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CLC number: R36

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 2017-08-16

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Yi-ping Tian

http://orcid.org/0000-0001-8218-4402

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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.9 P.747-756

http://doi.org/10.1631/jzus.B1600314


Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer


Author(s):  Yi-ping Tian, Ai-fen Lin, Mei-fu Gan, Hao Wang, Dan Yu, Chong Lai, Dan-dan Zhang, Yi-min Zhu, Mao-de Lai

Affiliation(s):  Department of Pathology, School of Medicine, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   zhuym@zju.edu.cn, lmd@zju.edu.cn

Key Words:  5-Hydroxymethylcytosine (5hmC), 5-Methylcytosine (5mC), Prognosis, Colorectal cancer


Yi-ping Tian, Ai-fen Lin, Mei-fu Gan, Hao Wang, Dan Yu, Chong Lai, Dan-dan Zhang, Yi-min Zhu, Mao-de Lai. Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer[J]. Journal of Zhejiang University Science B, 2017, 18(9): 747-756.

@article{title="Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer",
author="Yi-ping Tian, Ai-fen Lin, Mei-fu Gan, Hao Wang, Dan Yu, Chong Lai, Dan-dan Zhang, Yi-min Zhu, Mao-de Lai",
journal="Journal of Zhejiang University Science B",
volume="18",
number="9",
pages="747-756",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600314"
}

%0 Journal Article
%T Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer
%A Yi-ping Tian
%A Ai-fen Lin
%A Mei-fu Gan
%A Hao Wang
%A Dan Yu
%A Chong Lai
%A Dan-dan Zhang
%A Yi-min Zhu
%A Mao-de Lai
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 9
%P 747-756
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600314

TY - JOUR
T1 - Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer
A1 - Yi-ping Tian
A1 - Ai-fen Lin
A1 - Mei-fu Gan
A1 - Hao Wang
A1 - Dan Yu
A1 - Chong Lai
A1 - Dan-dan Zhang
A1 - Yi-min Zhu
A1 - Mao-de Lai
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 9
SP - 747
EP - 756
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600314


Abstract: 
Aberrant DNA methylation has raised widespread attention in tumorigenesis. In this study, we aimed to investigate the changes of global DNA methylation and hydroxymethylation from normal to tumor tissues in colorectal cancer (CRC) and their association with the prognosis. The levels of genomic 5-Hydroxymethylcytosine (5hmC) and 5-Methylcytosine (5mC) in cancerous tissues were significantly lower than those in corresponding adjacent normal tissues. The genomic levels of 5mC were significantly positively correlated with 5hmC in normal and cancerous tissues (all P<0.05). The ratio of 5mC in cancerous tissues to matched normal tissues (C/N-5mC) was also significantly positively correlated with the ratio of 5hmC in cancerous tissues to matched normal tissues (C/N-5hmC) (P=0.01). The 5mC levels and C/N-5mC ratios decreased with age (all P<0.05). Higher 5mC and 5hmC levels were found in rectal than in colon tissues (all P<0.05). High levels of 5mC in cancerous tissues and high C/N-5hmC ratios were each associated with lymph node metastasis (all P<0.05). Survival analysis indicated that the C/N-5mC ratio (P=0.04) is an independent protective factor for overall survival. The data showed that patients with a combination of high C/N-5hmC and low C/N-5mC ratios tended to have a worse prognosis (P<0.01). Our findings showed that the C/N-5mC ratio may be an independent prognostic factor for CRC outcome. Patients with both a high C/N-5hmC ratio and a low C/N-5mC ratio exhibited the worst survival, suggesting that 5mC and 5hmC can be used as critical markers in tumorigenesis and prognosis estimation.

正常组织与肿瘤组织中总体5hmC和5mC水平的变化程度与结直肠癌肿瘤发生及预后有关

目的:研究DNA总体甲基化、羟甲基化水平与结直肠癌发生、发展及预后的关系。
创新点:采用肿瘤与正常组织总体5-甲基胞嘧啶(5mC)、5-羟甲基胞嘧啶(5hmC)的比值并探讨其与结直肠癌临床病理变化和预后的关系。
方法:采用酶联免疫吸附试验(ELISA)方法检测肿瘤与相应正常组织基因组中总体5hmC和5mC水平。
结论:C/N-5mC比值的水平对结直肠癌的预后是一个独立的保护性因子(P=0.04)。当病人同时具有高水平的C/N-5hmC和低水平的C/N-5mC,其预后更差(P<0.01)。因此,可以使用5mC和5hmC对结直肠癌的发生和预后进行评估。

关键词:5-羟甲基胞嘧啶;5-甲基胞嘧啶;预后;结直肠癌

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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