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CLC number: S831.5

On-line Access: 2015-12-04

Received: 2015-06-28

Revision Accepted: 2015-09-14

Crosschecked: 2015-11-18

Cited: 5

Clicked: 4023

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Hua-li Li

http://orcid.org/0000-0003-2210-2780

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.12 P.1019-1026

http://doi.org/10.1631/jzus.B1500160


Long-term effects of oral tea polyphenols and Lactobacillus brevis M8 on biochemical parameters, digestive enzymes, and cytokines expression in broilers


Author(s):  Hua-li Li, Zong-jun Li, Zhong-shan Wei, Ting Liu, Xiao-zuo Zou, Yong Liao, Yu Luo

Affiliation(s):  Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; more

Corresponding email(s):   hnlizongjun@163.com

Key Words:  Tea polyphenols, Lactobacillus brevis, Cytokines, Broilers


Hua-li Li, Zong-jun Li, Zhong-shan Wei, Ting Liu, Xiao-zuo Zou, Yong Liao, Yu Luo. Long-term effects of oral tea polyphenols and Lactobacillus brevis M8 on biochemical parameters, digestive enzymes, and cytokines expression in broilers[J]. Journal of Zhejiang University Science B, 2015, 16(12): 1019-1026.

@article{title="Long-term effects of oral tea polyphenols and Lactobacillus brevis M8 on biochemical parameters, digestive enzymes, and cytokines expression in broilers",
author="Hua-li Li, Zong-jun Li, Zhong-shan Wei, Ting Liu, Xiao-zuo Zou, Yong Liao, Yu Luo",
journal="Journal of Zhejiang University Science B",
volume="16",
number="12",
pages="1019-1026",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500160"
}

%0 Journal Article
%T Long-term effects of oral tea polyphenols and Lactobacillus brevis M8 on biochemical parameters, digestive enzymes, and cytokines expression in broilers
%A Hua-li Li
%A Zong-jun Li
%A Zhong-shan Wei
%A Ting Liu
%A Xiao-zuo Zou
%A Yong Liao
%A Yu Luo
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 12
%P 1019-1026
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500160

TY - JOUR
T1 - Long-term effects of oral tea polyphenols and Lactobacillus brevis M8 on biochemical parameters, digestive enzymes, and cytokines expression in broilers
A1 - Hua-li Li
A1 - Zong-jun Li
A1 - Zhong-shan Wei
A1 - Ting Liu
A1 - Xiao-zuo Zou
A1 - Yong Liao
A1 - Yu Luo
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 12
SP - 1019
EP - 1026
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500160


Abstract: 
This study investigates the long-term effects of oral tea polyphenols (TPs) and Lactobacillus brevis M8 (LB) on biochemical parameters, digestive enzymes, and cytokines expression in broilers. In experiment 1, 240 broiler chickens were selected to investigate the effects of 0.06 g/kg body weight (BW) TP and 1.0 ml/kg BW LB on broilers; in experiment 2, 180 broiler chickens were assigned randomly to three groups to investigate the effects of different dosages of TP (0.03, 0.06, and 0.09 g/kg BW) combined with 1.0 ml/kg BW LB on broilers; in experiment 3, 180 broiler chickens were assigned randomly to three groups to investigate the effects of different dosages of LB (0.5, 1.0, and 1.5 ml/kg BW) combined with 0.06 g/kg BW TP on broilers. The results showed that TP and LB affected serum biochemical parameters, and TP reduced serum cholesterol (CHO) and low-density lipoprotein cholesterol (LDL-C) abundances in a dosage-dependent manner (P<0.05) on Day 84. Meanwhile, broilers fed a diet supplemented with TP or LB had a lower intestinal lipase activity on Day 84 compared with the control group (P<0.05). Middle and high dosages of TP increased pancreatic lipase and proventriculus pepsin activities (P<0.05). Also middle and high dosages of LB significantly enhanced pancreatic lipase activity (P<0.05), while high LB supplementation inhibited intestinal trypsase (P<0.05) on Day 84. Furthermore, both TP and LB reduced intestinal cytokine expression and nuclear factor-κ B (NF-κB) mRNA level on Days 56 and 84. In conclusion, long-term treatment of TP and LB improved lipid metabolism and digestive enzymes activities, and affected intestinal inflammatory status, which may be associated with the NF-κB signal.

茶多酚和乳酸菌影响肉鸡血液生化、消化酶以及肠道细胞因子表达的研究

目的:茶多酚和乳酸菌在动物营养上的作用已经得到广泛的验证,但是关于二者联合作用的研究鲜有报道。本文采用肉鸡作为试验模型,研究了茶多酚和乳酸菌联合灌喂对肉鸡血液生化、消化酶以及肠道细胞因子表达的影响。
创新点:本研究首次采用联合灌喂茶多酚和乳酸菌,探讨了二者联合作用对肉鸡的影响,并通过模拟生产,长期观察了茶多酚和乳酸菌对肉鸡的影响。
方法:对肉仔鸡灌喂不同浓度的茶多酚和乳酸菌,在第56和84天随机屠宰取样。收集血液检查血液生化指标,并测定消化酶活性。取肠道样品提取RNA,采用反转录聚合酶链反应(RT-PCR)检测细胞因子的表达以及相关信号通路的激活。
结论:长期灌喂茶多酚和乳酸菌改善了肉鸡脂质代谢、消化酶活性以及炎症反应,其机制可能是通过影响了NF-κB信号通路。

关键词:茶多酚;乳酸菌;NF-κB;肉鸡

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[42]中文摘要

[43]题目:茶多酚和乳酸菌影响肉鸡血液生化、消化酶以及肠道细胞因子表达的研究

[44]目的:茶多酚和乳酸菌在动物营养上的作用已经得到广泛的验证,但是关于二者联合作用的研究鲜有报道.本文采用肉鸡作为试验模型,研究了茶多酚和乳酸菌联合灌喂对肉鸡血液生化、消化酶以及肠道细胞因子表达的影响.

[45]创新点:本研究首次采用联合灌喂茶多酚和乳酸菌,探讨了二者联合作用对肉鸡的影响,并通过模拟生产,长期观察了茶多酚和乳酸菌对肉鸡的影响.

[46]方法:对肉仔鸡灌喂不同浓度的茶多酚和乳酸菌,在第56和84天随机屠宰取样.收集血液检查血液生化指标,并测定消化酶活性.取肠道样品提取RNA,采用反转录聚合酶链反应(RT-PCR)检测细胞因子的表达以及相关信号通路的激活.

[47]结论:长期灌喂茶多酚和乳酸菌改善了肉鸡脂质代谢、消化酶活性以及炎症反应,其机制可能是通过影响了NF-κB信号通路.

[48]关键词:茶多酚;乳酸菌;NF-κB;肉鸡

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