CLC number: R965
On-line Access: 2016-09-07
Received: 2016-01-16
Revision Accepted: 2016-04-10
Crosschecked: 2016-08-18
Cited: 0
Clicked: 5132
Yun-zhi Ling, Xiao-hong Li, Li Yu, Ye Zhang, Qi-sheng Liang, Xiao-di Yang, Hong-tao Wang. Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide[J]. Journal of Zhejiang University Science B, 2016, 17(9): 692-702.
@article{title="Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide",
author="Yun-zhi Ling, Xiao-hong Li, Li Yu, Ye Zhang, Qi-sheng Liang, Xiao-di Yang, Hong-tao Wang",
journal="Journal of Zhejiang University Science B",
volume="17",
number="9",
pages="692-702",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600017"
}
%0 Journal Article
%T Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide
%A Yun-zhi Ling
%A Xiao-hong Li
%A Li Yu
%A Ye Zhang
%A Qi-sheng Liang
%A Xiao-di Yang
%A Hong-tao Wang
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 9
%P 692-702
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600017
TY - JOUR
T1 - Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide
A1 - Yun-zhi Ling
A1 - Xiao-hong Li
A1 - Li Yu
A1 - Ye Zhang
A1 - Qi-sheng Liang
A1 - Xiao-di Yang
A1 - Hong-tao Wang
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 9
SP - 692
EP - 702
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600017
Abstract: Objective: To investigate the protective effects of parecoxib from oxidative stress induced by hydrogen peroxide (H2O2) in rat astrocytes in vitro. Methods: All experiments included 4 groups: (1) negative control (NC) group, without any treatment; (2) H2O2 treatment group, 100 μmol/L H2O2 treatment for 24 h; (3) and (4) parecoxib pretreatment groups, 80 and 160 μmol/L parecoxib treatment for 24 h, respectively, and then treated with 100 μmol/L H2O2. Several indices were investigated, and the expressions of bax, bcl-2, and brain-derived neurotrophic factor (BDNF) were quantified. Results: Compared to the NC group, exposure to H2O2 resulted in significant morphological changes, which could be reversed by pretreatment of parecoxib. In addition, H2O2 treatment led to loss of viability (P=0.026) and increased intracellular reactive oxygen species (ROS) levels (P<0.001), and induced apoptosis (P<0.01) in the primary astrocytes relative to the NC group. However, in the parecoxib pretreatment groups, all the above changes reversed significantly (P<0.05) as compared to the H2O2 treatment group, and were nearly unchanged when compared to the NC group. Mechanical investigation showed that dysregulated bax, bcl-2, and BDNF could be implicated in these changes. Conclusions: Our results indicated that parecoxib provided a protective effect from oxidative stress induced by exposure to H2O2.
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[24]List of electronic supplementary materials
[25]Fig. S1 Identification of primary astrocytes using GFAP staining
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