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Received: 2016-05-31

Revision Accepted: 2016-08-26

Crosschecked: 2017-02-01

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Yong-liang Jiang


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.3 P.239-248


Effects of electroacupuncture at 2 and 100 Hz on rat type 2 diabetic neuropathic pain and hyperalgesia-related protein expression in the dorsal root ganglion

Author(s):  Xiao-fen He, Jun-jun Wei, Sheng-yun Shou, Jian-qiao Fang, Yong-liang Jiang

Affiliation(s):  Department of Neurobiology and Acupuncture Research, the Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China; more

Corresponding email(s):   fangjianqiao7532@163.com, jyl2182@126.com

Key Words:  Electroacupuncture, Type 2 diabetic neuropathic pain, Dorsal root ganglion, P2X3 receptor, Calcitonin gene-related peptide

Xiao-fen He, Jun-jun Wei, Sheng-yun Shou, Jian-qiao Fang, Yong-liang Jiang. Effects of electroacupuncture at 2 and 100 Hz on rat type 2 diabetic neuropathic pain and hyperalgesia-related protein expression in the dorsal root ganglion[J]. Journal of Zhejiang University Science B, 2017, 18(3): 239-248.

@article{title="Effects of electroacupuncture at 2 and 100 Hz on rat type 2 diabetic neuropathic pain and hyperalgesia-related protein expression in the dorsal root ganglion",
author="Xiao-fen He, Jun-jun Wei, Sheng-yun Shou, Jian-qiao Fang, Yong-liang Jiang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of electroacupuncture at 2 and 100 Hz on rat type 2 diabetic neuropathic pain and hyperalgesia-related protein expression in the dorsal root ganglion
%A Xiao-fen He
%A Jun-jun Wei
%A Sheng-yun Shou
%A Jian-qiao Fang
%A Yong-liang Jiang
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 3
%P 239-248
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600247

T1 - Effects of electroacupuncture at 2 and 100 Hz on rat type 2 diabetic neuropathic pain and hyperalgesia-related protein expression in the dorsal root ganglion
A1 - Xiao-fen He
A1 - Jun-jun Wei
A1 - Sheng-yun Shou
A1 - Jian-qiao Fang
A1 - Yong-liang Jiang
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 3
SP - 239
EP - 248
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600247

Objective: To investigate the analgesic effects of electroacupuncture (EA) at 2 and 100 Hz on type 2 diabetic neuropathic pain (DNP) and on the expressions of the p2X3 receptor and calcitonin gene-related peptide (CGRP) in the dorsal root ganglion (DRG). Methods: Rat type 2 DNP was induced by a high calorie and high sugar diet fed for 7 weeks, plus a single intraperitoneal injection of streptozotocin (STZ) after 5 weeks. EA at 2 and 100 Hz was carried out once every day after 7 weeks for 7 consecutive days. Body weight, serum fasting insulin (FINS), fasting blood glucose (FBG), insulin sensitivity index (ISI), and paw withdrawal latency (PWL) were measured. The expressions of L4–L6 DRG p2X3 receptors and CGRP were assessed by immunofluorescence. Results: Type 2 DNP was successfully induced as shown by the increased body weight, FINS, and FBG, as well as the reduced ISI and PWL. Expressions of p2X3 receptors and CGRP in L4–L6 DRGs increased. EA at both 2 and 100 Hz relieved type 2 DNP, but the analgesic effect of EA was stronger at 2 Hz. p2X3 receptor expression decreased in L4–L6 DRGs following EA at 2 Hz and in L5 and L6 DRGs following EA at 100 Hz. EA at both 2 and 100 Hz down-regulated CGRP overexpression in L4–L6 DRGs. Conclusions: These findings indicate that EA at 2 Hz is a good option for the management of type 2 DNP. The EA effect may be related to its down-regulation of the overexpressions of the DRG p2X3 receptors and CGRP in this condition.

研究2 Hz和100 Hz电针对2型糖尿病神经痛大鼠的镇痛作用以及对背根神经节致痛相关蛋白表达的影响

目的:研究2和100 Hz电针对2型糖尿病神经痛(DNP)大鼠的镇痛作用,以及对DNP大鼠背根神经节(DRG)上P2X3受体和降钙素相关基因肽(CGRP)表达的影响。
创新点:本研究首次选用2型DNP大鼠模型来研究电针治疗DNP的优势频率。研究证明2 Hz电针对2型DNP大鼠的镇痛作用以及对DRG上高表达的P2X3抑制作用优于100 Hz,为电针治疗DNP及其频率选择提供科学依据和阐释。
方法:将雄性SD大鼠分为对照组(腹腔注射柠檬酸钠缓冲液)和模型组(高脂高糖饲养联合腹腔注射 35 mg/kg链脲佐菌素(STZ))。模型组根据采用不同的电针频率分为以下三组:DNP组、DNP+2 Hz电针组、DNP+100 Hz电针组。采用足底测试仪检测大鼠足跖热痛阈(PWL),用酶联免疫吸附测定(ELISA)试剂盒检测大鼠空腹胰岛素(FINS)含量,用免疫荧光法检测DRG上的P2X3受体和CGRP的表达水平。
结论:2和100 Hz电针对2型DNP均有明显的镇痛作用,且2 Hz电针对2型DNP的镇痛作用优于 100 Hz电针(图2)。免疫荧光实验结果显示,2和100 Hz电针均能明显下调DRG中P2X3受体和CGRP的高表达(图3和4),且2 Hz电针对DRG中P2X3受体的抑制作用优于100 Hz电针(图3)。综上所述,2 Hz电针是治疗2型DNP较为理想的治疗手段。


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