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CLC number: Q946

On-line Access: 2017-09-05

Received: 2016-06-02

Revision Accepted: 2016-10-23

Crosschecked: 2017-08-16

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Hyun-Dong Paik

http://orcid.org/0000-0001-9891-7703

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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.9 P.816-824

http://doi.org/10.1631/jzus.B1600234


Antimelanogenic effects of Inula britannica flower petal extract fermented by Lactobacillus plantarum KCCM 11613P


Author(s):  Eun-hye Park, Won-young Bae, Jae-yeon Kim, Kee-tae Kim, Hyun-dong Paik

Affiliation(s):  Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Korea; more

Corresponding email(s):   hdpaik@konkuk.ac.kr

Key Words:  Inula britannica, Flavonoid, Lactobacillus plantarum, Antioxidant, Tyrosinase inhibitor


Eun-hye Park, Won-young Bae, Jae-yeon Kim, Kee-tae Kim, Hyun-dong Paik. Antimelanogenic effects of Inula britannica flower petal extract fermented by Lactobacillus plantarum KCCM 11613P[J]. Journal of Zhejiang University Science B, 2017, 18(9): 816-824.

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author="Eun-hye Park, Won-young Bae, Jae-yeon Kim, Kee-tae Kim, Hyun-dong Paik",
journal="Journal of Zhejiang University Science B",
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number="9",
pages="816-824",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600234"
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%T Antimelanogenic effects of Inula britannica flower petal extract fermented by Lactobacillus plantarum KCCM 11613P
%A Eun-hye Park
%A Won-young Bae
%A Jae-yeon Kim
%A Kee-tae Kim
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A1 - Eun-hye Park
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A1 - Kee-tae Kim
A1 - Hyun-dong Paik
J0 - Journal of Zhejiang University Science B
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DOI - 10.1631/jzus.B1600234


Abstract: 
The inhibitory effects of Lactobacillus plantarum-fermented and non-fermented Inula britannica extracts on the tyrosinase activity were comparatively investigated to examine whether and how they improve the whitening activity, and the contents of total flavonoids and polyphenolics as bioactive compounds were determined. The skin whitening activity using in vitro or ex vivo tyrosinase and L-3,4-dihydroxyphenylalanine (L-DOPA) staining was examined. The total flavonoid content (TFC) was increased by 13.4% after 72 h-fermentation. The viabilities of the B16F10 cells treated with the fermented and non-fermented control extracts were 100.26% and 92.15% at 500 µg/ml, respectively. In addition, the inhibition of tyrosinase activity was increased by the fermented samples from 29.33% to 41.74% following fermentation for up to 72 h. The tyrosinase activity of the untreated control group was increased to 145.69% in B16F10 cells. The results showed that I. britannica fermented by L. plantarum dose-dependently inhibited tyrosinase activity, which was stimulated by α-melanocyte stimulating hormone. These results suggest that lactic fermented I. britannica extracts can be used as effective skin-whitening materials.

植物乳杆菌KCCM 11613P发酵的旋覆花花瓣提取物的抗黑素生成的作用

目的:研究植物乳杆菌发酵对旋覆花花瓣提取物对酪氨酸酶的抑制效果,为开发旋覆花提取物作为潜在的皮肤增白成分提供依据。
创新点:发现旋覆花植物乳杆菌发酵的提取物可以作为有效的美白材料。
方法:比较研究了植物乳杆菌发酵的旋覆花花瓣提取物对酪氨酸酶活性的抑制作用,并测定了提取物中总黄酮和多酚类化合物的含量。通过体外酪氨酸酶和左旋多巴(L-DOPA)染色来测定其对皮肤的美白活性。
结论:旋覆花花瓣经过72小时的发酵后,其提取物中总黄酮含量提高13.4%。采用500 µg/ml发酵和未发酵的提取物处理B16F10细胞,细胞活性分别为100.26%和92.15%。此外,发酵72小时后,该提取物对酪氨酸酶活性的抑制率由29.33%上升到41.74%。未处理的对照组B16F10细胞酪氨酸酶活性增加到145.69%。结果表明,旋覆花发酵提取物对酪氨酸酶活性呈剂量依赖性抑制是促黑激素(α-MSH)的刺激引起。旋覆花植物乳杆菌发酵的提取物可以作为有效的美白材料。

关键词:旋覆花;类黄酮;植物乳杆菌;抗氧化剂;酪氨酸酶抑制剂

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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