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CLC number: X173

On-line Access: 2014-09-18

Received: 2014-04-18

Revision Accepted: 2014-09-17

Crosschecked: 2015-01-07

Cited: 11

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Citations:  Bibtex RefMan EndNote GB/T7714


Dan Liu


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.2 P.123-130


Lead accumulation and tolerance of Moso bamboo (Phyllostachys pubescens) seedlings: applications of phytoremediation

Author(s):  Dan LIU, Song LI, Ejazul ISLAM, Jun-ren CHEN, Jia-sen WU, Zheng-qian YE, Dan-li PENG, Wen-bo YAN, Kou-ping LU

Affiliation(s):  Key Laboratory of Soil Contamination Bioremediation of Zhejiang Province, School of Environmental and Resource Sciences, Zhejiang A & F University, Lin’an 311300, China; more

Corresponding email(s):   liudan7812@aliyun.com

Key Words:  Moso bamboo, Pb, Phytoremediation, Scanning electron microscopy, Transmission electron microscopy

Dan LIU, Song LI, Ejazul ISLAM, Jun-ren CHEN, Jia-sen WU, Zheng-qian YE, Dan-li PENG, Wen-bo YAN, Kou-ping LU. Lead accumulation and tolerance of Moso bamboo (Phyllostachys pubescens) seedlings: applications of phytoremediation[J]. Journal of Zhejiang University Science B, 2015, 16(2): 123-130.

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author="Dan LIU, Song LI, Ejazul ISLAM, Jun-ren CHEN, Jia-sen WU, Zheng-qian YE, Dan-li PENG, Wen-bo YAN, Kou-ping LU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Lead accumulation and tolerance of Moso bamboo (Phyllostachys pubescens) seedlings: applications of phytoremediation
%A Dan LIU
%A Song LI
%A Ejazul ISLAM
%A Jun-ren CHEN
%A Jia-sen WU
%A Zheng-qian YE
%A Dan-li PENG
%A Wen-bo YAN
%A Kou-ping LU
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 2
%P 123-130
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400107

T1 - Lead accumulation and tolerance of Moso bamboo (Phyllostachys pubescens) seedlings: applications of phytoremediation
A1 - Dan LIU
A1 - Song LI
A1 - Ejazul ISLAM
A1 - Jun-ren CHEN
A1 - Jia-sen WU
A1 - Zheng-qian YE
A1 - Dan-li PENG
A1 - Wen-bo YAN
A1 - Kou-ping LU
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 2
SP - 123
EP - 130
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400107

A hydroponics experiment was aimed at identifying the lead (pb) tolerance and phytoremediation potential of moso bamboo (Phyllostachys pubescens) seedlings grown under different pb treatments. Experimental results indicated that at the highest pb concentration (400 μmol/L), the growth of bamboo seedlings was inhibited and pb concentrations in leaves, stems, and roots reached the maximum of 148.8, 482.2, and 4282.8 mg/kg, respectively. scanning electron microscopy revealed that the excessive pb caused decreased stomatal opening, formation of abundant inclusions in roots, and just a few inclusions in stems. The ultrastructural analysis using transmission electron microscopy revealed that the addition of excessive pb caused abnormally shaped chloroplasts, disappearance of endoplasmic reticulum, shrinkage of nucleus and nucleolus, and loss of thylakoid membranes. Although ultrastructural analysis revealed some internal damage, even the plants exposed to 400 µmol/L pb survived and no visual pb toxicity symptoms such as necrosis and chlorosis were observed in these plants. Even at the highest pb treatment, no significant difference was observed for the dry weight of stem compared with controls. It is suggested that use of moso bamboo as an experimental material provides a new perspective for remediation of heavy metal contaminated soil owing to its high metal tolerance and greater biomass.


方法:根系形态分析(图3)、植物重金属含量分析 (表1)、扫描电镜分析(图4)和透射电镜分析(图5)。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]An, Y.J., Kim, Y.M., Kwon, T.I., et al., 2004. Combined effect of copper, cadmium, and lead upon Cucumis sativus growth and bioaccumulation. Sci. Total Environ., 326(1-3):85-93.

[2]Audet, P., Charest, C., 2007. Heavy metal phytoremediation from a meta-analytical perspective. Environ. Pollut., 147(1):231-237.

[3]Chen, H., Hong, W., Wei, L., et al., 1998. Study on biomass and productivity of Phyllostachys heterocycala cv. Pubescens forest in the north of Fujian. Sci. Silvae Sin., 34(S1):60-64 (in Chinese).

[4]Chen, X.G., Zhang, X.Q., Zhang, Y.P., et al., 2009. Changes of carbon stocks in bamboo stands in China during 100 years. Forest Ecol. Manag., 258(7):1489-1496.

[5]Cho-Ruk, K., Kurukote, J., Supprung, P., 2006. Perennial plants in the phytoremediation of lead-contaminated soils. Biotechnology, 5(1):1-4.

[6]Cobbett, C.S., 2000. Phytochelatins and their roles in heavy metal detoxification. Plant Physiol., 123(3):825-832.

[7]Dos Santos Utmazian, M.N., Wieshammer, G., Vega, R., et al., 2007. Hydroponic screening for metal resistance and accumulation of cadmium and zinc in twenty clones of willows and poplars. Environ. Pollut., 148(1):155-165.

[8]Epelde, L., Hernández-Allica, J., Becerril, J.M., et al., 2008. Effects of chelates on plants and soil microbial community: comparison of EDTA and EDDS for lead phytoextraction. Sci. Total Environ., 401(1-3):21-28.

[9]Fan, K.C., Hsi, H.C., Chen, C.W., et al., 2011. Cadmium accumulation and tolerance of mahogany (Swietenia macrophylla) seedlings for phytoextraction applications. J. Environ. Manag., 92(10):2818-2822.

[10]Hall, J.L., 2002. Cellular mechanisms for heavy metal detoxification and tolerance. J. Exp. Bot., 53(366):1-11.

[11]He, B., Yang, X.E., Ni, W.Z., et al., 2001. Sedum alfredii: a new lead-accumulating ecotype. Acta Bot. Sin., 44(11):1365-1370 (in Chinese).

[12]Islam, E., Yang, X.E., Li, T.Q., et al., 2007. Effect of Pb toxicity on root morphology, physiology and ultrastructure in the two ecotypes of Elsholtzia argyi. J. Hazard. Mater., 147(3):806-816.

[13]Krämer, U., 2010. Metal hyperaccumulation in plants. Annu. Rev. Plant Biol., 61(1):517-534.

[14]Li, S.L., Wang, F.P., Ru, M., et al., 2014. Cadmium tolerance and accumulation of Elsholtzia argyi origining from a zinc/lead mining site—a hydroponics experiment. Int. J. Phytoremediat., 16(12):1257-1267.

[15]López-Millán, A.F., Sagardoy, R., Solanas, M., et al., 2009. Cadmium toxicity in tomato (Lycopersicon esculentum) plants grown in hydroponics. Environ. Exp. Bot., 65(2-3):376-385.

[16]Macnair, M.R., 1993. The genetics of metal tolerance in vascular plants. New Phytol., 124(4):541-559.

[17]Nada, E., Ferjani, B.A., Ali, R., et al., 2007. Cadmium-induced growth inhibition and alteration of biochemical parameters in almond seedlings grown in solution culture. Acta Physiol. Plant., 29(1):57-62.

[18]Patra, M., Bhowmik, N., Bandopadhyay, B., et al., 2004. Comparison of mercury, lead and arsenic with respect to genotoxic effects on plant systems and the development of genetic tolerance. Environ. Exp. Bot., 52(3):199-223.

[19]Piechalak, A., Tomaszewska, B., Baralkiewicz, D., et al., 2002. Accumulation and detoxification of lead ions in legumes. Phytochemistry, 60(2):153-162.

[20]Pulford, I., Watson, C., 2003. Phytoremediation of heavy metal-contaminated land by trees—a review. Environ. Int., 29(4):529-540.

[21]Rascio, N., Navari-Izzo, F., 2011. Heavy metal hyperaccumulating plants: How and why do they do it? And what makes them so interesting? Plant Sci., 180(2):169-181.

[22]Rudakova, E.V., Karakis, K.D., Sidorshina, E.T., 1988. The role of plant cell walls in uptake and accumulation of metal ions. Fiziol. Biochim. Kult. Rast., 20(1):3-12.

[23]Ruley, A.T., Sharma, N.C., Sahi, S.V., et al., 2006. Effects of lead and chelators on growth, photosynthetic activity and Pb uptake in Sesbania drummondii grown in soil. Environ. Pollut., 144(1):11-18.

[24]Shao, J.F., Gui, R.Y., Ji, H.B., et al., 2011. A preliminary study on establishment of hydroponic culture system for Phyllostachys pubescens seedlings. J. Zhejiang A & F Univ., 28(1):86-94 (in Chinese).

[25]Sharma, P., Dubey, R.S., 2005. Lead toxicity in plants. Braz. J. Plant Physiol., 17(1):35-52.

[26]Singh, R.P., Tripathi, R.D., Sinha, S., et al., 1997. Response of higher plants to lead contaminated environment. Chemosphere, 34(11):2467-2493.

[27]Sinha, S., Gupta, A.K., Bhatt, K., 2007. Uptake and translocation of metals in fenugreek grown on soil amended with tannery sludge: involvement of antioxidants. Ecotoxicol. Environ. Saf., 67(2):267-277.

[28]Tandy, S., Schulin, R., Nowack, B., 2006. The influence of EDDS on the uptake of heavy metals in hydroponically grown sunflowers. Chemosphere, 62(9):1454-1463.

[29]Verbruggen, N., Hermans, C., Schat, H., 2009. Molecular mechanisms of metal hyperaccumulation in plants. New Phytol., 181(4):759-776.

[30]Wang, B., Wei, W.J., Liu, C.J., et al., 2013. Biomass and carbon stock in Moso bamboo forests in subtropical China: characteristics and implications. J. Trop. For. Sci., 25(1):137-148 (in Malay).

[31]Wu, J.S., Yu, Y.W., Zhu, Z., et al., 2002. Studies on the biomass of different forests in Huzhou city. J. Jiangsu Forest. Sci. Technol., 29(4):22-24 (in Chinese).

[32]Xi, X.Y., Liu, M.Y., Huang, Y., et al., 2010. Response of flue-cured tobacco plants to different concentration of lead or cadmium. 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, p.1-4.

[33]Xu, Y., Wong, M., Yang, J., et al., 2011. Dynamics of carbon accumulation during the fast growth period of bamboo plant. Bot. Rev., 77(3):287-295.

[34]Zaier, H., Ghnaya, T., Lakhdar, A., et al., 2010. Comparative study of Pb-phytoextraction potential in Sesuvium portulacastrum and Brassica juncea: tolerance and accumulation. J. Hazard. Mater., 183(1-3):609-615.

[35]Zhang, L., Ye, Z.Q., Li, T.Q., et al., 2006. Studies on soil microbial activity in areas contaminated by tailings from Pb, Zn mine. J. Soil Water Conserv., 20(3):136-140 (in Chinese).

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