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Journal of Zhejiang University SCIENCE B 1998 Vol.-1 No.-1 P.

http://doi.org/10.1631/jzus.B2200048


CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells


Author(s):  Xiajing LI, Yiyu ZHANG, Ning WANG, Zhaohu YUAN, Xiaojie CHEN, Qicong CHEN, Hui DENG, Xinxin TONG, Honglin CHEN, Yuyou DUAN, Yaming WEI

Affiliation(s):  School of Medicine, South China University of Technology, Guangzhou 510000, China; more

Corresponding email(s):   yuyouduan@scut.edu.cn

Key Words:  CircRNA.0007127, miR-513a-5p, CASP8, Apoptosis, K-562 cells


Xiajing LI, Yiyu ZHANG, Ning WANG, Zhaohu YUAN, Xiaojie CHEN, Qicong CHEN, Hui DENG, Xinxin TONG, Honglin CHEN, Yuyou DUAN, Yaming WEI. CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .

@article{title="CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells",
author="Xiajing LI, Yiyu ZHANG, Ning WANG, Zhaohu YUAN, Xiaojie CHEN, Qicong CHEN, Hui DENG, Xinxin TONG, Honglin CHEN, Yuyou DUAN, Yaming WEI",
journal="Journal of Zhejiang University Science B",
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year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200048"
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%0 Journal Article
%T CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells
%A Xiajing LI
%A Yiyu ZHANG
%A Ning WANG
%A Zhaohu YUAN
%A Xiaojie CHEN
%A Qicong CHEN
%A Hui DENG
%A Xinxin TONG
%A Honglin CHEN
%A Yuyou DUAN
%A Yaming WEI
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
%P
%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200048

TY - JOUR
T1 - CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells
A1 - Xiajing LI
A1 - Yiyu ZHANG
A1 - Ning WANG
A1 - Zhaohu YUAN
A1 - Xiaojie CHEN
A1 - Qicong CHEN
A1 - Hui DENG
A1 - Xinxin TONG
A1 - Honglin CHEN
A1 - Yuyou DUAN
A1 - Yaming WEI
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200048


Abstract: 
Background: Circular RNAs (circRNAs) are covalently closed single-stranded RNAs with multiple biological functions. CircRNA.000712 is derived from the CCR4-NOT complex subunit 2 (CNOT2), which was found to regulate tumor cell apoptosis through caspase pathway. Methods: Potential circRNA.0007127 target miRNAs were analyzed by Miranda TargetScan and RnaHybrid software, and the miRNAs with binding sites for apoptosis-related genes were screened. The roles of circRNA.0007127 and its downstream target, miR-513a-5p, were validated by qPCR, flow cytometry, mitochondrial membrane potential, immunofluorescence, western blot and CASP8 protein activity in vitro in H2O2-induced k-562 cells. The circRNA.0007127miR-513a-5p and CASP8miR-513a-5p interactions were verified by luciferase reporter assays. Results: Silencing circRNA.0007127 decreased cell apoptosis by inhibiting CASP8 pathway activation in k-562 cells. Compared with the control group, the expression of CASP8 was reduced by 2-fold, and the 43 kD fragment of CASP8 protein was significantly reduced (P≤0.05). The luciferase reporting assay showed that circRNA.0007127 combined with miR-513a-5p or CASP8, with extremely significant differences (P≤0.001). The overexpression of miR-513a-5p inhibited the gene expression level of CASP8 in a human myeloid leukemia cell model (4-fold change) and the level of a 43-kD fragment of CASP8 protein (P≤0.01). The rescue experiment showed that cotransfection with circRNA.0007127 siRNA and the miR-513a-5p inhibitor increased CASP8 gene expression and the apoptosis rate, suggesting that the miR-513a-5p inhibitor is a circRNA.0007127 siRNA antagonist. Conclusion: circRNA.0007127 regulates K-562 cell apoptosis through the miR-513a-5p/CASP8 axis, which can serve as a novel powerful molecular target for k-562 cells.

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