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Received: 2015-11-26

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 ORCID:

Betul Apaydin Yildirim

http://orcid.org/0000-0002-0361-6148

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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.6 P.501-511

http://doi.org/10.1631/jzus.B1500291


Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats


Author(s):  Betul Apaydin Yildirim, Saban Kordali, Kubra Asena Terim Kapakin, Fatih Yildirim, Esra Aktas Senocak, Serdar Altun

Affiliation(s):  Department of Biochemistry, Veterinary Faculty, Ataturk University, 25240 Erzurum, Turkey; more

Corresponding email(s):   betul_apaydin@hotmail.com

Key Words:  Antioxidants, Extract, Gentamicin, Helichrysum plicatum DC. subsp. plicatum, Nephrotoxicity, Oxidative stress


Betul Apaydin Yildirim, Saban Kordali, Kubra Asena Terim Kapakin, Fatih Yildirim, Esra Aktas Senocak, Serdar Altun. Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats[J]. Journal of Zhejiang University Science B, 2017, 18(6): 501-511.

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author="Betul Apaydin Yildirim, Saban Kordali, Kubra Asena Terim Kapakin, Fatih Yildirim, Esra Aktas Senocak, Serdar Altun",
journal="Journal of Zhejiang University Science B",
volume="18",
number="6",
pages="501-511",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500291"
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%T Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats
%A Betul Apaydin Yildirim
%A Saban Kordali
%A Kubra Asena Terim Kapakin
%A Fatih Yildirim
%A Esra Aktas Senocak
%A Serdar Altun
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500291

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T1 - Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats
A1 - Betul Apaydin Yildirim
A1 - Saban Kordali
A1 - Kubra Asena Terim Kapakin
A1 - Fatih Yildirim
A1 - Esra Aktas Senocak
A1 - Serdar Altun
J0 - Journal of Zhejiang University Science B
VL - 18
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SP - 501
EP - 511
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PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500291


Abstract: 
The aim of this study was to evaluate the possible therapeutic or protective effects of Helichrysum plicatum DC. subsp. plicatum ethanol extract (HPE) against gentamicin-induced nephrotoxicity. Thirty-six Sprague Dawley male rats weighing between 200 and 250 g were used as live material. They were formed into six groups containing 6 rats each and were allowed to adapt to laboratory conditions for 7 d. Group I: control, 5% DMSO intraperitoneal (i.p.); Group II: HPE 100 mg/(kg·d) i.p.; Group III: HPE 200 mg/(kg·d) i.p.; Group IV: gentamicin as 80 mg/(kg·d) i.p.; Group V: gentamicin as 80 mg/(kg·d) i.p.+HPE 100 mg/(kg·d) i.p.; and Group VI: gentamicin as 80 mg/(kg·d) i.p.+HPE 200 mg/(kg·d) i.p. for 8 d. Following treatment, serum, liver, and kidney tissues were used to assess blood urea nitrogen (BUN), creatinine, enzymatic and non-enzymatic antioxidants, and lipid peroxidation. gentamicin significantly increased serum BUN, creatinin, and liver and kidney levels of malondialdehyde (MDA). It also decreased the activity of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD). Treatment with the HPE 100 mg/kg reversed gentamicin-induced alterations as evidenced by decreased serum BUN and creatinin, liver and kidney oxidant marker, and tubular necrosis as well as by an increase in antioxidant enzymes. It was found that HPE 200 mg/kg significantly increased liver and kidney tissue MDA levels in nephrotoxicity in rats. As a result, these findings support the proposition that HPE in 100 mg/kg dose demonstrates in the kidney and liver as free radicals and scavenger to prevent the toxic effects of gentamicin in both the biochemical and histopathology parameters.

蜡菊乙醇提取物对庆大霉素诱导的大鼠肾毒性的影响

目的:评估蜡菊乙醇提取物(HPE)对庆大霉素诱导的肾毒性的治疗或保护作用。
方法:将36只体重200~250 g的Sprague Dawley雄性大鼠分成6组,每组6只,适应实验室条件7 d。每组处理方式不同,包括:组I,对照组,5% DMSO;组II,HPE 100 mg/(kg·d);组III,HPE 200 mg/(kg·d);组IV,庆大霉素80 mg/(kg·d);组V,庆大霉素80 mg/(kg·d)+HPE 100 mg/(kg·d);组VI,庆大霉素80 mg/(kg·d)+HPE 200 mg/(kg·d)。腹腔注射8 d后,取血清、肝和肾组织用于评估血液尿素氮(BUN)、肌酐、酶和非酶抗氧化剂和脂质过氧化。
结论:庆大霉素能显著提升血清BUN、肌酐和肝肾阳性以及丙二醛(MDA)水平,同时降低过氧化氢酶(CAT),谷胱甘肽过氧化物酶(GPx)和超氧化物歧化酶(SOD)的活性。用100 mg/kg HPE的治疗能逆转庆大霉素诱导的改变。因此,100 mg/kg HPE在肾脏和肝脏中可作为自由基和清除剂,具有缓解庆大霉素在生物化学和组织病理学上毒性的作用。

关键词:抗氧化剂;提取物;蜡菊;庆大霉素;肾毒性;氧化应激

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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