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On-line Access: 2015-10-03

Received: 2015-02-26

Revision Accepted: 2015-05-07

Crosschecked: 2015-09-13

Cited: 3

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Citations:  Bibtex RefMan EndNote GB/T7714


Wen Zong


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.10 P.857-864


Estradiol plays a role in regulating the expression of lysyl oxidase family genes in mouse urogenital tissues and human Ishikawa cells

Author(s):  Wen Zong, Yan Jiang, Jing Zhao, Jian Zhang, Jian-gang Gao

Affiliation(s):  Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China

Corresponding email(s):   zhaojing@sdu.edu.cn, zhj8226@sdu.edu.cn

Key Words:  Estradiol, Lysyl oxidase family genes, TGF-β, signal pathway

Wen Zong, Yan Jiang, Jing Zhao, Jian Zhang, Jian-gang Gao. Estradiol plays a role in regulating the expression of lysyl oxidase family genes in mouse urogenital tissues and human Ishikawa cells[J]. Journal of Zhejiang University Science B, 2015, 16(10): 857-864.

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author="Wen Zong, Yan Jiang, Jing Zhao, Jian Zhang, Jian-gang Gao",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Estradiol plays a role in regulating the expression of lysyl oxidase family genes in mouse urogenital tissues and human Ishikawa cells
%A Wen Zong
%A Yan Jiang
%A Jing Zhao
%A Jian Zhang
%A Jian-gang Gao
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 10
%P 857-864
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500048

T1 - Estradiol plays a role in regulating the expression of lysyl oxidase family genes in mouse urogenital tissues and human Ishikawa cells
A1 - Wen Zong
A1 - Yan Jiang
A1 - Jing Zhao
A1 - Jian Zhang
A1 - Jian-gang Gao
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 10
SP - 857
EP - 864
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500048

The lysyl oxidase (LOX) family encodes the copper-dependent amine oxidases that play a key role in determining the tensile strength and structural integrity of connective tissues by catalyzing the crosslinking of elastin or collagen. Estrogen may upregulate the expression of LOX and lysyl oxidase-like 1 (LOXL1) in the vagina. The objective of this study was to determine the effect of estrogen on the expression of all LOX family genes in the urogenital tissues of accelerated ovarian aging mice and human Ishikawa cells. Mice and Ishikawa cells treated with estradiol (E2) showed increased expression of LOX family genes and transforming growth factor β1 (TGF-β;1). Ishikawa cells treated with TGF-β;1 also showed increased expression of LOX family genes. The Ishikawa cells were then treated with either E2 plus the TGF-β; receptor (TGFBR) inhibitor SB431542 or E2 alone. The expression of LOX family genes induced by E2 was reduced in the Ishikawa cells treated with TGFBR inhibitor. Our results showed that E2 increased the expression of the LOX family genes, and suggest that this induction may be mediated by the TGF-β; signal pathway. E2 may play a role in regulating the expression of LOX family genes.




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