B., Bolli, G., 2003. Alternative routes of insulin delivery. Diabetic Med, 20(11):886-898. 

[21] Sarphie, D.F., Johnson, B., Cormier, M., 1997. Bioavailability following transdermal powdered delivery (TPD) of radiolabeled inulin to hairless guinea pigs. J Control Release, 47(1):61-69. 

[22] Shaha, D., Agrawala, V., Parikh, R., 2010. Noninvasive insulin delivery system: a review. Int J Appl Pharm, 2(1):35-40. 

[23] Sivakumar, S.M., Safhi, M.M., Kannadasan, M., 2011. Vaccine adjuvants—current status and prospects on controlled release adjuvancity. Saudi Pharm J, 19(4):197-206. 

[24] Torchilin, V.P., 2005. Recent advances with liposomes as pharmaceutical carriers. Nat Rev Drug Discov, 4(2):145-160. 

[25] Trehan, A., Asgar, A.H., 1998. Recent approaches in insulin delivery. Drug Dev Ind Pharm, 24(7):589-597. 

[26] Wang, J.B., Zhou, X., Jin, C., 2006. Progress in the research of powder needle-free injection technology. Pharm J Chin PLA, (in Chinese),22(4):292-295. 

[27] Wang, J.B., Zhou, X., Li, W., 2007. Bioavailability and influencing factors of self-designed powder needleless injection system on rabbits. Acta Pharm Sin, (in Chinese),42(4):424-428. 

[28] Wang, J.B., Zhou, X., Wang, Z.W., 2009. Effect of tetanus toxoid powder needleless injection on the concentration of serum antibody IgG in mice. Acta Pharm Sin, (in Chinese),44(12):1406-1409. 

[29] Xu, H.B., Huang, K.X., Zhu, Y.S., 2002. Hypoglycaemic effect of a novel insulin buccal formulation on rabbits. Pharmacol Res, 46(5):459-467. 

[30] Zhou, X., Jin, C., Wang, J.B., 2006. Pilot study of needle-free powder jet injection drug delivery system. Pharm J Chin PLA, (in Chinese),22(2):88-90. 

[31] Ziegler, A.S., 2008. Needle-free delivery of powdered protein vaccines: a new and rapidly developing technique. J Pharm Innov, 3(3):204-213. 

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