Full Text:   <2614>

Summary:  <1838>

CLC number: X173; X53

On-line Access: 2016-06-03

Received: 2015-10-08

Revision Accepted: 2015-12-18

Crosschecked: 2016-05-12

Cited: 0

Clicked: 4755

Citations:  Bibtex RefMan EndNote GB/T7714


Hong-yun Peng


-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.6 P.447-454


Apigenin-7-O-β-D-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens

Author(s):  Hong-yun Peng, Xue-hong Zhang, Jin-zhong Xu

Affiliation(s):  Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Education, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   penghongyun@zju.edu.cn

Key Words:  Chromatographic separation, Copper-tolerant plant, Elsholtzia splendens, Flavonoid glycoside, Structure elucidation

Hong-yun Peng, Xue-hong Zhang, Jin-zhong Xu. Apigenin-7-O-β-D-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens[J]. Journal of Zhejiang University Science B, 2016, 17(6): 447-454.

@article{title="Apigenin-7-O-β-D-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens",
author="Hong-yun Peng, Xue-hong Zhang, Jin-zhong Xu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Apigenin-7-O-β-D-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens
%A Hong-yun Peng
%A Xue-hong Zhang
%A Jin-zhong Xu
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 6
%P 447-454
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500242

T1 - Apigenin-7-O-β-D-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens
A1 - Hong-yun Peng
A1 - Xue-hong Zhang
A1 - Jin-zhong Xu
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 6
SP - 447
EP - 454
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500242

Elsholtzia splendens (Lamiaceae) is a copper-tolerant plant species growing on copper deposits in the south of China. chromatographic separation of n-BuOH extracts from the flowering aerial biomass afforded apigenin-7-O-β-D-glycoside, using macroporous resin, Sephadex™ LH-20 gel, polyamide resin as well as preparative high-performance liquid chromatography (P-HPLC) columns. Chemical structure was elucidated using HPLC/ESI-MS (electrospray ionization-mass spectrometry), Fourier transform infrared (FTIR), and 1D- and 2D-nuclear magnetic resonance (NMR). Apigenin-7-O-β-D-glycoside could be the post-harvesting product from E. splendens biomass.


方法:以铜耐性植物海州香薷为原料,用60%乙醇溶液浸提,提取液依次使用石油醚、乙酸乙酯、饱和正丁醇萃取。实验中,对饱和正丁醇萃取层,使用D101型大孔吸附树脂粗分离提取,Sephadex™ LH-20型凝胶筛分分离,并借助半制备液相和聚酰胺树脂分离纯化,得到芹菜素糖苷单体(图1)。利用紫外光谱、液相色谱–质谱联用、红外光谱、一维和二维核磁图谱,鉴定芹菜素糖苷结构(图2~5)。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Berashvili, D.T., Alaniya, M.D., Bakuridze, A.D., et al., 2005. Apigenin glucuronide from Perilla nankinensis leaves. Chem. Nat. Compd., 41(1):97-98.

[2]Choi, E.J., Kim, G.H., 2008a. In vivo antioxidative characteristics of extracts from the aromatic herb Elsholtzia splendens. Food Sci. Biotechnol., 17(5):1128-1130.

[3]Choi, E.J., Kim, G.H., 2008b. Effect of Elsholtzia splendens extracts on the blood lipid profile and hepatotoxicity of the mice. Food Sci. Biotechnol., 17:413-416.

[4]Choi, E.J., Lee, Y.S., Kim, G.H., 2007. Antioxidative characteristics of extracts from aromatic herb Elsholtzia splendens. Food Sci. Biotechnol., 16:489-492.

[5]Choi, E.J., Kim, T., Kim, G.H., 2008. Antioxidant effects of Elsholtzia splendens extract on DMBA-induced oxidative stress in mice. Food Sci. Biotechnol., 17:1341-1344.

[6]Chung, M.S., Kim, G.H., 2010. Effects of Elsholtzia splendens and Cirsium japonicum on premenstrual syndrome. Nutr. Res. Pract., 4(4):290-294.

[7]Kikuchi, M., Matsuda, N., 1996. Flavone glycosides from Lonicera gracilipes var. glandulosa. J. Nat. Prod., 59(3):314-315.

[8]Kim, D.W., Son, K.H., Chang, H.W., et al., 2003. Anti-inflammatory activity of Elsholtzia splendens. Arch. Pharm. Res., 26:232-236.

[9]Liu, J., Chen, L., Cai, S., et al., 2012. Semisynthesis of apigenin and acacetin-7-O-β-D-glycosides from naringin and their cytotoxic activities. Carbohyd. Res., 357:41-46.

[10]Malikov, V.M., Yuldashev, M.P., 2002. Phenolic compounds of plants of the Scutellaria L. genus. Distribution, structure, and properties. Chem. Nat. Compd., 38(4):358-406.

[11]Peng, H.Y., Kroneck, P.M.H., Kupper, H., 2013. Toxicity and deficiency of copper in Elsholtzia splendens affect photosynthesis biophysics, pigments and metal accumulation. Environ. Sci. Technol., 47(12):6120-6128.

[12]Peng, H.Y., Xing, Y., Gao, L.L., et al., 2014. Simultaneous separation of apigenin, luteolin and rosmarinic acid from the aerial parts of the copper-tolerant plant Elsholtzia splendens. Environ. Sci. Pollut. Res., 21(13):8124-8132.

[13]She, G.M., Guo, Z.Q., Lv, H.N., et al., 2009. New flavonoid glycosides from Elsholtzia rugulosa Hemsl. Molecules, 14(10):4190-4196.

[14]Shen, C.C., Chang, Y.S., Ho, L.K., 1993. Nuclear magnetic resonance studies of 5,7-dihydroxy flavonoids. Phytochemistry, 34(3):843-845.

[15]Wang, H.P., Lu, J.F., Zhang, G.L., et al., 2014. Endothelium-dependent and -independent vasorelaxant actions and mechanisms induced by total flavonoids of Elsholtzia

[16]splendens in rat aortas. Environ. Toxicol. Phar., 38(2):453-459.

[17]Xie, X.J., Xu, Z.B., 1952. Elsholtzia haichouensis—an indicator of copper mine. Geol. Acta, 32:360-368 (in Chinese).

[18]Yang, A.M., Lu, R.H., Shi, Y.P., 2007. Study on flavonoids from Lagotis ramalana Batalin. Chin. Pharm. J., 19:1459-1461 (in Chinese).

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE