CLC number: R733.7
On-line Access: 2015-07-03
Received: 2014-08-26
Revision Accepted: 2015-01-28
Crosschecked: 2015-06-16
Cited: 4
Clicked: 5019
Bo Wang, Sheng-yun Lin, Ying-ying Shen, Li-qiang Wu, Zhen-zhen Chen, Jing Li, Zhi Chen, Wen-bin Qian, Jian-ping Jiang. Pure total flavonoids from Citrus paradisi Macfadyen act synergistically with arsenic trioxide in inducing apoptosis of Kasumi-1 leukemia cells in vitro[J]. Journal of Zhejiang University Science B, 2015, 16(7): 580-585.
@article{title="Pure total flavonoids from Citrus paradisi Macfadyen act synergistically with arsenic trioxide in inducing apoptosis of Kasumi-1 leukemia cells in vitro",
author="Bo Wang, Sheng-yun Lin, Ying-ying Shen, Li-qiang Wu, Zhen-zhen Chen, Jing Li, Zhi Chen, Wen-bin Qian, Jian-ping Jiang",
journal="Journal of Zhejiang University Science B",
volume="16",
number="7",
pages="580-585",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400234"
}
%0 Journal Article
%T Pure total flavonoids from Citrus paradisi Macfadyen act synergistically with arsenic trioxide in inducing apoptosis of Kasumi-1 leukemia cells in vitro
%A Bo Wang
%A Sheng-yun Lin
%A Ying-ying Shen
%A Li-qiang Wu
%A Zhen-zhen Chen
%A Jing Li
%A Zhi Chen
%A Wen-bin Qian
%A Jian-ping Jiang
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 7
%P 580-585
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400234
TY - JOUR
T1 - Pure total flavonoids from Citrus paradisi Macfadyen act synergistically with arsenic trioxide in inducing apoptosis of Kasumi-1 leukemia cells in vitro
A1 - Bo Wang
A1 - Sheng-yun Lin
A1 - Ying-ying Shen
A1 - Li-qiang Wu
A1 - Zhen-zhen Chen
A1 - Jing Li
A1 - Zhi Chen
A1 - Wen-bin Qian
A1 - Jian-ping Jiang
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 7
SP - 580
EP - 585
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400234
Abstract: To investigate the potential effects of pure total flavonoid compounds (PTFCs) from Citrus paradisi Macfadyen separately or combined with arsenic trioxide on the proliferation of human myeloid leukemia cells and the mechanisms underlying the action of PTFCs. The effects of PTFCs separately or combined with arsenic trioxide on the proliferation and apoptosis of leukemia cells were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), fluorescence microscopy, and flow cytometry. Their effects on the expression levels of apoptosis-related regulators were determined by Western blot assay. PTFCs combined with arsenic trioxide significantly inhibited the growth of Kasumi-1 cells, and apoptosis was confirmed by flow cytometry analysis. Hoechst 33258 staining showed more significant morphological changes and more apoptosis following the combined treatment. Western blots showed changes in the expression of genes for poly ADP-ribose polymerase (PARP), caspase 3/9, and P65. The results indicated that PTFCs separately or combined with arsenic trioxide inhibited proliferation of leukemia cells in vitro and induced their apoptosis by modulating the expression of apoptosis-related regulator genes.
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