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CLC number: S123

On-line Access: 2016-06-03

Received: 2015-04-15

Revision Accepted: 2015-11-30

Crosschecked: 2016-05-11

Cited: 3

Clicked: 2772

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Xia-ping Fu

http://orcid.org/0000-0002-4156-668X

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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.6 P.484-492

http://doi.org/10.1631/jzus.B1500086


Investigation of absorption and scattering characteristics of kiwifruit tissue using a single integrating sphere system


Author(s):  Zhen-huan Fang, Xia-ping Fu, Xue-ming He

Affiliation(s):  College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   fuxp@zju.edu.cn

Key Words:  Optical properties, Integrating sphere, Inverse adding-doubling, Kiwifruit


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Zhen-huan Fang, Xia-ping Fu, Xue-ming He. Investigation of absorption and scattering characteristics of kiwifruit tissue using a single integrating sphere system[J]. Journal of Zhejiang University Science B, 2016, 17(6): 484-492.

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Abstract: 
For a quantitative understanding of light interaction with fruit tissue, it is critical to obtain two fundamental parameters: the absorption coefficient and the scattering coefficient of the tissue. This study was to investigate the optical properties of kiwifruit tissue at the wavelength of 632.8 nm. The total reflectance and total transmittance of kiwifruit tissue from three parts (including the flesh part, the seed part, and the seed-base part) were measured using a single integrating sphere system. Based on the measured spectral signals, the absorption coefficient μa and the reduced scattering coefficient μs' of kiwifruit tissue were calculated using the inverse adding-doubling (IAD) method. Phantoms made from Intralipid 20% and India ink as well as a Biomimic solid phantom were used for system validation. The mean values of μa and μs' of different parts of the kiwifruit were 0.031–0.308 mm−1 and 0.120–0.946 mm−1, respectively. The results showed significant differences among the μa and μs' of the three parts of the kiwifruit. The results of this study confirmed the importance of studying the optical properties for a quantitative understanding of light interaction with fruit tissue. Further investigation of fruit optical properties will be extended to a broader spectral region and different kinds of fruits.

基于单积分球系统的猕猴桃组织吸收和散射特性

目的:开展水果组织的光传输特性检测分析,以猕猴桃组织为例探讨其在632.8 nm波长的光传输特性。
创新点:开发基于单积分球的水果组织光传输特性自动检测系统,验证其可靠性并应用于水果组织的分析,并获得猕猴桃组织不同部位在632.8 nm的吸收和散射系数。
方法:利用所搭建的单积分球系统,获取三个不同部位(果肉、种子和种子基座)组织的全反射和全透射信息,测量各个组织切片的厚度及折射率。运用逆倍增算法计算得到各组织样本的吸收系数μa和约化散射系数μs’,并根据计算所得结果对猕猴桃不同组织的光传输特性进行比较分析。
结论:本实验结果测得猕猴桃不同部位组织的吸收系数和散射系数分别为0.031~0.308 mm−1和0.120~0.946 mm−1,并显示猕猴桃不同部位组织的吸收和散射特性具有显著差异。种子部位受随机分散的种子的影响较大,组织较均匀的果肉和种子基座部位结果证实散射系数大于吸收系数,符合生物组织高散射介质特性。这些结果说明了定量测定组织的光传输特性参数对深入研究光与水果组织相互作用的重要性。

关键词:光学特性;积分球;逆倍增算法;猕猴桃

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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