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CLC number: S828

On-line Access: 2015-06-08

Received: 2014-10-03

Revision Accepted: 2015-01-31

Crosschecked: 2015-05-13

Cited: 5

Clicked: 3392

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Hong-fu Zhang

http://orcid.org/0000-0001-8790-2709

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.6 P.465-478

http://doi.org/10.1631/jzus.B1400266


iTRAQ-based quantitative proteomic analysis of longissimus muscle from growing pigs with dietary supplementation of non-starch polysaccharide enzymes


Author(s):  Ji-ze Zhang, Yang Gao, Qing-ping Lu, Ren-na Sa, Hong-fu Zhang

Affiliation(s):  State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; more

Corresponding email(s):   zhanghf6565@vip.sina.com

Key Words:  Non-starch polysaccharide enzymes (NSPEs), Longissimus muscle, Proteomics, Growing pigs


Ji-ze Zhang, Yang Gao, Qing-ping Lu, Ren-na Sa, Hong-fu Zhang. iTRAQ-based quantitative proteomic analysis of longissimus muscle from growing pigs with dietary supplementation of non-starch polysaccharide enzymes[J]. Journal of Zhejiang University Science B, 2015, 16(6): 465-478.

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author="Ji-ze Zhang, Yang Gao, Qing-ping Lu, Ren-na Sa, Hong-fu Zhang",
journal="Journal of Zhejiang University Science B",
volume="16",
number="6",
pages="465-478",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400266"
}

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%T iTRAQ-based quantitative proteomic analysis of longissimus muscle from growing pigs with dietary supplementation of non-starch polysaccharide enzymes
%A Ji-ze Zhang
%A Yang Gao
%A Qing-ping Lu
%A Ren-na Sa
%A Hong-fu Zhang
%J Journal of Zhejiang University SCIENCE B
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%P 465-478
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400266

TY - JOUR
T1 - iTRAQ-based quantitative proteomic analysis of longissimus muscle from growing pigs with dietary supplementation of non-starch polysaccharide enzymes
A1 - Ji-ze Zhang
A1 - Yang Gao
A1 - Qing-ping Lu
A1 - Ren-na Sa
A1 - Hong-fu Zhang
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 6
SP - 465
EP - 478
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400266


Abstract: 
non-starch polysaccharide enzymes (NSPEs) have long been used in the feed production of monogastric animals to degrade non-starch polysaccharide to oligosaccharides and promote growth performance. However, few studies have been conducted on the effect of such enzymes on skeletal muscle in monogastric animals. To elucidate the mechanism of the effect of NSPEs on skeletal muscle, an isobaric tag for relative and absolute quantification (iTRAQ) for differential proteomic quantitation was applied to investigate alterations in the proteome in the longissimus muscle (LM) of growing pigs after a 50-d period of supplementation with 0.6% NSPEs in the diet. A total of 51 proteins were found to be differentially expressed in the LM between a control group and the NSPE group. Functional analysis of the differentially expressed protein species showed an increased abundance of proteins related to energy production, protein synthesis, muscular differentiation, immunity, oxidation resistance and detoxification, and a decreased abundance of proteins related to inflammation in the LM of the pigs fed NSPEs. These findings have important implications for understanding the mechanisms whereby dietary supplementation with NSPEs enzymes can promote growth performance and improve muscular metabolism in growing pigs.

基于iTRAQ定量蛋白质组学技术分析日粮中添加非淀粉多糖酶对生长猪背最长肌中蛋白表达影响

目的:通过日粮中添加非淀粉多糖酶,运用同位素标记相对和绝对定量技术(iTRAQ技术)分析非淀粉多糖酶对生长猪背最长肌蛋白质表达有何影响,为饲料中添加非淀粉多糖酶提供理论基础。
创新点:采用iTRAQ定量蛋白质组学技术,通过对生长猪背最长肌蛋白质表达进行高通量分析,发现日粮中添加非淀粉多糖酶可影响许多功能蛋白表达,从分子水平阐述了其发挥作用的机理。
方法:将体重约39kg生长猪(48头)随机分为两个处理,每个处理4个重复,每个重复6头猪。对照组饲喂基础日粮,试验组在基础日粮中添加0.6%非淀粉多糖酶。50天试验期后,每个重复屠宰1头猪(n=8),取背最长肌,通过iTRAQ定量蛋白质组学技术分析肌肉组织中差异蛋白表达。
结论:iTRAQ定量蛋白质组学分析结果显示,试验组与对照组相比,共发现51个差异蛋白,其中38个可进行生物学功能定位(图1,表6)。差异表达蛋白中与能量生成、蛋白质合成、肌肉分化、免疫、抗氧化和解毒相关蛋白表达量上调,而与炎症反应相关蛋白表达量下调。综上所述,生长猪日粮中添加非淀粉多糖酶不仅可改善生产性能,同时还可调节肌肉中诸多代谢功能。

关键词:非淀粉多糖酶;背最长肌;蛋白质组学;生长猪

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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